Common intra-articular T cell expansions in patients with reactive arthritis: identical beta-chain junctional sequences and cytotoxicity toward HLA-B27.
JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID10201900
Alternatively spliced transcripts of the human complement C2 gene.
AuthorsCheng J, Volanakis JE
JournalJ Immunol
PubMed ID8120386
To investigate the possible presence of alternatively spliced C2 gene transcripts, we amplified mRNA from HepG2 cells by reverse transcription-PCR using primers derived from the 5' and 3' untranslated regions of the C2 mRNA. Cloning of the resulting products revealed the presence of four novel C2 mRNA size variants. Nucleotide ... More
Alternative splicing of human immunodeficiency virus type 1 mRNA modulates viral protein expression, replication, and infectivity.
AuthorsPurcell DF, Martin MA
JournalJ Virol
PubMed ID8411338
Multiple RNA splicing sites exist within human immunodeficiency virus type 1 (HIV-1) genomic RNA, and these sites enable the synthesis of many mRNAs for each of several viral proteins. We evaluated the biological significance of the alternatively spliced mRNA species during productive HIV-1 infections of peripheral blood lymphocytes and human ... More
A 127 kDa component of a UV-damaged DNA-binding complex, which is defective in some xeroderma pigmentosum group E patients, is homologous to a slime mold protein.
AuthorsTakao M, Abramic M, Moos M Jr, Otrin VR, Wootton JC, McLenigan M, Levine AS, Protic M
JournalNucleic Acids Res
PubMed ID8371985
'A cDNA which encodes a approximately 127 kDa UV-damaged DNA-binding (UV- DDB) protein with high affinity for (6-4)pyrimidine dimers [Abramic'', M., Levine, A.S. & Protic'', M., J. Biol. Chem. 266: 22493-22500, 1991] has been isolated from a monkey cell cDNA library. The presence of this protein in complexes bound to ... More
A null mutation in the perforin gene impairs cytolytic T lymphocyte- and natural killer cell-mediated cytotoxicity.
AuthorsLowin B, Beermann F, Schmidt A, Tschopp J
JournalProc Natl Acad Sci U S A
PubMed ID7972104
'Lymphocyte-mediated cytotoxicity has been proposed to consist of the polarized secretion of granule-stored perforin leading to target-cell lysis. Nevertheless, perforin-independent pathways were postulated to explain the cytolytic activity of apparently perforin-free lymphocytes and the DNA degradation found in dying target cells. To evaluate the role of perforin, we used gene ... More
X inactivation in mammalian testis is correlated with inactive X- specific transcription.
AuthorsRichler C, Soreq H, Wahrman J
JournalNat Genet
PubMed ID1345167
'X chromosome inactivation occurs twice during the mammalian life cycle. In females one of the two X chromosomes of somatic nuclei is inactive, while in males the solitary X chromosome is inactivated during germ cell development. Despite the different properties of the inactivated chromosomes of females and males, the molecular ... More
The beta subunit of the mitochondrial processing peptidase from rat liver: cloning and sequencing of a cDNA and comparison with a proposed family of metallopeptidases.
AuthorsPaces V, Rosenberg LE, Fenton WA, Kalousek F
JournalProc Natl Acad Sci U S A
PubMed ID8506385
'Most nuclearly encoded mitochondrial proteins are synthesized with amino-terminal leader peptides that are removed by the mitochondrial processing peptidase (MPP) after translocation. Earlier we reported cloning and sequencing of a cDNA for the larger subunit (MPP alpha subunit) of this enzyme from rat liver mitochondria. We have now completed the ... More
Macrophage inflammatory protein-1beta induces migration and activation of human thymocytes.
AuthorsDairaghi DJ, Franz-Bacon K, Callas E, Cupp J, Schall TJ, Tamraz SA, Boehme SA, Taylor N, Bacon KB
JournalBlood
PubMed ID9531601
'The CC chemokine macrophage inflammatory protein 1beta (MIP-1beta), has been shown to be a chemoattractant preferentially activating CD4(+) CD45RA+ T lymphocytes. Further analysis of chemokine action on lymphocytic cells has shown the potent migration-promoting capacity of MIP-1beta on human thymocytes. The responding cells were the CD4(+) and CD8(+) single-positive (SP), ... More
Isolation of a candidate gene for Menkes disease and evidence that it encodes a copper-transporting ATPase [published erratum appears in Nat Genet 1993 Mar;3(3):273] [see comments]
'Menkes disease is an X-linked disorder of copper transport characterized by progressive neurological degeneration and death in early childhood. We have isolated a candidate gene (Mc1) for Menkes disease and find qualitative or quantitative abnormalities in the mRNA in sixteen of twenty-one Menkes patients. Four patients lacking Mc1RNA showed rearrangements ... More
DNA methylation in transcriptional repression of two differentially expressed X-linked genes, GPC3 and SYBL1.
