Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway(R) TOPO vector system.
AuthorsXu R, Li QQ,
JournalPlant Methods
PubMed ID18211693
ABSTRACT: BACKGROUND: In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway(R) cloning technology (Invitrogen Co.) has developed a fast and reliable alternative cloning methodology which uses ... More