RiboMinus™ 转录组分离试剂盒,酵母菌
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Invitrogen™

RiboMinus™ 转录组分离试剂盒,酵母菌

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal了解更多信息
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货号 K155003
价格(CNY)
4,598.00
Each
添加至购物车
价格(CNY)
4,598.00
Each
添加至购物车
RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5μ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.
规格
最终产品类型转录组 RNA
适用于(应用)微阵列分析、实时荧光定量 PCR (qPCR)、逆转录酶 PCR (RT-PCR)、Northern 印迹、cDNA 文库构建
形式磁珠
高通量能力不兼容高通量应用(手动)
反应次数12 reactions
产品线RiboMinus™
纯化时间1.25 个小时
数量12 reactions
原始材料量多达 10 µg 总 RNA
系统类型RiboMinus
产量1 µg
分离技术磁珠
样品类型总 RNA(酵母菌)
目标转录组 RNA
Unit SizeEach

常见问题解答 (FAQ)

使用RiboMinus试剂盒提取的RNA,其最佳分析方法是什么?

纯化后的RNA可以方便的用260 nm 紫外吸光值或Quant-iT RNA Assay试剂盒进行定量。要验证rRNA的去除效果,可以对样本进行琼脂糖凝胶电泳检测或使用Agilent 2100 Bioanalyzer进行分析。与对照相比,样本的琼脂糖凝胶电泳应无18S和28S rRNA条带。通过琼脂糖凝胶电泳也可以判断样品是否有DNA污染及RNA降解。rRNA的去除效率,RNA的降解,以及RNA的浓度也都可以通过Bioanalyzer进行分析。

你们的可用于RNA-seq的RiboMinus 真核试剂盒都在哪些物种上做过检测?

对于物种特异性来说,以下物种对于LSU/SSU探针没有任何错配(LSU (28S) / SSU (18S)探针完美配对)。这意味着探针可从下列物种中去除18S和28S序列。

陆生物种:Human, mouse, rat, frog, rabbit, cow, pig, chicken, Drosophila,* Caenorhabditis elegans, midge, mosquito, yeast (Saccharomyces cerevisiae, Schizosaccharomyces pombe)
海洋物种:Sea squirt, coelacanth, lancelet, eel, salmon, sturgeon, ratfish, lamprey, brown plankton, zooplankton
植物:Arabidopsis thaliana, Brassica napus, rice, tomato, Humulus lupulus, Zea mays, wheat, soybean, pine, aspen

* 该试剂盒中的28S和18S探针分别和果蝇的 18S和28S rRNA序列完全匹配。但是,有报道表明果蝇(昆虫)的 28S rRNA会分裂为2个较小片段并与18S共迁移。目前准确的分裂位点还未被找到。但是,基于用户的反馈,该试剂盒并不能完全去除分裂的28S rRNA。我们也没有用该试剂盒测试过果蝇样本。

5S和5.8S序列更具多样性,因此效果好的探针所使用的物种范围可能会较小:
5S: 0至1个错配:人,小鼠,大鼠,青蛙,鸡,果蝇,线虫, 拟南芥,玉米
5.8S: 0错配:人, 小鼠,大鼠

What is the best way to analyze RNA that I've isolated using a RiboMinus kit?

The purified RNA is easily quantitated using UV absorbance at 260 nm or a Quant-iT RNA Assay Kit. To verify rRNA depletion, electrophorese your sample on an agarose gel or use an Agilent 2100 Bioanalyzer. Agarose gel electrophoresis should show depletion of 18S and 28S rRNA bands compared to a control sample. Absence of contaminating DNA and RNA degradation may also be confirmed by agarose gel electrophoresis. The efficiency of rRNA depletion in the purified RNA, RNA degradation, and RNA concentration can also be analyzed using a bioanalyzer.

What species have you tested with your RiboMinus Eukaryote Kit for RNA-Seq?

With regard to species specificity, the following organisms have 0 mismatches for the LSU/SSU probes (LSU (28S) / SSU (18S) Probe perfect matches). This means the probes will remove the 18S and 28S sequences from the following species.

- Species from the Land: Human, mouse, rat, frog, rabbit, cow, pig, chicken, Drosophila,* Caenorhabditis elegans, midge, mosquito, yeast (Saccharomyces cerevisiae, Schizosaccharomyces pombe)
- Species from the Sea: Sea squirt, coelacanth, lancelet, eel, salmon, sturgeon, ratfish, lamprey, brown plankton, zooplankton
- Plants: Arabidopsis thaliana, Brassica napus, rice, tomato, Humulus lupulus, Zea mays, wheat, soybean, pine, aspen

* The 28S and 18S probes in this kit respectively align 100% to the Drosophila 18S and 28S rRNA sequence. However, there are reports suggesting Drosophila (insect) 28S rRNA split into 2 smaller fragments co-migrating with 18S. The exact split site is not identified yet. But based on customer feedback, this kit does not remove the split 28S rRNA completely. We have not tested Drosophila samples with this kit.

The 5S and 5.8S sequences are more divergent, and thus the best probes possible have reduced species breadth:
- 5S: zero to 1 mismatch: human, mouse, rat, frog, chicken, Drosophila, C. elegans, A. thaliana, Z. mays
- 5.8S: zero mismatches: human, mouse, rat