AuthorsHuber R, Hansen RS, Strazzullo M, Pengue G, Mazzarella R, D'Urso M, Schlessinger D, Pilia G, Gartler SM, D'Esposito M
JournalProc Natl Acad Sci U S A
PubMed ID9892682
'Methylation of CpG islands is an established transcriptional repressive mechanism and is a feature of silencing in X chromosome inactivation. Housekeeping genes that are subject to X inactivation exhibit differential methylation of their CpG islands such that the inactive alleles are hypermethylated. In this report, we examine two contrasting X-linked ... More
The mottled gene is the mouse homologue of the Menkes disease gene [see comments]
The mottled mouse has been proposed as an animal model for Menkes disease, an X-linked disorder of copper transport. The recent isolation of a copper-transporting ATPase gene responsible for Menkes disease has allowed us to test this hypothesis. Here we report the isolation and sequence of the mouse homologue of ... More
Targeted gene disruption shows that knobs enable malaria-infected red cells to cytoadhere under physiological shear stress.
AuthorsCrabb BS, Cooke BM, Reeder JC, Waller RF, Caruana SR, Davern KM, Wickham ME, Brown GV, Coppel RL, Cowman AF
JournalCell
PubMed ID9108483
Knobs at the surface of erythrocytes infected with Plasmodium falciparum have been proposed to be important in adherence of these cells to the vascular endothelium. This structure contains the knob- associated histidine-rich protein (KAHRP) and the adhesion receptor P. falciparum erythrocyte membrane protein 1. We have disrupted the gene encoding ... More
Molecular analysis of herpes simplex virus type 1 during epinephrine- induced reactivation of latently infected rabbits in vivo.
AuthorsBloom DC, Devi-Rao GB, Hill JM, Stevens JG, Wagner EK
JournalJ Virol
PubMed ID8107194
Infectious virus assays and PCR amplification of DNA and RNA were used to investigate herpes simplex virus (HSV) DNA replication and gene expression in the rabbit corneal model for virus reactivation in vivo. We used carefully defined latency-associated transcript-negative (LAT-) and LAT+ promoter mutants of the 17syn+ strain of HSV ... More
The serine protease cofactor factor V is synthesized by lymphocytes 1
Ag-specific cellular immune responses result in CD4+ T cell activation, which can induce the expression of tissue factor in cells of monocyte/macrophage lineage. This results in initiation of the coagulation protease cascade, with ultimate generation of thrombin. The latter is a potent and pleiotropic mediator of cellular responses and deposition ... More
Myeloid expression of cytochrome P450 4F3 is determined by a lineage-specific alternative promoter.
The cytochrome P450 4F3 (CYP4F3) gene encodes two functionally distinct enzymes that differ only by the selection of exon 4 (CYP4F3A) or exon 3 (CYP4F3B). CYP4F3A inactivates leukotriene B4, a reaction that has significance for controlling inflammation. CYP4F3B converts arachidonic acid to 20-hydroxyeicosatetraenoic acid, a potent activator of protein kinase ... More
Primary structure, neural-specific expression, and dendritic location of human BC200 RNA.
AuthorsTiedge H, Chen W, Brosius J
JournalJ Neurosci
PubMed ID7684772
Primate BC200 RNA is a 200-nucleotide-long, nontranslatable RNA that is prevalently expressed in the nervous system. We have determined the primary structure of human BC200 RNA, using cDNA cloning and PCR techniques. BC200 RNA can be subdivided into three structural domains. The 5' region is homologous to Alu repetitive elements ... More
Functional characterization of B lymphocytes generated in vitro from embryonic stem cells
To study molecular events involved in B lymphocyte development and V(D)J rearrangement, we have established an efficient system for the differentiation of embryonic stem (ES) cells into mature Ig-secreting B lymphocytes. Here, we show that B lineage cells generated in vitro from ES cells are functionally analogous to normal fetal ... More