'Cdc25 phosphatases are dual specificity phosphatases that dephosphorylate and activate cyclin-dependent kinases (CDKs), thereby effecting the progression from one phase of the cell cycle to the next. Despite its central role in the cell cycle, relatively little is known about the catalytic mechanism of Cdc25. In order to provide insights ... More
Cloning and functional characterization of a rat renal organic cation transporter isoform (rOCT1A).
AuthorsZhang L, Dresser MJ, Chun JK, Babbitt PC, Giacomini KM
JournalJ Biol Chem
PubMed ID9195965
'Polyspecific organic cation transporters in the renal proximal tubule mediate the secretion of many clinically used drugs as well as endogenous metabolites. Recently, two organic cation transporters (rOCT1 and rOCT2) were cloned from rat kidney. In this study, we report the cloning and functional expression of an rOCT1 isoform, rOCT1A, ... More
Differential gene expression between developing queens and workers in the honey bee, Apis mellifera [see comments]
AuthorsEvans JD, Wheeler DE
JournalProc Natl Acad Sci U S A
PubMed ID10318926
'Many insects show polyphenisms, or alternative morphologies, which are based on differential gene expression rather than genetic polymorphism. Queens and workers are alternative forms of the adult female honey bee and represent one of the best known examples of insect polyphenism. Hormonal regulation of caste determination in honey bees has ... More
Semaphorin III can function as a selective chemorepellent to pattern sensory projections in the spinal cord.
AuthorsMessersmith EK, Leonardo ED, Shatz CJ, Tessier-Lavigne M, Goodman CS, Kolodkin AL
JournalNeuron
PubMed ID7748562
'Distinct classes of primary sensory neurons in dorsal root ganglia subserve different sensory modalities, terminate in different dorsoventral locations in the spinal cord, and display different neurotrophin response profiles. Large diameter muscle afferents that terminate in the ventral spinal cord are NT-3 responsive, whereas small diameter afferents subserving pain and ... More
Nopp140 is a mediator of the protein kinase A signaling pathway that activates the acute phase response alpha1-acid glycoprotein gene.
Authors Chiu Chi-Ming; Tsay Yeou-Guang; Chang Ching-Jin; Lee Sheng-Chung;
JournalJ Biol Chem
PubMed ID12167624
'The acute phase response (APR) in liver during inflammation is one of the well known examples for elucidating the signaling pathways that lead to the combinatorial regulation of gene expression. The APR is exemplified by alpha(1)-acid glycoprotein gene (agp) expression. A number of transcription factors, including CCAAT/enhancer-binding protein beta (C/EBPbeta), ... More
Upstream organization of and multiple transcripts from the human folylpoly-gamma-glutamate synthetase gene.
AuthorsFreemantle SJ, Taylor SM, Krystal G, Moran RG
JournalJ Biol Chem
PubMed ID7721888
'Folylpoly-gamma-glutamate synthetase (FPGS) is essential for the survival of proliferating mammalian cells and central to the action of all classical folate antimetabolites. We report the isolation of cDNAs corresponding to the 5'' ends of FPGS mRNA from both human and hamster cells which include a start codon upstream of and ... More
CTCF mediates methylation-sensitive enhancer-blocking activity at the H19/Igf2 locus.
'The Insulin-like growth factor 2 (Igf2) and H19 genes are imprinted, resulting in silencing of the maternal and paternal alleles, respectively. This event is dependent upon an imprinted-control region two kilobases upstream of H19 (refs 1, 2). On the paternal chromosome this element is methylated and required for the silencing ... More
Excision of IS492 requires flanking target sequences and results incircle formation in Pseudoalteromonas atlantica.
AuthorsPerkins-Balding D, Duval-Valentin G, Glasgow AC
JournalJ Bacteriol
PubMed ID10438765
'The gram-negative marine bacterium Pseudoalteromonas atlantica produces extracellular polysaccharide (EPS) that isimportant in biofilm formation by this bacterium. Insertion and precise excision of IS492 at a locus essential forextracellular polysaccharide production (eps) controls phase variation of EPS production in P. atlantica. Examinationof IS492 transposition in P. atlantica by using a ... More
Liver-derived insulin-like growth factor I (IGF-I) is the principal source of IGF-I in blood but is not required for postnatal body growth in mice.
AuthorsSjogren K, Liu JL, Blad K, Skrtic S, Vidal O, Wallenius V, LeRoith D, Tornell J, Isaksson OG, Jansson JO, Ohlsson C
JournalProc Natl Acad Sci U S A
PubMed ID10359843
'The body growth of animals is regulated by growth hormone and IGF-I. The classical theory of this regulation is that most IGF-I in the blood originates in the liver and that body growth is controlled by the concentration of IGF-I in the blood. We have abolished IGF-I production in the ... More
General method for PCR amplification and direct sequencing of mRNA differential display products.
