RiboMinus™ Concentration Module - FAQs

查看更多产品信息 RiboMinus™ Concentration Module - FAQs (K155005)

4 个常见问题解答

使用RiboMinus试剂盒分离纯化RNA，得到的RNA产量很低。这是什么原因引起的？

提取的RNA量本身就比较少，或者在乙醇沉淀过程中将RNA沉淀丢失，都可能导致RNA产量低。另外，不同组织的RNA含量不同，RNA的产量取决于样本的种类。

使用RiboMinus试剂盒提取的RNA，其最佳分析方法是什么？

纯化后的RNA可以方便的用260 nm 紫外吸光值或Quant-iT RNA Assay试剂盒进行定量。要验证rRNA的去除效果，可以对样本进行琼脂糖凝胶电泳检测或使用Agilent 2100 Bioanalyzer进行分析。与对照相比，样本的琼脂糖凝胶电泳应无18S和28S rRNA条带。通过琼脂糖凝胶电泳也可以判断样品是否有DNA污染及RNA降解。rRNA的去除效率，RNA的降解，以及RNA的浓度也都可以通过Bioanalyzer进行分析。

I'm getting low RNA yield after using a RiboMinus kit for RNA extraction. What could be the cause of this?

Low RNA content or a loss of the pellet during ethanol precipitation could lead to a low RNA yield. Additionally, various tissues have different RNA content, and the yield is dependent on the sample.

What is the best way to analyze RNA that I've isolated using a RiboMinus kit?

The purified RNA is easily quantitated using UV absorbance at 260 nm or a Quant-iT RNA Assay Kit. To verify rRNA depletion, electrophorese your sample on an agarose gel or use an Agilent 2100 Bioanalyzer. Agarose gel electrophoresis should show depletion of 18S and 28S rRNA bands compared to a control sample. Absence of contaminating DNA and RNA degradation may also be confirmed by agarose gel electrophoresis. The efficiency of rRNA depletion in the purified RNA, RNA degradation, and RNA concentration can also be analyzed using a bioanalyzer.