Improved blood RNA extraction microtechnique for RT-PCR.
AuthorsZhang Y, Yunis JJ
JournalBiotechniques
PubMed ID7619479
n/a
Cloning, expression, and characterization of polyphosphate glucokinase from Mycobacterium tuberculosis.
AuthorsHsieh PC, Shenoy BC, Samols D, Phillips NF
JournalJ Biol Chem
PubMed ID8617763
Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor. The M. tuberculosis H37Rv gene encoding this enzyme has been cloned, sequenced, and expressed in Escherichia coli. The gene contains an open reading frame for 265 amino acids with a calculated mass ... More
Characterization of functional domains within the multifunctional transcription factor, YY1.
AuthorsBushmeyer S, Park K, Atchison ML
JournalJ Biol Chem
PubMed ID8530432
YY1 is a multifunctional transcription factor capable of either activation or repression of transcription. Using a series of mutant proteins, we have characterized domains responsible for activation or repression. We found that the YY1 transcriptional activation domain lies near the amino terminus and requires amino acids 16-29 and 80-100 for ... More
Water-soluble, recombinant CuA-domain of the cytochrome ba3 subunit II from Thermus thermophilus.
Recently, the genes of cytochrome ba3 from thermus thermophilus [Keightley, J.A., et al. (1995) J. Biol. Chem. 270, 20345-20358], a homolog of the heme-copper oxidase family, have been cloned. We report here expression of a truncated gene, encoding the copper A (CuA) domain of cytochrome ba3, that is regulated by ... More
Selective induction of neuropilin-1 by vascular endothelial growth factor (VEGF): a mechanism contributing to VEGF-induced angiogenesis.
Neuropilin (NRP) 1, previously identified as a neuronal receptor that mediates repulsive growth cone guidance, has been shown recently to function also in endothelial cells as an isoform-specific receptor for vascular endothelial growth factor (VEGF)(165) and as a coreceptor in vitro of VEGF receptor 2. However, its potential role in ... More