RNA editing generates a diverse array of transcripts encoding squid Kv2 K+ channels with altered functional properties.
AuthorsPatton DE, Silva T, Bezanilla F
JournalNeuron
PubMed ID9331360
We have cloned a Kv2 potassium channel from squid optic lobe termed sqKv2. Multiple overlapping sqKv2 cDNA clones differed from one another at specific positions by purine transitions. To test whether the purine transitions were generated by RNA editing, we compared a 360 nucleotide genomic sequence with corresponding cDNA sequences ... More
Structure and expression of the membrane proteoglycan betaglycan, a component of the TGF-beta receptor system.
AuthorsLopez-Casillas F, Cheifetz S, Doody J, Andres JL, Lane WS, Massague J
JournalCell
PubMed ID1657406
We describe the primary structure of rat betaglycan, a polymorphic membrane-anchored proteoglycan with high affinity for transforming growth factor-beta (TGF-beta). As deduced from its cDNA sequence, the 853 amino acid core protein of betaglycan has an extracellular domain with clustered sites for potential attachment of glycosaminoglycan chains. These chains are ... More
Stereotyped and specific gene expression programs in human innate immune responses to bacteria.
AuthorsBoldrick Jennifer C; Alizadeh Ash A; Diehn Maximilian; Dudoit Sandrine; Liu Chih Long; Belcher Christopher E; Botstein David; Staudt Louis M; Brown Patrick O; Relman David A;
JournalProc Natl Acad Sci U S A
PubMed ID11805339
The innate immune response is crucial for defense against microbial pathogens. To investigate the molecular choreography of this response, we carried out a systematic examination of the gene expression program in human peripheral blood mononuclear cells responding to bacteria and bacterial products. We found a remarkably stereotyped program of gene ... More
Transformation of follicular lymphoma to diffuse large-cell lymphoma: alternative patterns with increased or decreased expression of c-myc and its regulated genes.
Authors Lossos Izidore S; Alizadeh Ash A; Diehn Maximilian; Warnke Roger; Thorstenson Yvonne; Oefner Peter J; Brown Patrick O; Botstein David; Levy Ronald;
JournalProc Natl Acad Sci U S A
PubMed ID12077300
'The natural history of follicular lymphoma (FL) is frequently characterized by transformation to a more aggressive diffuse large B cell lymphoma (DLBCL). We compared the gene-expression profiles between transformed DLBCL and their antecedent FL. No genes were observed to increase or decrease their expression in all of the cases of ... More
HERF1, a novel hematopoiesis-specific RING finger protein, is required for terminal differentiation of erythroid cells.
AuthorsHarada H, Harada Y, O'Brien DP, Rice DS, Naeve CW, Downing JR
JournalMol Cell Biol
PubMed ID10207104
'The AML1/core binding factor beta (CBFbeta) transcription factor is essential for definitive hematopoiesis; however, the downstream pathways through which it functions remain incompletely defined. Using a differential cloning approach to define components of this pathway, we have identified a novel gene designated HERF1 (for hematopoietic RING finger 1), whose expression ... More
Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK inhibitory receptor.
AuthorsIkeda H, Lethe B, Lehmann F, van Baren N, Baurain JF, de Smet C, Chambost H, Vitale M, Moretta A, Boon T, Coulie PG
JournalImmunity
PubMed ID9047241
'Melanoma lines MEL.A and MEL.B were derived from metastases removed from patient LB33 in 1988 and 1993, respectively. The MEL.A cells express several antigens recognized by autologous cytolytic T lymphocytes (CTL) on HLA class I molecules. The MEL.B cells have lost expression of all class I molecules except for HLA-A24. ... More
The identification and functional characterization of a novel mast cell isoform of the microphthalmia-associated transcription factor.
AuthorsTakemoto CM, Yoon YJ, Fisher DE.
JournalJ Biol Chem
PubMed ID12039954
'The microphthalmia-associated transcription factor (Mitf) is critical for mast cell development based on the severe mast cell deficiency seen in Mitf mutant mice. Mitf also is important for the development of melanocytes, osteoclasts, and retinal pigment epithelium. The lineage-restricted phenotypes of Mitf mutations correlate with tissue-restricted expression of Mitf, a ... More
P2X4 Receptor Is a Glycosylated Cardiac Receptor Mediating a Positive Inotropic Response to ATP.
Authors Hu Bing; Senkler Carol; Yang Alexander; Soto Florentina; Liang Bruce T;
JournalJ Biol Chem
PubMed ID11864978
'Although P2X receptors are suggested to play a role in synaptic neurotransmission, the specific physiological role of each P2X receptor subtype remains largely unknown. We used cultured chick embryo ventricular myocytes as a model to study a potential physiological role of the P2X(4) receptor in mediating the positive inotropic effect ... More
Nitric oxide activates diverse signaling pathways to regulate gene expression.
AuthorsHemish J, Nakaya N, Mittal V, Enikolopov G,
JournalJ Biol Chem
PubMed ID12907672
'Nitric oxide signaling is crucial for effecting long lasting changes in cells, including gene expression, cell cycle arrest, apoptosis, and differentiation. We have determined the temporal order of gene activation induced by NO in mammalian cells and have examined the signaling pathways that mediate the action of NO. Using microarrays ... More
Identification of Nogo-66 receptor (NgR) and homologous genes in fish.
AuthorsKlinger M, Taylor JS, Oertle T, Schwab ME, Stuermer CA, Diekmann H,
JournalMol Biol Evol
PubMed ID12949137
'The Nogo-66 receptor NgR has been implicated in the mediation of inhibitory effects of central nervous system (CNS) myelin on axon growth in the adult mammalian CNS. NgR binds to several myelin-associated ligands (Nogo-66, myelin associated glycoprotein, and oligodendrocyte-myelin glycoprotein), which, among other inhibitory proteins, impair axonal regeneration in the ... More
Expression cloning and function of the rat NK activating and inhibitory receptors NKR-P1A and -P1B.
AuthorsLi J, Rabinovich BA, Hurren R, Shannon J, Miller RG,
JournalInt Immunol
PubMed ID12618485
'We have characterized the rat NK receptors NKR-P1A and -P1B. A cDNA library was constructed from the rat NK cell line, RNK-16. Using the pMX retroviral cloning system, the library was expressed in the human NK cell line, YTSeco, and cells staining with the anti-rat mAb 10/78 identified, FACS sorted ... More
Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line.
Authors Kekuda R; Prasad P D; Fei Y J; Torres-Zamorano V; Sinha S; Yang-Feng T L; Leibach F H; Ganapathy V;
JournalJ Biol Chem
PubMed ID8702519
'We have isolated a cDNA from a human placental choriocarcinoma cell cDNA library which, when expressed in HeLa cells, induces a Na+-dependent amino acid transport system with preference for zwitterionic amino acids. Anionic amino acids, cationic amino acids, imino acids, and N-methylated amino acids are excluded by this system. These ... More
UNR translation can be driven by an IRES element that is negatively regulated by polypyrimidine tract binding protein.
