S.N.A.P.™ UV-Free Gel Purification Kit - FAQs

查看更多产品信息 S.N.A.P.™ UV-Free Gel Purification Kit - FAQs (K200025)

6 个常见问题解答

我使用TAE或TBE缓冲体系来进行琼脂糖凝胶电泳，可以使用你们的凝胶提取试剂盒吗？

可以，我们的凝胶提取试剂盒和TAE及TBE琼脂糖凝胶均兼容。

I'm running an agarose gel with TAE or TBE. Can I use your gel extraction kits?

Yes, both TAE and TBE agarose gels are compatible with our gel extraction kits.

Can you use TBE gels with S.N.A.P. gel purification kits?

No, TBE gels contain borate, which interferes with the sodium iodide solution. Therefore, only TAE gels can be used. We recommend the PureLink Quick Gel Extraction kit for both TAE and TBE agarose gels.

What is the smallest fragment that can be purified with S.N.A.P. columns?

S.N.A.P. mini prep columns have been used to purify fragments of 100 bp in gel purification procedures.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

What is unique about the S.N.A.P. UV-free Gel Purification Kit?

The S.N.A.P. UV-free Gel Purification Kit utilizes crystal violet as a staining reagent, which can be visualized in non-UV light conditions. This can prevent DNA damage that can be caused by the UV light required by normal ethidium bromide staining. DNA fragments recovered using this kit have higher cloning efficiency. In addition, DNA can be visualized under normal light as a thin violet band while the gel is running and excised as soon as bands are sufficiently resolved.