Screening procedure for Pichia pastoris clones containing multiple copy gene inserts [In Process Citation]
AuthorsFairlie WD, Russell PK, Zhang HP, Breit SN
JournalBiotechniques
PubMed ID10376135
Heteropentameric cholera toxin B subunit chimeric molecules genetically fused to a vaccine antigen induce systemic and mucosal immune responses: a potential new strategy to target recombinant vaccine antigens to mucosal immune systems.
AuthorsHarakuni T, Sugawa H, Komesu A, Tadano M, Arakawa T,
JournalInfect Immun
PubMed ID16113283
Noninvasive mucosal vaccines are attractive alternatives to parenteral vaccines. Although the conjugation of vaccine antigens with the B subunit of cholera toxin (CTB) is one of the most promising strategies for vaccine delivery to mucosal immune systems, the molecule cannot tolerate large-protein fusion, as it severely impairs pentamerization and loses ... More