AuthorsCornelis S, Tinton SA, Schepens B, Bruynooghe Y, Beyaert R,
JournalNucleic Acids Res
PubMed ID15928332
'Upstream of N-ras (Unr) has been described as an internal initiation trans-acting factor (ITAF) in the cap-independent translation of some particular viral and cellular mRNAs. Two factors led us to hypothesize that the UNR 5''-untranslated region (5''-UTR) may contain an internal ribosome entry site (IRES). The first was the requirement ... More
A common mutational pattern in Cockayne syndrome patients from xeroderma pigmentosum group G: implications for a second XPG function.
AuthorsNouspikel T, Lalle P, Leadon SA, Cooper PK, Clarkson SG
JournalProc Natl Acad Sci U S A
PubMed ID9096355
'Xeroderma pigmentosum (XP) patients have defects in nucleotide excision repair (NER), the versatile repair pathway that removes UV-induced damage and other bulky DNA adducts. Patients with Cockayne syndrome (CS), another rare sun-sensitive disorder, are specifically defective in the preferential removal of damage from the transcribed strand of active genes, a ... More
Ongoing immunoglobulin somatic mutation in germinal center B cell-like but not in activated B cell-like diffuse large cell lymphomas.
AuthorsLossos IS, Alizadeh AA, Eisen MB, Chan WC, Brown PO, Botstein D, Staudt LM, Levy R
JournalProc Natl Acad Sci U S A
PubMed ID10954754
'B cell diffuse large cell lymphoma (B-DLCL) is a heterogeneous group of tumors, based on significant variations in morphology, clinical presentation, and response to treatment. Gene expression profiling has revealed two distinct tumor subtypes of B-DLCL: germinal center B cell-like DLCL and activated B cell-like DLCL. In a separate study, ... More
Upstream organization of and multiple transcripts from the human folylpoly-gamma-glutamate synthetase gene.
AuthorsFreemantle SJ, Taylor SM, Krystal G, Moran RG
JournalJ Biol Chem
PubMed ID7721888
'Folylpoly-gamma-glutamate synthetase (FPGS) is essential for the survival of proliferating mammalian cells and central to the action of all classical folate antimetabolites. We report the isolation of cDNAs corresponding to the 5'' ends of FPGS mRNA from both human and hamster cells which include a start codon upstream of and ... More
A 127 kDa component of a UV-damaged DNA-binding complex, which is defective in some xeroderma pigmentosum group E patients, is homologous to a slime mold protein.
AuthorsTakao M, Abramic M, Moos M Jr, Otrin VR, Wootton JC, McLenigan M, Levine AS, Protic M
JournalNucleic Acids Res
PubMed ID8371985
'A cDNA which encodes a approximately 127 kDa UV-damaged DNA-binding (UV- DDB) protein with high affinity for (6-4)pyrimidine dimers [Abramic'', M., Levine, A.S. & Protic'', M., J. Biol. Chem. 266: 22493-22500, 1991] has been isolated from a monkey cell cDNA library. The presence of this protein in complexes bound to ... More
Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation.
AuthorsNinomiya-Tsuji J, Nomoto S, Yasuda H, Reed SI, Matsumoto K
JournalProc Natl Acad Sci U S A
PubMed ID1717994
'We have cloned two different human cDNAs that can complement cdc28 mutations of budding yeast Saccharomyces cerevisiae. One corresponds to a gene encoding human p34CDC2 kinase, and the other to a gene (CDK2; cell division kinase) that has not been characterized previously. The CDK2 protein is highly homologous to p34CDC2 ... More
Cloning and characterization of a family of proteins associated with Mpl.
'Thrombopoietin (TPO) controls the formation of megakaryocytes and platelets from hematopoietic stem cells via activation of the c-Mpl receptor and multiple downstream signal transduction pathways. We used two-hybrid screening to identify new proteins that interacted with the cytoplasmic domain of Mpl, and we found a new family of proteins designated ... More
A receptor for phosphatidylserine-specific clearance of apoptotic cells
AuthorsFadok VA, Bratton DL, Rose DM, Pearson A, Ezekewitz RA, Henson PM
JournalScience
PubMed ID10811223
'The culmination of apoptosis in vivo is phagocytosis of cellular corpses. During apoptosis, the asymmetry of plasma membrane phospholipids is lost, which exposes phosphatidylserine externally. During apoptosis, the asymmetry of plasma membrane phospholipids is lost, which exposes phosphatidylserine externally. The phagocytosis of apoptotic cells can be inhibited stereospecifically by ... More
Transcriptional induction of the melanocyte-stimulating hormone receptor in brain metastases of murine K-1735 melanoma.
'Metastatic K-1735 murine melanoma cells are amelanotic in culture or in the subcutis of syngeneic mice. When injected into the internal carotid artery, these cells produce melanotic brain metastases. The production of melanin in tumor cells growing in the brain was directly correlated with induction of melanocyte-stimulating hormone receptor (MSH-R) ... More
Cloning of ELL, a gene that fuses to MLL in a t(11;19)(q23;p13.1) in acute myeloid leukemia.
AuthorsThirman MJ, Levitan DA, Kobayashi H, Simon MC, Rowley JD
JournalProc Natl Acad Sci U S A
PubMed ID7991593
'To characterize the functions of MLL fusion transcripts, we cloned the gene that fuses to MLL in the translocation t(11;19)(q23;p13.1). This translocation is distinct from another type of 11;19 translocation with a 19p13.3 breakpoint that results in the fusion of MLL to the ENL gene. By PCR screening of a ... More
Regulation of sialomucin production in colon carcinoma cells.
'In this study, we investigated the biochemical basis for the induction of sialomucin production in HT-29 LMM human colon carcinoma cells. Previous studies showed that conditioned medium from organ cultures of normal human colonic connective tissues (NCCM) stimulated the biosynthesis of high molecular weight sialoglycoproteins (M(r) 740,000 and 450,000) by ... More
The influence of heregulins on human Schwann cell proliferation.
'The use of Schwann cell (SC) autotransplantation to influence neural repair in humans is dependent upon identifying mitogens that will effectively expand human Schwann cells (SCs) in culture. The recent purification and molecular cloning of glial growth factor (GGF), a potent mitogen for rat Schwann cells, has led to the ... More
NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus.
AuthorsMasuda ES, Naito Y, Tokumitsu H, Campbell D, Saito F, Hannum C, Arai K, Arai N
JournalMol Cell Biol
PubMed ID7739550
'The nuclear factor of activated T cells (NFAT) regulates cytokine gene expression in T cells through cis-acting elements located in the promoters of cytokine genes. Here, we report the cDNA cloning, chromosomal localization, and initial characterization of a transcription factor related to NFATp and NFATc. The novel molecule, designated NFATx, ... More
IFN-gamma-dependent IL-7 gene regulation in keratinocytes.