AuthorsReeves SA, Rubio MP, Louis DN
JournalBiotechniques
PubMed ID7702844
No abstract
Editing of a tRNA anticodon in marsupial mitochondria changes its codon recognition.
AuthorsJanke A, Paabo S
JournalNucleic Acids Res
PubMed ID8479901
RNA editing has been described for organellar genes of protozoa and plants as well as in three cases for nuclear transcripts of mammals. The mitochondrial tRNA for aspartic acid is encoded in the mitochondrial genome of marsupials with the anticodon GCC. However, cDNA from the gene product revealed that the ... More
TECK: a novel CC chemokine specifically expressed by thymic dendritic cells and potentially involved in T cell development.
A novel CC chemokine was identified in the thymus of mouse and human and was designated TECK (thymus-expressed chemokine). TECK has weak homology to other CC chemokines and maps to mouse chromosome 8. Besides the thymus, mRNA encoding TECK was detected at substantial levels in the small intestine and at ... More
Disruption of imprinting caused by deletion of the H19 gene region in mice [see comments]
AuthorsLeighton PA, Ingram RS, Eggenschwiler J, Efstratiadis A, Tilghman SM
JournalNature
PubMed ID7536897
The imprinted H19 gene, which encodes an untranslated RNA, lies at the end of a cluster of imprinted genes in the mouse. Imprinting of the insulin-2 and insulin-like growth factor 2 genes, which lie about 100 kilobases upstream of H19, can be disrupted by maternal inheritance of a targeted deletion ... More
Two period homologs: circadian expression and photic regulation in the suprachiasmatic nuclei.
We have characterized a mammalian homolog of the Drosophila period gene and designated it Per2. The PER2 protein shows >40% amino acid identity to the protein of another mammalian per homolog (designated Per1) that was recently cloned and characterized. Both PER1 and PER2 proteins share several regions of homology with ... More
timrit Lengthens circadian period in a temperature-dependent manner through suppression of PERIOD protein cycling and nuclear localization.
AuthorsMatsumoto A, Tomioka K, Chiba Y, Tanimura T
JournalMol Cell Biol
PubMed ID10330175
A fundamental feature of circadian clocks is temperature compensation of period. The free-running period of ritsu (timrit) (a novel allele of timeless [tim]) mutants is drastically lengthened in a temperature- dependent manner. PER and TIM protein levels become lower in timrit mutants as temperature becomes higher. This mutation reduces per ... More
A new dexamethasone-induced gene of the leucine zipper family protects T lymphocytes from TCR/CD3-activated cell death.
AuthorsD'Adamio F, Zollo O, Moraca R, Ayroldi E, Bruscoli S, Bartoli A, Cannarile L, Migliorati G, Riccardi C
JournalImmunity
PubMed ID9430225
By comparing mRNA species expressed in dexamethasone (DEX)-treated and untreated murine thymocytes, we have identified a gene, glucocorticoid- induced leucine zipper (GILZ), encoding a new member of the leucine zipper family. GILZ was found expressed in normal lymphocytes from thymus, spleen, and lymph nodes, whereas low or no expression was ... More
Kainate receptor subunits expressed in single cultured hippocampal neurons: molecular and functional variants by RNA editing.
To determine the kainate receptor subunits that are found in native kainate receptors, we have applied a multiplex PCR of cDNAs reverse transcribed from mRNA harvested from single cultured hippocampal neurons after electrophysiological recording. We found that all the cells showing rapidly desensitizing currents in response to kainate express the ... More
A role of Ultrabithorax in morphological differences between Drosophila species.
AuthorsStern DL
JournalNature
PubMed ID9853753
The mechanisms underlying the evolution of morphology are poorly understood. Distantly related taxa sometimes exhibit correlations between morphological differences and patterns of gene expression, but such comparisons cannot establish how mechanisms evolve to generate diverse morphologies. Answers to these questions require resolution of the nature of developmental evolution within and ... More
A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling.
AuthorsChang M, French-Cornay D, Fan HY, Klein H, Denis CL, Jaehning JA
JournalMol Cell Biol
PubMed ID9891041
Yeast contains at least two complex forms of RNA polymerase II (Pol II), one including the Srbps and a second biochemically distinct form defined by the presence of Paf1p and Cdc73p (X. Shi et al., Mol. Cell. Biol. 17:1160-1169, 1997). In this work we demonstrate that Ccr4p and Hpr1p are ... More
A Drosophila SH2-SH3 adaptor protein implicated in coupling the sevenless tyrosine kinase to an activator of Ras guanine nucleotide exchange, Sos.
