Semaphorin III can function as a selective chemorepellent to pattern sensory projections in the spinal cord.
AuthorsMessersmith EK, Leonardo ED, Shatz CJ, Tessier-Lavigne M, Goodman CS, Kolodkin AL
JournalNeuron
PubMed ID7748562
'Distinct classes of primary sensory neurons in dorsal root ganglia subserve different sensory modalities, terminate in different dorsoventral locations in the spinal cord, and display different neurotrophin response profiles. Large diameter muscle afferents that terminate in the ventral spinal cord are NT-3 responsive, whereas small diameter afferents subserving pain and ... More
A nod factor binding lectin with apyrase activity from legume roots.
AuthorsEtzler ME, Kalsi G, Ewing NN, Roberts NJ, Day RB, Murphy JB
JournalProc Natl Acad Sci U S A
PubMed ID10318974
'A lectin isolated from the roots of the legume, Dolichos biflorus, binds to Nod factors produced by rhizobial strains that nodulate this plant and has a deduced amino acid sequence with no significant homology to any lectin reported to date. This lectin also is an enzyme that catalyzes the hydrolysis ... More
timrit Lengthens circadian period in a temperature-dependent manner through suppression of PERIOD protein cycling and nuclear localization.
AuthorsMatsumoto A, Tomioka K, Chiba Y, Tanimura T
JournalMol Cell Biol
PubMed ID10330175
A fundamental feature of circadian clocks is temperature compensation of period. The free-running period of ritsu (timrit) (a novel allele of timeless [tim]) mutants is drastically lengthened in a temperature- dependent manner. PER and TIM protein levels become lower in timrit mutants as temperature becomes higher. This mutation reduces per ... More
Kainate receptor subunits expressed in single cultured hippocampal neurons: molecular and functional variants by RNA editing.
To determine the kainate receptor subunits that are found in native kainate receptors, we have applied a multiplex PCR of cDNAs reverse transcribed from mRNA harvested from single cultured hippocampal neurons after electrophysiological recording. We found that all the cells showing rapidly desensitizing currents in response to kainate express the ... More
Genetic heterogeneity of hypervariable region 1 of the hepatitis C virus (HCV) genome and sensitivity of HCV to alpha interferon therapy
AuthorsSandres K, Dubois M, Pasquier C, Payen JL, Alric L, Duffaut M, Vinel JP, Pascal JP, Puel J, Izopet J
JournalJ Virol
PubMed ID10623727
Hepatitis C virus (HCV) populations persist in vivo as a mixture of heterogeneous viruses called quasispecies. The relationship between the genetic heterogeneity of these variants and their responses to antiviral treatment remains to be elucidated. We have studied 26 virus strains to determine the influence of hypervariable region 1 (HVR-1) ... More
Determinants of syncytium formation in microglia by human immunodeficiency virus type 1: role of the V1/V2 domains.
AuthorsShieh JT, Martin J, Baltuch G, Malim MH, Gonzalez-Scarano F.
JournalJ Virol
PubMed ID10623731
Microglia are the main reservoir for human immunodeficiency virus type 1 (HIV-1) in the central nervous system (CNS), and multinucleated giant cells, the result of fusion of HIV-1-infected microglia and brain macrophages, are the neuropathologic hallmark of HIV dementia. One potential explanation for the formation of syncytia is viral adaptation ... More
CpABC, a Cryptosporidium parvum ATP-binding cassette protein at the host-parasite boundary in intracellular stages.
AuthorsPerkins ME, Riojas YA, Wu TW, Le Blancq SM
JournalProc Natl Acad Sci U S A
PubMed ID10318953
The intracellular parasite Cryptosporidium parvum develops inside a vacuole at the apex of its epithelial host cell. The developing parasite is separated from the host cell cytoplasm by a zone of attachment that consists of an extensively folded membranous structure known as the feeder organelle. It has been proposed that ... More
Plant ribosome recycling factor homologue is a chloroplastic protein and is bactericidal in escherichia coli carrying temperature-sensitive ribosome recycling factor.
AuthorsRolland N, Janosi L, Block MA, Shuda M, Teyssier E, Miege C, Cheniclet C, Carde JP, Kaji A, Joyard J
JournalProc Natl Acad Sci U S A
PubMed ID10318906
We have isolated a protein, mature RRFHCP, from chloroplasts of spinach (Spinacia oleracea L.) that shows 46% sequence identity and 66% sequence homology with ribosome recycling factor (RRF) of Escherichia coli. RRF recycles ribosomes through disassembly of the posttermination complex. From the cDNA analysis and from the amino-terminal sequencing of ... More
A dominant T cell receptor beta-chain in response to a short ragweed allergen, Amb a 5.
AuthorsHuang SK, Yi M, Palmer E, Marsh DG
JournalJ Immunol
PubMed ID7751655
HLA-DR alpha, beta 1 *1501 (and the closely related DR alpha, beta 1 *1502) heterodimers are the primary class II MHC restriction elements controlling T cell responses to a ragweed pollen allergen, Amb a 5 (M(r) = 5000). Using a novel, quantitative, competitive PCR (QC-PCR) assay and a TCR beta-gene ... More
PCR-mediated recombination: a general method applied to construct chimeric infectious molecular clones of plasma-derived HIV-1 RNA.
AuthorsFang G, Weiser B, Visosky A, Moran T, Burger H
JournalNature Medicine
PubMed ID9930876
A PCR-based approach was developed that provides a powerful tool for engineering recombinant molecules without reliance on restriction sites. DNA sequences were first amplified by high-fidelity PCR using Pfu polymerase; they were then used both as 'megaprimers' and templates in subsequent asymmetric long PCR amplifications to form chimeric clones. To ... More