AuthorsAriizumi K, Meng Y, Bergstresser PR, Takashima A
JournalJ Immunol
PubMed ID7751645
'IL-7 plays a central role in regulating the growth and differentiation of T cells. We have reported previously that epidermal keratinocytes produce biologically relevant amounts of IL-7, thereby supporting the growth of epidermal gamma delta T cells. In this report, we report that IL-7 gene expression is regulated in keratinocytes ... More
Dystrophin is transcribed in brain from a distant upstream promoter.
AuthorsBoyce FM, Beggs AH, Feener C, Kunkel LM
JournalProc Natl Acad Sci U S A
PubMed ID1996328
'Dystrophin, the protein product of the Duchenne muscular dystrophy gene, is expressed in brain as well as muscle. The role of dystrophin in the brain is not clear, though one-third of Duchenne muscular dystrophy patients exhibit some degree of mental retardation. We have isolated the genomic region encoding the alternative ... More
Separation of sister chromatids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition.
AuthorsStratmann R, Lehner CF
JournalCell
PubMed ID8548823
'Mutations in the Drosophila genes pimples and three rows result in a defect of sister chromatid separation during mitosis. As a consequence, cytokinesis is also defective. However, cell cycle progression including the mitotic degradation of cyclins A and B is not blocked by the failure of sister chromatid separation, and ... More
Expression of human epileptic temporal lobe neurotransmitter receptors in Xenopus oocytes: An innovative approach to study epilepsy.
'Poly(A(+)) RNA was extracted from the temporal lobe (TL) of medically intractable epileptic patients which underwent surgical TL resection. Injection of this mRNA into Xenopus oocytes led to the expression of ionotropic receptors for gamma-aminobutyric acid (GABA), kainate (KAI) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA). ' ... More
Cloning and sequence analysis of a Bothrops jararaca cDNA encoding a precursor of seven bradykinin-potentiating peptides and a C-type natriuretic peptide.
AuthorsMurayama N, Hayashi MA, Ohi H, Ferreira LA, Hermann VV, Saito H, Fujita Y, Higuchi S, Fernandes BL, Yamane T, de Camargo AC
JournalProc Natl Acad Sci U S A
PubMed ID9037028
'A 1.8-kb cDNA clone was isolated from a Bothrops jararaca venom gland cDNA library that encodes a 256-aa precursor for bradykinin- potentiating peptides (angiotensin-converting enzyme inhibitors) and a C-type natriuretic peptide (CNP). The seven bradykinin-potentiating peptides are aligned tandemly after the hydrophobic signal peptide sequence, followed by a putative intervening ... More
An imprinted transcript, antisense to Nesp, adds complexity to the cluster of imprinted genes at the mouse Gnas locus.
'The Gnas locus in distal mouse chromosome (Chr) 2 is emerging as a complex genomic region. It contains three imprinted genes in the order Nesp-Gnasxl-Gnas. Gnas encodes a G protein alpha-subunit, and Nesp and Gnasxl encode proteins of unknown function expressed in neuroendocrine tissue. Together, these genes form a single ... More
Widespread expression of the human and rat Huntington's disease gene in brain and nonneural tissues.
AuthorsStrong TV, Tagle DA, Valdes JM, Elmer LW, Boehm K, Swaroop M, Kaatz KW, Collins FS, Albin RL
JournalNat Genet
PubMed ID8275091
'We have used RNA in situ hybridization to study the regional expression of the Huntington''s disease gene (HD) and its rat homologue in brain and selected nonneural tissues. The HD transcript was expressed throughout the brain in both rat and human, especially in the neurons of the dentate gyrus and ... More
Identification of a gene, MLL, that spans the breakpoint in 11q23 translocations associated with human leukemias [published erratum appears in Proc Natl Acad Sci U S A 1992 May 1;89(9):4220]
AuthorsZiemin-van der Poel S, McCabe NR, Gill HJ, Espinosa R III, Patel Y, Harden A, Rubinelli P, Smith SD, LeBeau MM, Rowley JD, et al
JournalProc Natl Acad Sci U S A
PubMed ID1720549
'Recurring chromosomal translocations involving chromosome 11, band q23, have been observed in acute lymphoid leukemias and especially in acute myeloid leukemias. We recently showed that breakpoints in four 11q23 translocations, t(4;11)(q21;q23), t(6;11)(q27;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13.3), were contained within a yeast artificial chromosome clone bearing the CD3D and CD3G gene loci. ... More
Simulation of human autosomal dominant retinitis pigmentosa in transgenic mice expressing a mutated murine opsin gene.
AuthorsNaash MI, Hollyfield JG, al-Ubaidi MR, Baehr W
JournalProc Natl Acad Sci U S A
PubMed ID8516292
'Autosomal dominant retinitis pigmentosa (ADRP), slowly progressing over decades, leads to severe visual impairment and in some cases to complete blindness. More than 40 mutations in the human opsin gene have been linked to some forms of this genetically heterogeneous disease. In photoreceptor cells of ADRP patients with mutations in ... More
CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene.
AuthorsRouvier E, Luciani MF, Mattei MG, Denizot F, Golstein P
JournalJ Immunol
PubMed ID8390535
'To detect novel molecules involved in immune functions, a subtracted cDNA library between closely related murine lymphoid cells was prepared using improved technology. Differential screening of this library yielded several clones with a very restricted tissue specificity, including one that we named CTLA-8. CTLA-8 transcripts could be detected only in ... More
Autoregulation of the human C/EBP alpha gene by stimulation of upstream stimulatory factor binding.
AuthorsTimchenko N, Wilson DR, Taylor LR, Abdelsayed S, Wilde M, Sawadogo M, Darlington GJ
JournalMol Cell Biol
PubMed ID7862113
'The human C/EBP alpha gene promoter shares significant sequence homology with that of the mouse but has a different mechanism of autoregulation. Activation of the murine promoter by direct binding of C/EBP alpha to a site within 200 bp of the transcriptional start was shown to elevate activity by approximately ... More
A Mammalian H+ Channel Generated Through Alternative Splicing of the NADPH Oxidase HomologNOH-1
AuthorsBanfi B, Maturana A, Jaconi S, Arnaudeau S, Laforge T, Sinha B, Ligeti E, Demaurex N, Krause KH
JournalScience
PubMed ID10615049
'Voltage-gated proton (H+) channels are found in many human and animal tissues and play an important role in cellular defense against acidic stress. However, a molecular identification of these unique ion conductances has so far not been achieved. A 191-amino acid protein is described that, upon heterologous expression, has properties ... More
Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transport-deficient cell line.