AuthorsOlivier JP, Raabe T, Henkemeyer M, Dickson B, Mbamalu G, Margolis B, Schlessinger J, Hafen E, Pawson T
JournalCell
PubMed ID8462098
A Drosophila gene (drk) encodes a widely expressed protein with a single SH2 domain and two flanking SH3 domains, which is homologous to the Sem-5 protein of C. elegans and mammalian GRB2. Genetic analysis suggests that drk function is essential for signaling by the sevenless receptor tyrosine kinase. Drk biological ... More
Activation of transcription by binding of NF-E1 (YY1) to a newly identified element in the first exon of the human DR alpha gene.
AuthorsHehlgans T, Strominger JL
JournalJ Immunol
PubMed ID7730624
A previously unrecognized element, located downstream of the start site of transcription in the first exon of the DR alpha gene, has been defined that enhances promoter activity up to eightfold in a position- dependent manner. Mutations in this DNA-binding site abolished binding of a nuclear factor in human B ... More
Rearrangement and expression of the human psi C lambda 6 gene segment results in a surface Ig receptor with a truncated light chain constant region.
AuthorsStiernholm NB, Verkoczy LK, Berinstein NL
JournalJ Immunol
PubMed ID7722311
The constant region of the human Ig lambda locus consists of seven tandemly organized J-C gene segments. Although it has been established that the J-C lambda 1, J-C lambda 2, J-C lambda 3, and J-C lambda 7 gene segments are functional, and code for the four distinct Ig lambda isotypes ... More
Identification of a glucose response element in the promoter of the rat glucagon receptor gene.
AuthorsPortois L, Maget B, Tastenoy M, Perret J, Svoboda M
JournalJ Biol Chem
PubMed ID10075722
We cloned the 5' upstream region of the rat glucagon receptor gene, demonstrating that the 5' noncoding domain of the glucagon receptor mRNA contained two untranslated exons of 131 and 166 nucleotides (nt), respectively, separated by two introns of 0.6 and 3.2 kilobase pairs. We also observed an alternative splicing ... More
Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris.
AuthorsGuo W, Gonzalez-Candelas L, Kolattukudy PE
JournalJ Bacteriol
PubMed ID8522511
Since plant-pathogenic fungi must penetrate through pectinaceous layers of the host cell wall, pectin-degrading enzymes are thought to be important for pathogenesis. Antibodies prepared against a pectin- inducible pectate lyase (pectate lyase A [PLA]) produced by a phytopathogenic fungus, Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI), was ... More
Bipotential primitive-definitive hematopoietic progenitors in the vertebrate embryo.
AuthorsTurpen JB, Kelley CM, Mead PE, Zon LI
JournalImmunity
PubMed ID9324353
Two regions of the vertebrate embryo, the blood islands and the dorsal lateral plate (DLP), participate in early hematopoietic development. In Xenopus, primitive erythrocytes are derived solely from the ventral blood islands (VBI), while definitive hematopoietic cells such as lymphocytes are derived from both VBI and DLP. We have utilized ... More
Two widely spaced initiator binding sites create an HMG1-dependent parvovirus rolling-hairpinreplication origin.
AuthorsCotmore SF, Christensen J, Tattersall P
JournalJ Virol
PubMed ID10627544
Minute virus of mice (MVM) replicates via a linearized form of rolling-circle replication in which the viral nickase, NS1, initiates DNA synthesis by introducing a site-specific nick into either of two distinct origin sequences. In vitro nicking and replication assays with substrates that had deletions or mutations were used to ... More
Mutations in HYAL1, a member of a tandemly distributed multigene family encoding disparate hyaluronidase activities, cause a newly described lysosomal disorder, mucopolysaccharidosis IX.
AuthorsTriggs-Raine B, Salo TJ, Zhang H, Wicklow BA, Natowicz MR
JournalProc Natl Acad Sci U S A
PubMed ID10339581
Hyaluronan (HA), a large glycosaminoglycan abundant in the extracellular matrix, is important in cell migration during embryonic development, cellular proliferation, and differentiation and has a structural role in connective tissues. The turnover of HA requires endoglycosidic breakdown by lysosomal hyaluronidase, and a congenital deficiency of hyaluronidase has been thought to ... More
Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats.
AuthorsShanley TP, Schmal H, Friedl HP, Jones ML, Ward PA
JournalJ Immunol
PubMed ID7722328
The role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in the pathogenesis of acute lung injury in rats after intrapulmonary deposition of IgG immune complexes or intratracheal administration of LPS has been assessed. Critical to these studies was the cloning and functional expression of rat MIP-1 alpha. The resulting product ... More
Expression of a mutant human fibrillin allele upon a normal human or murine genetic background recapitulates a Marfan cellular phenotype.
