Zero Background™ Cloning Kit - FAQs

查看更多产品信息 Zero Background™ Cloning Kit - FAQs (K250001)

2 个常见问题解答

What is the largest insert size tested with pZErO vectors?

BAC genomic clones as large as 25 kb have been reported to be cloned into the pZErO vectors.

Is the growth rate of TOP10 cells affected when harboring pZErO-1 plasmids?

Yes, the growth rate of TOP10 cells harboring pZErO-1 will be altered, and depends on how much functional ccdB protein is present. An insert fragment that does not completely disrupt the expression of the LacZ/ccdB fusion (usually very small inserts) will allow some production of the lethal protein which in turn will reduce the growth rate of the cell and produce lower plasmid yields. In contrast, a completely disrupted LacZ/ccdB fusion will allow normal (pUC level) growth and plasmid yield. Typical plasmid yields of pZErO-1 in LB/Zeocin media are 25% - 75% of pUC grown in LB/Amp. Colonies grown in SOB/Zeocin are more healthy and users can expect 75% - 200% of plasmid yield when compared to pUC grown in LB/Amp.