AuthorsCrawford CR, Patel DH, Naeve C, Belt JA
JournalJ Biol Chem
PubMed ID9478986
'Mammalian cells obtain nucleic acid precursors through the de novo synthesis of nucleotides and the salvage of exogenous nucleobases and nucleosides. The first step in the salvage pathway is transport across the plasma membrane. Several transport activities, including equilibrative and concentrative mechanisms, have been identified by their functional properties. We ... More
Identification of a new sea urchin ets protein, SpEts4, by yeast one-hybrid screening with the hatching enzyme promoter.
AuthorsWei Z, Angerer RC, Angerer LM
JournalMol Cell Biol
PubMed ID9891061
'We report the use of a yeast one-hybrid system to isolate a transcriptional regulator of the sea urchin embryo hatching enzyme gene, SpHE. This gene is asymmetrically expressed along the animal-vegetal axis of sea urchin embryos under the cell-autonomous control of maternal regulatory activities and therefore provides an excellent entry ... More
The retroviral oncogene qin belongs to the transcription factor family that includes the homeotic gene fork head.
AuthorsLi J, Vogt PK
JournalProc Natl Acad Sci U S A
PubMed ID8099441
Avian sarcoma virus 31 contains an oncogene that we have named qin. qin codes for a nuclear protein, Qin, that is a member of the HNF-3/fork head family of transcriptional regulators. Within this family Qin is particularly closely related to rat brain factor 1 (BF-1), a telencephalon-specific gene presumed to ... More
Exon Shuffling by L1 Retrotransposition
AuthorsJohn V. Moran, * Ralph J. DeBerardinis, Haig H. Kazazian Jr.
JournalScience
PubMed ID10066175
Long interspersed nuclear elements (LINE-1s or L1s) are the mostabundant retrotransposons in the human genome, and they serve asmajor sources of reverse transcriptase activity. Engineered L1sretrotranspose at high frequency in cultured human cells. Here it isshown that L1s insert into transcribed genes and retrotransposesequences derived from their 3' flanks to ... More
Mapping signal transduction pathways by phage display.
AuthorsZozulya S, Lioubin M, Hill RJ, Abram C, Gishizky ML
JournalNat Biotechnol
PubMed ID10585717
Rapid identification of proteins that interact with a novel gene product is an important element of functional genomics. Here we describe a phage display-based technique for interaction screening of complex cDNA libraries using proteins or synthetic peptides as baits. Starting with the epidermal growth factor receptor (EGFR) cytoplasmic tail, we ... More
Identification of a novel member of mouse hsp70 family. Its association with cellular mortal phenotype.
AuthorsWadhwa R, Kaul SC, Ikawa Y, Sugimoto Y
JournalJ Biol Chem
PubMed ID8454632
A novel 66-kDa protein, named p66mot-1, is identified to be associated with cellular mortality by virtue of its presence in cytosolic fractions of serially passaged mouse embryonic fibroblasts (MEF) and the mortal hybrids obtained by the fusion of mortal (MEF) and immortal (MN48-1, derivative of NIH 3T3) cells. Immortal cells ... More
Sodium pump isozymes are differentially expressed in electrically dissimilar regions of colonic circular smooth muscle.
AuthorsBurke EP, Sanders KM, Horowitz B
JournalProc Natl Acad Sci U S A
PubMed ID1848700
Molecular analyses of Na,K-ATPase abundance and alpha-subunit isoform distribution were performed to determine whether pump expression varies at different points through the thickness of the circular layer of colonic smooth muscle. The mRNA and polypeptides of Na,K-ATPase alpha 1 and beta subunits were twice as abundant in the submucosal region ... More
Identification and preliminary characterization of a protein motif related to the zinc finger.
We have identified a protein motif, related to the zinc finger, which defines a newly discovered family of proteins. The motif was found in the sequence of the human RING1 gene, which is proximal to the major histocompatibility complex region on chromosome six. We propose naming this motif the RING ... More
Characterization of molecular defects in xeroderma pigmentosum group C.
AuthorsLi L, Bales ES, Peterson CA, Legerski RJ
JournalNat Genet
PubMed ID8298653
Xeroderma pigmentosum (XP) is a rare autosomal recessive disease of humans characterized by an accelerated chronic degeneration of sun- exposed areas of the body, including an elevated risk of developing cancers of the skin. We recently reported the isolation of a gene XPCC that complements the repair deficiency of cultured ... More
Rearrangement of the PAX3 paired box gene in the paediatric solid tumour alveolar rhabdomyosarcoma.
We have determined that PAX3 (found previously to be mutated in Waardenburg syndrome) is the chromosome 2 locus rearranged by the t(2;13)(q35;q14) translocation of the paediatric solid tumour alveolar rhabdomyosarcoma. The rearrangement breakpoints occur within an intron downstream of the paired box and homeodomain-encoding regions. Upstream PAX3 sequences hybridize to ... More
RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs.
AuthorsEbinu JO, Bottorff DA, Chan EY, Stang SL, Dunn RJ, Stone JC
JournalScience
PubMed ID9582122
RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of EF hands that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling ... More
Loss of imprinting of IGF2 is linked to reduced expression and abnormal methylation of H19 in Wilms' tumour [published erratum appears in Nat Genet 1994 Oct;8(2):203]
AuthorsSteenman MJ, Rainier S, Dobry CJ, Grundy P, Horon IL, Feinberg AP
JournalNat Genet
PubMed ID7920665
The insulin-like growth factor-II (IGF2) and H19 genes are imprinted in mouse and human, with expression of the paternal IGF2 and maternal H19 alleles. IGF2 undergoes loss of imprinting (LOI) in most Wilms' tumours (WT). We now show that: (i) LOI of IGF2 is associated with a 80-fold down regulation ... More
Identification of four murine cDNAs encoding putative protein kinases from primitive embryonic stem cells differentiated in vitro.
AuthorsBiesecker LG, Gottschalk LR, Emerson SG
JournalProc Natl Acad Sci U S A
PubMed ID8346215
Protein kinases transduce signals from extracellular ligands in the hematopoietic and other systems through direct phosphorylation of tyrosine, serine, or threonine residues. Little is known about the ligands and receptors that are important in the earliest stages of development--i.e., stem cell self-renewal and lineage commitment. We have made use of ... More
Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma [published erratum appears in Nat Genet 1994 Feb;6(2):214]
AuthorsGalili N, Davis RJ, Fredericks WJ, Mukhopadhyay S, Rauscher FJ 3d, Emanuel BS, Rovera G, Barr FG
JournalNat Genet
PubMed ID8275086
We have examined the structure and expression of the products associated with the t(2;13)(q35;q14) translocation associated with alveolar rhabdomyosarcoma. The chromosome 13 gene (FKHR) is identified as a member of the fork head domain family of transcription factors characterized by a conserved DNA binding motif. Polymerase chain reaction analysis demonstrates ... More
Turning of retinal growth cones in a netrin-1 gradient mediated by the netrin receptor DCC.
