EpiJET 5-hmC and 5-mC Analysis Kit - FAQs

查看更多产品信息 EpiJET 5-hmC and 5-mC Analysis Kit - FAQs (K1501)

4 个常见问题解答

What is the difference between EpiJET 5-hmC Analysis Kit (Cat. No. K1481) and EpiJET 5-hmC and 5-mC Analysis Kit (Cat. No. K1501)?

The new EpiJET 5-hmC and 5-mC Analysis Kit is more universal than the previous EpiJET 5-hmC Analysis Kit because it provides a total assessment of both cytosine modifications - 5-hydroxymethylcytosine (5-hmC) and methylcytosine (5-mC) - within specific DNA loci (for comparison - the current kit allows only 5-hmC analysis). Additionally, all necessary DNA controls do not originate from plasmids widely used in molecular biology laboratories are included into the new kit.

How much DNA can I use for glucosylation and restriction digestion reactions with EpiJET 5-hmC and 5-mC Analysis Kit?

For each glucosylation reaction, a broad range of DNA input can be used from 50 ng to 1 µg.

Do I need an extra step of genomic DNA purification prior to using the sample in EpiJET 5-hmC and 5-mC Analysis Kit?

DNA purity is an important factor for successful digestion by Epi MspI/HpaII. We recommend use of spin column-based kits such as the Thermo Scientific GeneJET Genomic DNA Purification Kit (Cat. No. K0721/2) for genomic DNA purification.

Can I buy T4 B-glucosyltransferase separately from EpiJET 5-hmC and 5-mC Analysis Kit?

Yes, Thermo Scientific T4 B-glucosyltransferase is available as a stand-alone enzyme (Cat. No. EO0831, 500 U).