The refolding of different alpha-fetoprotein variants.
AuthorsLeong SS, Middelberg AP,
JournalProtein Sci
PubMed ID16882993
The effect of glycosylation on AFP foldability was investigated by parallel quantitative and qualitative analyses of the refolding of glycosylated and nonglycosylated AFP variants. Both variants were successfully refolded by dialysis from the denatured-reduced state, attaining comparable ... More
Identification and cardiotropic actions of sulfakinin peptides in the American lobster Homarus americanus.
AuthorsDickinson PS, Stevens JS, Rus S, Brennan HR, Goiney CC, Smith CM, Li L, Towle DW, Christie AE,
JournalJ Exp Biol
PubMed ID17575033
In arthropods, a group of peptides possessing a -Y((SO3H))GHM/LRFamide carboxy-terminal motif have been collectively termed the sulfakinins. Sulfakinin isoforms have been identified from numerous insect species. In contrast, members of this peptide family have thus far been isolated from just two crustaceans, the penaeid shrimp Penaeus monodon and Litopenaeus vannamei. ... More
Streptococcus iniae capsule impairs phagocytic clearance and contributes to virulence in fish.
Surface capsular polysaccharides play a critical role in protecting several pathogenic microbes against innate host defenses during infection. Little is known about virulence mechanisms of the fish pathogen Streptococcus iniae, though indirect evidence suggests that capsule could represent an important factor. The putative S. iniae capsule operon contains a homologue ... More
Sensitive multiplex real-time reverse transcription-PCR assay for the detection of human and animal noroviruses in clinical and environmental samples.
AuthorsWolf S, Williamson WM, Hewitt J, Rivera-Aban M, Lin S, Ball A, Scholes P, Greening GE,
JournalAppl Environ Microbiol
PubMed ID17616614
In this study, we developed a triplex real-time reverse transcription-PCR (RT-PCR)-based method that detects and distinguishes between noroviruses belonging to genogroups I, II, and III and that targets the junction between the regions of open reading frame 1 (ORF1) and ORF2. This is the first assay to include all three ... More