Authorsde la Torre JR, Hopker VH, Ming GL, Poo MM, Tessier-Lavigne M, Hemmati-Brivanlou A, Holt CE
JournalNeuron
PubMed ID9427245
Netrin-1 promotes outgrowth of axons in vitro through the receptor Deleted in Colorectal Cancer (DCC) and elicits turning of axons within embryonic explants when presented as a point source. It is not known whether netrin-1 alone can elicit turning nor whether DCC mediates the turning response. We show that Xenopus ... More
CNS stem cells express a new class of intermediate filament protein.
AuthorsLendahl U, Zimmerman LB, McKay RD
JournalCell
PubMed ID1689217
Multipotential CNS stem cells receive and implement instructions governing differentiation to diverse neuronal and glial fates. Exploration of the mechanisms generating the many cell types of the brain depends crucially on markers identifying the stem cell state. We describe a gene whose expression distinguishes the stem cells from the more ... More
Lymphotactin: a cytokine that represents a new class of chemokine.
AuthorsKelner GS, Kennedy J, Bacon KB, Kleyensteuber S, Largaespada DA, Jenkins NA, Copeland NG, Bazan JF, Moore KW, Schall TJ, et al
JournalScience
PubMed ID7973732
In this study, the cytokine-producing profile of progenitor T cells (pro-T cells) was determined. During screening of a complementary DNA library generated from activated mouse pro-T cells, a cytokine designated lymphotactin was discovered. Lymphotactin is similar to members of both the Cys-Cys and Cys-X-Cys chemokine families but lacks two of ... More
Genetic differences in the ethanol sensitivity of GABAA receptors expressed in Xenopus oocytes.
AuthorsWafford KA, Burnett DM, Dunwiddie TV, Harris RA
JournalScience
PubMed ID1695761
Animal lines selected for differences in drug sensitivity can be used to help determine the molecular basis of drug action. Long-sleep (LS) and short-sleep (SS) mice differ markedly in their genetic sensitivity to ethanol. To investigate the molecular basis for this difference, mRNA from brains of LS and SS mice ... More
Expression of glutamate receptor genes in white matter: developing and adult rat optic nerve.
AuthorsJensen AM, Chiu SY
JournalJ Neurosci
PubMed ID8463842
We have identified the glutamate receptor genes expressed by developing and adult rat optic nerve. Results from PCR suggested that of the ionotropic glutamate receptor (GluR) gene family, only GluR1 and GluR3 subunits are expressed by optic nerve. However, Northern blot analysis demonstrated that only the GluR1 subtype is expressed ... More
Direct amplification of a single dissected chromosomal segment by polymerase chain reaction: a human brain sodium channel gene is on chromosome 2q22-q23.
AuthorsHan JA, Lu CM, Brown GB, Rado TA
JournalProc Natl Acad Sci U S A
PubMed ID1846440
We have devised a general strategy for gene mapping based upon the direct amplification of a target sequence within a single microdissected Giemsa-banded chromosomal segment using the polymerase chain reaction. The usefulness of this approach was demonstrated by mapping a cloned human brain sodium channel (alpha subunit) gene sequence to ... More
The mouse fidgetin gene defines a new role for AAA family proteins in mammalian development
AuthorsGregory A. Cox*, Connie L. Mahaffey*, Arne Nystuen, Verity A. Letts & Wayne N. Frankel *These authors contributed equally to this work.
JournalNat Genet
PubMed ID11017077
The mouse mutation fidget arose spontaneously in a heteroge-neousalbino stock 1 . This mutant mouse is characterized by aside-to-side head-shaking and circling behaviour, due to reducedor absent semicircular canals 2 . Fidget mice also have small eyes,associated with cell-cycle delay and insufficient growth of theretinal neural epithelium 3,4 , and ... More
Expression of members of the putative olfactory receptor gene family in mammalian germ cells.
AuthorsParmentier M, Libert F, Schurmans S, Schiffmann S, Lefort A, Eggerickx D, Ledent C, Mollereau C, Gerard C, Perret J, et al
JournalNature
PubMed ID1370859
A series of genomic and complementary DNA clones encoding new putative members of G protein-coupled receptors were isolated using homology cloning and low-stringency polymerase chain reaction. Among the unidentified receptors ('orphan receptors'), a human genomic clone (HGMP07) was characterized by the presence of its transcripts in the testis and by ... More
Regulation of 3beta-hydroxysteroid dehydrogenase in gonadotropin- induced steroidogenic desensitization of Leydig cells.
AuthorsTang PZ, Tsai-Morris CH, Dufau ML
JournalEndocrinology
PubMed ID9794458
3Beta-hydroxysteroid dehydrogenase/delta5-delta4 isomerases (3beta-HSD) are enzymes that catalyze the conversion of delta5 to delta4 steroids in the gonads and adrenal for the biosynthesis of sex steroid and corticoids. In gonadotropin-desensitized Leydig cells, from rats treated with high doses of human CG (hCG), testosterone production is markedly reduced, a finding that ... More
Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex.
AuthorsBastos R, Ribas de Pouplana L, Enarson M, Bodoor K, Burke B
JournalJ Cell Biol
PubMed ID9166401
The short filaments extending from the cytoplasmic face of nuclear pore complexes are thought to contain docking sites for nuclear import substrates. One component of these filaments is the large O-linked glycoprotein CAN/Nup214. Immunoprecipitation studies carried out under nondenaturing conditions, and using a variety of antibodies, reveal a novel nonglycosylated ... More
Macrophage-derived metalloelastase is responsible for the generation of angiostatin in Lewis lung carcinoma.
AuthorsDong Z, Kumar R, Yang X, Fidler IJ
JournalCell
PubMed ID9118223
To determine the mechanism responsible for the in vivo production of angiostatin that inhibits growth and metastasis in Lewis lung carcinoma (3LL), we implanted 3LL variant cells into the subcutis of syngeneic C57BL/6 mice. The tumors were infiltrated by macrophages and expressed high levels of steady-state mRNA for metalloelastase (MME). ... More
Differential response to RNA trans-splicing signals within the phosphoglycerate kinase gene cluster in Trypanosoma brucei.
AuthorsKapotas N, Bellofatto V
JournalNucleic Acids Res
PubMed ID8371981
In trypanosomatids, nuclear pre-mRNA splicing is exclusively a trans- splicing reaction in which a capped, 39 nt exon, the mini-exon, is positioned 5' to an open reading frame. Differential RNA splicing might reflect specific mini-exon and 3' splice site interactions. To test this hypothesis, we compared the efficiency of mini-exon ... More
Modulation of inhibitory glycine receptors by phosphorylation by protein kinase C and cAMP-dependent protein kinase.
