Use of enhanced green fluorescent protein to optimize and quantitate infection of target cells with recombinant retroviruses.
AuthorsCashion LM, Bare LA, Harvey S, Trinh Q, Zhu Y, Devlin JJ
JournalBiotechniques
PubMed ID10337486
'Recombinant retroviral vectors are useful tools for gene transfer in both gene therapy and research applications. An enhanced form of green fluorescent protein has been incorporated into recombinant retroviruses as a marker to follow infected cells. In this paper, we extended the use of the fluorescent reporter to quantify protein ... More
Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.
AuthorsGhanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,
JournalJ Immunol
PubMed ID15100314
Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More
Phosphorylation of serine 256 is required for cAMP-dependent regulatory exocytosis of the aquaporin-2 water channel.
AuthorsFushimi K, Sasaki S, Marumo F
JournalJ Biol Chem
PubMed ID9169447
The aquaporin-2 (AQP2) vasopressin water channel is translocated to the apical membrane upon vasopressin stimulation. Phosphorylation of serine 256 of AQP2 by cAMP-dependent protein kinase has been shown, but its relation to vasopressin-regulated translocation has not been elucidated. To address this question, wild type (WT) AQP2 and a mutant with ... More