AuthorsEldadah ZA, Brenn T, Furthmayr H, Dietz HC
JournalJ Clin Invest
PubMed ID7860770
The Marfan syndrome (MFS) is a connective tissue disorder inherited as an autosomal dominant trait and caused by mutations in the gene encoding fibrillin, a 350-kD glycoprotein that multimerizes to form extracellular microfibrils. It has been unclear whether disease results from a relative deficiency of wild-type fibrillin; from a dominant- ... More
DUB-2 is a member of a novel family of cytokine-inducible deubiquitinating enzymes.
AuthorsZhu Y, Lambert K, Corless C, Copeland NG, Gilbert DJ, Jenkins NA, D'Andrea AD
JournalJ Biol Chem
PubMed ID8995226
Cytokines regulate cell growth by inducing the expression of specific target genes. We have recently identified a cytokine-inducible, immediate-early gene, DUB-1, that encodes a deubiquitinating enzyme with growth regulatory activity. In the current study, we have isolated a highly related gene, DUB-2, that is induced by interleukin-2. The DUB- 2 ... More
Molecular cloning of a gene encoding a new type of metalloproteinase- disintegrin family protein with thrombospondin motifs as an inflammation associated gene.
AuthorsKuno K, Kanada N, Nakashima E, Fujiki F, Ichimura F, Matsushima K
JournalJ Biol Chem
PubMed ID8995297
A cellular disintegrin and metalloproteinase (ADAM) is a new family of genes with structural homology to the snake venom metalloproteinases and disintegrins. We screened genes which were selectively expressed in the cachexigenic colon 26 adenocarcinoma subline in vivo. It was found that one novel cDNA clone, identified as a cachexigenic ... More
Differential distribution of Ca2+-activated K+ channel splice variants among hair cells along the tonotopic axis of the chick cochlea.
AuthorsNavaratnam DS, Bell TJ, Tu TD, Cohen EL, Oberholtzer JC
JournalNeuron
PubMed ID9390520
We have cloned from the receptor epithelium of the chick cochlea a family of alternatively spliced cDNAs derived from cslo, which encodes a Ca2+-activated K+ channel like those shown to help determine the resonant frequency of electrically tuned hair cells. Our results from PCRs using template RNAs from both tonotopically ... More
A dominant T cell receptor beta-chain in response to a short ragweed allergen, Amb a 5.
AuthorsHuang SK, Yi M, Palmer E, Marsh DG
JournalJ Immunol
PubMed ID7751655
HLA-DR alpha, beta 1 *1501 (and the closely related DR alpha, beta 1 *1502) heterodimers are the primary class II MHC restriction elements controlling T cell responses to a ragweed pollen allergen, Amb a 5 (M(r) = 5000). Using a novel, quantitative, competitive PCR (QC-PCR) assay and a TCR beta-gene ... More
Regulation of the Murine Nfatc1 Gene by NFATc2.
Authors Zhou Bin; Cron Randy Q; Wu Bingruo; Genin Anna; Wang Zhili; Liu Steve; Robson Paul; Baldwin H Scott;
JournalJ Biol Chem
PubMed ID11786533
NFAT proteins play a key role in the inducible expression of cytokine genes in T lymphocytes. NFATc1 and NFATc2 are the predominant NFAT family members in the peripheral immune system. NFATc2 is found abundantly in the cytoplasm of resting T cells, whereas Nfatc1 expression is induced during T cell activation. ... More
ATPase activity of the terminase subunit pUL56 of human cytomegalovirus.
Authors Hwang Jae-Seon; Bogner Elke;
JournalJ Biol Chem
PubMed ID11744697
Herpesviral DNA packaging is a complex process resulting in unit-length genomes packed into preformed procapsids. This process is believed to be mediated by two packaging proteins, the terminase subunits. In the case of double-stranded DNA bacteriophages, the translocation of DNA was shown to be an energy-dependent process associated with an ... More
Urea-selective Concentrating Defect in Transgenic Mice Lacking Urea Transporter UT-B.
Authors Yang Baoxue; Bankir Lise; Gillespie Annemarie; Epstein Charles J; Verkman A S;
JournalJ Biol Chem
PubMed ID11792714
Urea transporter UT-B has been proposed to be the major urea transporter in erythrocytes and kidney-descending vasa recta. The mouse UT-B cDNA was isolated and encodes a 384-amino acid urea-transporting glycoprotein expressed in kidney, spleen, brain, ureter, and urinary bladder. The mouse UT-B gene was analyzed, and UT-B knockout mice ... More