AuthorsVaello ML, Ruiz-Gomez A, Lerma J, Mayor F Jr
JournalJ Biol Chem
PubMed ID8294452
Recent evidence has suggested a role for phosphorylation in the regulation of ligand-gated ion channels. We have recently shown (Ruiz- Gomez, A., Vaello, M., Valdivieso, F., and Mayor, F., Jr. (1991) J. Biol. Chem. 266, 559-566) that the inhibitory glycine receptor (GlyR) alpha subunit is phosphorylated in vitro by protein ... More
The 3;21 translocation in myelodysplasia results in a fusion transcript between the AML1 gene and the gene for EAP, a highly conserved protein associated with the Epstein-Barr virus small RNA EBER 1.
AuthorsNucifora G, Begy CR, Erickson P, Drabkin HA, Rowley JD
JournalProc Natl Acad Sci U S A
PubMed ID8395054
In the 8;21 translocation, the AML1 gene, located at chromosome band 21q22, is translocated to chromosome 8 (q22), where it is fused to the ETO gene and transcribed as a chimeric gene. AML1 is the human homolog of the recently cloned mouse gene pebp2 alpha B, homologous to the DNA ... More
The mouse antiphosphotyrosine immunoreactive kinase, TIK, is indistinguishable from the double-stranded RNA-dependent, interferon- induced protein kinase, PKR.
AuthorsBaier LJ, Shors T, Shors ST, Jacobs BL
JournalNucleic Acids Res
PubMed ID7694235
The mouse TIK protein, a serine/threonine kinase, was originally isolated from a murine pre-B cell expression library by its ability to bind anti-phosphotyrosine antibodies (Icely et al., J. Biol. Chem. 266, 16073-16077, 1991). The 67 kDa protein was found to have an associated autophosphorylation activity when incubated with ATP. Our ... More
A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4.
AuthorsWeinstein J, Jacobsen FW, Hsu-Chen J, Wu T, Baum LG
JournalMol Cell Biol
PubMed ID7513050
A novel protein, p55CDC, has been identified in cycling mammalian cells. This transcript is readily detectable in all exponentially growing cell lines but disappears when cells are chemically induced to fall out of the cell cycle and differentiate. The p55CDC protein appears to be essential for cell division, since transfection ... More
A universal method for the direct cloning of PCR amplified nucleic acid.
AuthorsMead DA, Pey NK, Herrnstadt C, Marcil RA, Smith LM
JournalBiotechnology (N Y)
PubMed ID1367662
We have devised a simple, universal cloning strategy that permits the direct ligation of PCR amplified nucleic acid to a compatible vector preparation. The method does not require that special restriction sites or additional sequences be appended onto the amplification primers, nor the use of restriction endonucleases, modifying enzymes, or ... More
Lack of significant differences in association rates and affinities of antibodies from short-term and long-term responses to hen egg lysozyme.
AuthorsGoldbaum FA, Cauerhff A, Velikovsky CA, Llera AS, Riottot MM, Poljak RJ
JournalJ Immunol
PubMed ID10229844
The affinities (Ka) and association rate constants (kon) of 23 mouse (BALB/c) anti-lysozyme mAbs obtained after short and prolonged immunizations have been measured by plasmon resonance techniques. The affinities for the 23 Abs, measured using their Fab, range from Ka = 1.1 x 10(7) to 1.4 x 10(10) M-1. There ... More
Purification, characterization, and cDNA cloning of alpha-N-acetylgalactosamine-specific lectin from starfish, Asterina pectinifera.
AuthorsKakiuchi M, Okino N, Sueyoshi N, Ichinose S, Omori A, Kawabata S, Yamaguchi K, Ito M,
JournalGlycobiology
PubMed ID11886841
We report here the purification, characterization, and cDNA cloning of a novel N-acetylgalactosamine-specific lectin from starfish, Asterina pectinifera. The purified lectin showed 19-kDa, 41-kDa, and 60-kDa protein bands on SDS-PAGE, possibly corresponding to a monomer, homodimer, and homotrimer. Interestingly, on 4-20% native PAGE the lectin showed at least nine protein ... More
Complex functional interactions at the early enhancer of the PQ strain of BK virus.
AuthorsFerguson AT, Subramani S
JournalJ Virol
PubMed ID8207802
BK virus is a human papovavirus that latently infects a majority of the world's population. There are more than 30 strains of the virus, most of which differ in the structure of the early enhancer region. The enhancer of the progenitor strain, WW, from which the other strains can be ... More
Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas.
AuthorsBaron BW, Nucifora G, McCabe N, Espinosa R 3d, Le Beau MM, McKeithan TW
JournalProc Natl Acad Sci U S A
PubMed ID8506375
Chromosomal translocations involving chromosome 3, band q27, are among the most common rearrangements in B-cell non-Hodgkin lymphoma. From a bacteriophage lambda library prepared from a lymphoma characterized by a t(3;14)(q27;q32), genomic clones were isolated using a probe from the immunoglobulin heavy chain locus (IGH) joining region. In addition to clones ... More
Molecular cloning and tissue distribution of keratocan. Bovine corneal keratan sulfate proteoglycan 37A.
Previous studies showed that the keratan sulfate-containing proteoglycans of bovine corneal stroma contain three unique core proteins designated 37A, 37B, and 25 (Funderburgh, J. L., Funderburgh, M. L., Mann, M. M., and Conrad, G. W. (1991) J. Biol. Chem. 266, 14226- 14231). Degenerate oligonucleotides designed from amino acid sequences of ... More
A testis-specific gene encoding a nuclear high-mobility-group box protein located in elongating spermatids.
AuthorsBoissonneault G, Lau YF
JournalMol Cell Biol
PubMed ID8321234
A cDNA encoding a DNA-binding protein has been isolated by screening a mouse testicular expression cDNA library with a concatemer of a 12-bp putative protein-binding element present in the promoter of the testis- specific gene PGK-2. Sequence analysis of the isolated cDNA indicated the presence of an open reading frame ... More
Cloning and characterization of complementary DNA encoding the eukaryotic initiation factor 2-associated 67-kDa protein (p67).
AuthorsWu S, Gupta S, Chatterjee N, Hileman RE, Kinzy TG, Denslow ND, Merrick WC, Chakrabarti D, Osterman JC, Gupta NK
JournalJ Biol Chem
PubMed ID8496145
The eukaryotic initiation factor 2 (eIF-2)-associated 67-kDa glycoprotein (p67) protects eIF-2 alpha-subunit from inhibitory phosphorylation by eIF-2 kinases, such as heme-regulated inhibitor and double-stranded RNA-activated inhibitor. This promotes protein synthesis in the presence of eIF-2 kinases present in animal cells (Ray, M. K., Datta, B., Chakraborty, A., Chattopadhyay, A., Meza- ... More
PU.1 is essential for p47(phox) promoter activity in myeloid cells.
AuthorsLi SL, Valente AJ, Zhao SJ, Clark RA
JournalJ Biol Chem
PubMed ID9211934
Expression of the phagocyte cytosolic protein p47(phox), a component of NADPH oxidase, is restricted mainly to myeloid cells. To study the cis- elements and trans-acting factors responsible for its gene expression, we have cloned and characterized the p47(phox) promoter. A predominant transcriptional start site was identified 21 nucleotides upstream of ... More
Knockout of the vesicular monoamine transporter 2 gene results in neonatal death and supersensitivity to cocaine and amphetamine.
