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查看更多产品信息 S.N.A.P.™ Plasmid DNA MidiPrep Kit - FAQs (K191001)
6 个常见问题解答
The Sorval RT6000B table-top centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol. The low-speed spins are around 2,000 rpm and the high-speed spins are around 3,000 rpm. You may also use the Sorval RC-5B centrifuge with the SS-34 rotor. However, you might observe some spillage with this centrifuge.
Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.
The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.
The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.
The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.
The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.
No, we know of no clinical centrifuges that can reach 5,000 x g. Most clinical centrifuges have a maximum in the range of 3,000 to 4,000 x g. A high-speed swinging bucket rotor is therefore required for these systems. Often researchers will attempt to compensate for lower g force by increasing centrifugation time; however, this will not work.