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View additional product information for S.N.A.P.™ Plasmid DNA MiniPrep Kit - FAQs (K190001)
8 product FAQs found
Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.
The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.
The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.
The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.
The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.
Sometimes there is insoluble material (probably resin fines) in the precipitated DNA. This material is very easily removed with a 10 second spin. The presence of these particles does not reflect a poor preparation. Once the particles are removed, the DNA performs well in all applications.
Please visit our Plasmid DNA Purification page for the most up-to-date information:
http://www.thermofisher.com/us/en/home/life-science/DNA-RNA-Purification-Analysis/DNA-Purification/Plasmid-DNA-Purification.html
No, we know of no clinical centrifuges that can reach 5,000 x g. Most clinical centrifuges have a maximum in the range of 3,000 to 4,000 x g. A high-speed swinging bucket rotor is therefore required for these systems. Often researchers will attempt to compensate for lower g force by increasing centrifugation time; however, this will not work.
We have many products suitable for Miniprep and Midiprep purifications:
The PureLink HiPure Plasmid Miniprep and Midiprep purification kits are based on anion-exchange technology and allow high-yield isolation of highly pure plasmid DNA, equivalent to two passes through CsCl (cesium chloride) gradients. Plasmid isolated with these kits will have the lowest endotoxin levels and will work best for difficult transfections and other challenging applications.
The PureLink HQ Mini Plasmid DNA Purification Kit is a modified silica technology designed to yield high amounts of high-quality plasmid DNA for use in standard mammalian cell transfections, restriction enzyme digestion, PCR, automated sequencing, and transformation. This kit is also available in 96-well format.
The PureLink Quick Plasmid Miniprep Kit is a silica-based purification kit designed to isolate high-quality plasmid DNA suitable for sequencing and cloning applications in 25-45 minutes.
The ChargeSwitch-Pro Plasmid Miniprep Kit uses our ChargeSwitch technology where plasmid is bound to a surface ligand that is positively charged at low pH, and eluted when the pH is raised to 8.5. The ChargeSwitch protocol retains the ease and familiarity of common silica spin-column protocols with binding, washing, and elution steps. Since this method does not require the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification, the resulting DNA is free of potentially inhibitory compounds.
The ChargeSwitch Plasmid ER and Plasmid Yeast Mini kits use our ChargeSwitch magnetic bead-based technology for isolation of high-quality plasmid DNA.
The ChargeSwitch NoSpin Plasmid Micro kit is a novel magnetic bead technology that eliminates the need for centrifugation in the plasmid purification procedures.
The ChargeSwitch products are easily automated on robotic platforms.