AuthorsWang YM, Gainetdinov RR, Fumagalli F, Xu F, Jones SR, Bock CB, Miller GW, Wightman RM, Caron MG
JournalNeuron
PubMed ID9427251
Vesicular monoamine transporters are known to transport monoamines from the cytoplasm into secretory vesicles. We have used homologous recombination to generate mutant mice lacking the vesicular monoamine transporter 2 (VMAT2), the predominant form expressed in the brain. Newborn homozygotes die within a few days after birth, manifesting severely impaired monoamine ... More
Expression cloning of a cDNA for human leukotriene C4 synthase, an integral membrane protein conjugating reduced glutathione to leukotriene A4.
AuthorsLam BK, Penrose JF, Freeman GJ, Austen KF
JournalProc Natl Acad Sci U S A
PubMed ID8052639
Leukotriene (LT) C4 synthase, an integral microsomal membrane protein, conjugates LTA4, an epoxide intermediate, to reduced glutathione (GSH) to form a proinflammatory mediator, LTC4. A sensitive fluorescence- linked immunoassay for LTC4 was used to screen a KG-1 cDNA expression library for LTC4 synthase activity after transfection of COS cells and ... More
Otx1 and Otx2 define layers and regions in developing cerebral cortex and cerebellum.
AuthorsFrantz GD, Weimann JM, Levin ME, McConnell SK
JournalJ Neurosci
PubMed ID7931541
Within the cerebral and cerebellar cortices, neurons are organized in layers that segregate neurons with distinctive morphologies and axonal connections, and areas or regions that correspond to distinct functional domains. To explore the molecular underpinnings of pattern formation in layered regions of the CNS, we have characterized the patterns of ... More
An overexpressed gene transcript in senescent and quiescent human fibroblasts encoding a novel protein in the epidermal growth factor- like repeat family stimulates DNA synthesis.
AuthorsLecka-Czernik B, Lumpkin CK Jr, Goldstein S
JournalMol Cell Biol
PubMed ID7799918
We carried out subtractive enrichment of a cDNA library derived from mRNA of senescent human diploid fibroblasts (HDF) established from a subject with Werner syndrome of premature aging. By differential screening, we isolated an overexpressed cDNA sequence (S1-5) that codes for a novel protein containing epidermal growth factor (EGF)-like domains ... More
Alternative promoter usage and splicing options result in the differential expression of mRNAs encoding four isoforms of chicken VBP, a member of the PAR subfamily of bZIP transcription factors.
AuthorsBurch JB, Davis DL
JournalNucleic Acids Res
PubMed ID7984425
We previously isolated a set of overlapping cDNA clones that encoded a unique open reading frame for the chicken VBP transcription factor. We now report the isolation of a cDNA clone that encodes a complete open reading frame for a VBP isoform that differs from the previously reported sequence at ... More
Antimitogenic and mitogenic actions of interleukin-1 in diverse cell types are associated with induction of gro gene expression.
AuthorsRangnekar VV, Waheed S, Davies TJ, Toback FG, Rangnekar VM
JournalJ Biol Chem
PubMed ID1989993
Interleukin-1 (IL-1) is an antiproliferative factor for growing human melanoma A375-C6 cells. To define the molecular basis for the action of IL-1, we set out to identify early genes induced by the cytokine in the absence of de novo protein synthesis. cDNA libraries were constructed from A375-C6 cells that were ... More
The genomic organization of the region containing the Drosophila melanogaster rpL7a (Surf-3) gene differs from those of the mammalian and avian Surfeit loci.
AuthorsArmes N, Fried M
JournalMol Cell Biol
PubMed ID7739520
The Surf-3 gene of the unusually tight mouse Surfeit locus gene cluster has been identified as the highly conserved ribosomal protein gene L7a (rpL7a). The topography and juxtaposition of the Surfeit locus genes are conserved for the 600 million years of divergent evolution between mammals and birds. This suggests cis ... More
Cloning and expression of the cDNA encoding the human homologue of the DNA repair enzyme, Escherichia coli endonuclease III.
We previously purified a bovine pyrimidine hydrate-thymine glycol DNA glycosylase/AP lyase. The amino acid sequence of tryptic bovine peptides was homologous to Escherichia coli endonuclease III, theoretical proteins of Saccharomyces cerevisiae and Caenorhabditis elegans, and the translated sequences of rat and human 3'-expressed sequence tags (3'-ESTs) (Hilbert, T. P., Boorstein, ... More
Characterization of a 4.8kb transcript from the Duchenne muscular dystrophy locus expressed in Schwannoma cells.
AuthorsBlake DJ, Love DR, Tinsley J, Morris GE, Turley H, Gatter K, Dickson G, Edwards YH, Davies KE
JournalHum Mol Genet
PubMed ID1301145
The 14kb dystrophin transcript from the Duchenne muscular dystrophy (DMD) locus, which encodes a 427kDa protein, is differentially spliced at the amino terminal end giving rise to alternative transcripts expressed in muscle and brain. Here we present evidence for a 4.8kb transcript from the DMD locus which is ubiquitously expressed ... More
A novel transcription factor regulates expression of the vacuolar H+- ATPase B2 subunit through AP-2 sites during monocytic differentiation.
AuthorsLee BS, Krits I, Crane-Zelkovic MK, Gluck SL
JournalJ Biol Chem
PubMed ID8995244
During monocyte-to-macrophage differentiation, the cellular content of vacuolar H+-ATPase (V-ATPase) increases more than 4-fold. We have shown previously that amplified expression of the B2 subunit of the V-ATPase occurs solely by increased transcription, and that the 5'-untranslated region of the B2 gene, containing multiple consensus binding sites for the transcription ... More
Differential accumulation of transcripts encoding protein kinase homologs in greening pea seedlings.
AuthorsLin X, Feng XH, Watson JC
JournalProc Natl Acad Sci U S A
PubMed ID1714582
Degenerate oligonucleotides, corresponding to conserved regions within the catalytic domain of known protein-serine/threonine kinases, were used as primers for the polymerase chain reaction to amplify cDNA synthesized from poly(A)+ RNA purified from the apical buds of 7-day- old pea seedlings. Five partial cDNAs were obtained and designated PsPK1 through PsPK5 ... More
Complement inhibitor of C5 activation from the soft tick Ornithodoros moubata.
AuthorsNunn MA, Sharma A, Paesen GC, Adamson S, Lissina O, Willis AC, Nuttall PA,
JournalJ Immunol
PubMed ID15699138
Blood-feeding ticks must control C activation or be damaged by the host inflammatory response. We report the characterization and expression of a novel, relatively small, broad-acting C inhibitory protein (termed OmCI) from the soft tick Ornithodoros moubata. The native 17-kDa nonglycosylated protein inhibits both human and guinea pig classical and ... More
Characterization of anti-anti-idiotypic antibodies that bind antigen and an anti-idiotype.
AuthorsGoldbaum FA, Velikovsky CA, Dall'Acqua W, Fossati CA, Fields BA, Braden BC, Poljak RJ, Mariuzza RA
JournalProc Natl Acad Sci U S A
PubMed ID9238040
Two mouse monoclonal anti-anti-idiotopic antibodies (anti-anti-Id, Ab3), AF14 and AF52, were prepared by immunizing BALB/c mice with rabbit polyclonal anti-idiotypic antibodies (anti-Id, Ab2) raised against antibody D1.3 (Ab1) specific for the antigen hen egg lysozyme. AF14 and AF52 react with an internal image monoclonal mouse anti-Id antibody E5.2 (Ab2), previously ... More
Cytoplasmic dynein plays a role in mammalian mitotic spindle formation.
AuthorsVaisberg EA, Koonce MP, McIntosh JR
JournalJ Cell Biol
PubMed ID8227145
The formation and functioning of a mitotic spindle depends not only on the assembly/disassembly of microtubules but also on the action of motor enzymes. Cytoplasmic dynein has been localized to spindles, but whether or how it functions in mitotic processes is not yet known. We have cloned and expressed DNA ... More
Loss of E-cadherin-dependent cell-cell adhesion due to mutation of the beta-catenin gene in a human cancer cell line, HSC-39.
AuthorsKawanishi J, Kato J, Sasaki K, Fujii S, Watanabe N, Niitsu Y
JournalMol Cell Biol
PubMed ID7862112
Detachment of cell-cell adhesion is indispensable for the first step of invasion and metastasis of cancer. This mechanism is frequently associated with the impairment of either E-cadherin expression or function. However, mechanisms of such abnormalities have not been fully elucidated. In this study, we demonstrated that the function of E- ... More
Cloning and characterization of chicken YB-1: regulation of expression in the liver.
AuthorsGrant CE, Deeley RG
JournalMol Cell Biol
PubMed ID8321222
A cDNA expression library constructed from day 9 embryonic liver was screened with a previously identified protein binding site in the flanking region of the liver-specific, estrogen-dependent avian apoVLDLII gene. Two of the clones isolated were shown to encode the chicken homolog of the Y-box binding protein, YB-1 (dbpb), which ... More
In situ detection of starvation-induced autophagy.
AuthorsMartinet W, De Meyer GR, Andries L, Herman AG, Kockx MM,
JournalJ Histochem Cytochem
PubMed ID16148314
Autophagy is a regulated bulk degradation process involved in many different human pathologies. Transmission electron microscopy (TEM) is currently the only reliable method for monitoring autophagy in situ. Because TEM is labor intensive, we questioned whether useful marker proteins can be found for unambiguous detection of autophagy in tissue via ... More
Characterization of an orphan G protein-coupled receptor localized in the dorsal root ganglia reveals adenine as a signaling molecule.
Authors Bender Eckhard; Buist Arjan; Jurzak Mirek; Langlois Xavier; Baggerman Geert; Verhasselt Peter; Ercken Martine; Guo Hong-Qing; Wintmolders Cindy; Van den Wyngaert Ilse; Van Oers Irma; Schoofs Liliane; Luyten Walter;
JournalProc Natl Acad Sci U S A
PubMed ID12084918
The cloning of novel G protein-coupled receptors and the search for their natural ligands, a process called reverse pharmacology, is an excellent opportunity to discover novel hormones and neurotransmitters. Based on a degenerate primer approach we have cloned a G protein-coupled receptor whose mRNA expression profile indicates highest expression in ... More
Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.
Authors Han J; Flemington C; Houghton A B; Gu Z; Zambetti G P; Lutz R J; Zhu L; Chittenden T;
JournalProc Natl Acad Sci U S A
PubMed ID11572983
BH3-only proteins function at a proximal point in a conserved cell death pathway by binding, through their BH3 domains, to other Bcl-2 family members and triggering mitochondrial events associated with apoptosis. Here, we describe a strongly pro-apoptotic BH3-only protein, designated Bbc3, whose expression increases in response to diverse apoptotic stimuli. ... More
Expression of functional neurotransmitter receptors in Xenopus oocytes after injection of human brain membranes.
The Xenopus oocyte is a very powerful tool for studies of the structure and function of membrane proteins, e.g., messenger RNA extracted from the brain and injected into oocytes leads to the synthesis and membrane incorporation of many types of functional receptors and ion channels, and membrane vesicles from Torpedo ... More
Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications.
Authors Sorlie T; Perou C M; Tibshirani R; Aas T; Geisler S; Johnsen H; Hastie T; Eisen M B; van de Rijn M; Jeffrey S S; Thorsen T; Quist H; Matese J C; Brown P O; Botstein D; Eystein Lønning P; Børresen-Dale A L;
JournalProc Natl Acad Sci U S A
PubMed ID11553815
The purpose of this study was to classify breast carcinomas based on variations in gene expression patterns derived from cDNA microarrays and to correlate tumor characteristics to clinical outcome. A total of 85 cDNA microarray experiments representing 78 cancers, three fibroadenomas, and four normal breast tissues were analyzed by hierarchical ... More
The KH-type RNA-binding protein PSI is required for Drosophila viability, male fertility, and cellular mRNA processing.
Authors Labourier Emmanuel; Blanchette Marco; Feiger Jennie W; Adams Melissa D; Rio Donald C;
JournalGenes Dev
PubMed ID11782446
Direct interactions between RNA-binding proteins and snRNP particles modulate eukaryotic pre-mRNA processing patterns to control gene expression. Here, we report that the conserved U1 snRNP-interacting RNA-binding protein PSI is essential for Drosophila viability. A null PSI mutation is recessive lethal at the first-instar larval stage, and lethality is fully rescued ... More
A Subtractive Gene Expression Screen Suggests a Role of Transcription Factor AP-2alpha in Control of Proliferation and Differentiation.
The transcription factor AP-2α has been implicated as a cell type-specific regulator of gene expression during vertebrate embryogenesis based on its expression pattern in neural crest cells, ectoderm, and the nervous system in mouse and frog embryos. AP-2α is prominently expressed in cranial neural crest cells, a population of cells ... More
The macrophage C-type lectin specific for galactose/N-acetylgalactosamine is an endocytic receptor expressed on monocyte-derived immature dendritic cells.
Lectins on antigen presenting cells are potentially involved in the antigen uptake and the cellular recognition and trafficking. Serial analysis of gene expression in monocyte-derived dendritic cells (DCs), monocytes, and macrophages revealed that 7 of the 19 C-type lectin mRNA were present in immature DCs. Two of these, the macrophage ... More