A Novel Homeobox Protein which Recognizes a TGT Core and Functionally Interferes with a Retinoid-responsive Motif
AuthorsBertolino, Reimund, Wildt-Perinic andClerc
JournalNature
PubMed ID1899916
'The synapsins are a family of closely related phosphoproteins (termed synapsins Ia, Ib, IIa and IIb) associated with synaptic vesicles and implicated in the short-term regulation of neurotransmitter release from nerve endings. During development, expression of the synapsins correlates temporally with synapse formation, but there has been no direct evidence ... More
Suppression of cell transformation by the cyclin-dependent kinase inhibitor p57KIP2 requires binding to proliferating cell nuclear antigen.
Authors Watanabe H; Pan Z Q; Schreiber-Agus N; DePinho R A; Hurwitz J; Xiong Y;
JournalProc Natl Acad Sci U S A
PubMed ID9465025
'Proper control of the mammalian cell cycle requires the function of cyclin-dependent kinase (CDK) inhibitors. The p21 family currently includes three distinct genes, p21, p27(Kip1), and p57(Kip2), that share a common N-terminal domain for binding to and inhibiting the kinase activity of CDK-cyclin complexes. The p21 protein also binds to ... More
Ligand binding to macrophage scavenger receptor-A induces urokinase- type plasminogen activator expression by a protein kinase-dependent signaling pathway.
AuthorsHsu HY, Hajjar DP, Khan KM, Falcone DJ
JournalJ Biol Chem
PubMed ID9422792
'Macrophage scavenger receptor-type A (MSR-A) has been implicated in the transmission of cell signals and the regulation of diverse cellular functions (Falcone, D. J., and Ferenc, M. J. (1988) J. Cell. Physiol. 135, 387-396; Falcone, D. J., McCaffrey, T. A., and Vergilio, J. A. (1991) J. Biol. Chem. 266, 22726-22732; ... More
A new cationic liposome reagent mediating nearly quantitative transfection of animal cells.
AuthorsRose JK, Buonocore L, Whitt MA
JournalBiotechniques
PubMed ID1867862
'One of the most efficient systems for the expression of genes in the cytoplasm of animal cells utilizes a recombinant vaccinia virus encoding the bacteriophage T7 RNA polymerase. Cells infected with this virus are transfected with plasmid DNAs containing the gene to be expressed under T7 promoter control. The major ... More
Interaction between growth arrest-DNA damage protein 34 and Src kinase Lyn negatively regulates genotoxic apoptosis.
Authors Grishin A V; Azhipa O; Semenov I; Corey S J;
JournalProc Natl Acad Sci U S A
PubMed ID11517336
'Genotoxic stresses activate intracellular signaling molecules, which lead to growth arrest, DNA repair, and/or apoptosis. Among these molecules are the growth arrest and DNA damage protein 34 (GADD34) and the Src-related protein tyrosine kinase Lyn. Here, we report that these two proteins physically and functionally interact to regulate DNA damage-induced ... More
Synthesis and anticonvulsant and neurotoxic properties of substituted N-phenyl derivatives of the phthalimide pharmacophore.
'This article describes a DNA-based vaccination strategy evaluated ex vivo with human cells. The vaccine was prepared by transferring tumor-derived genomic DNA to PCI-13 cells, a highly immunogenic tumor cell line (' ... More
Human fatty-acid synthase gene. Evidence for the presence of two promoters and their functional interaction.
AuthorsHsu MH, Chirala SS, Wakil SJ
JournalJ Biol Chem
PubMed ID8662758
'We have isolated and sequenced a genomic clone coding for the first three exons and the 5''-flanking region of the human fatty-acid synthase gene. The translation initiation site, ATG, is located in exon II. Primer extension and S1 nuclease analyses showed the presence of three transcription initiation (Ti) sites: Ti ... More
Sp1 is a component of the cytokine-inducible enhancer in the promoter of vascular cell adhesion molecule-1.
Authors Neish A S; Khachigian L M; Park A; Baichwal V R; Collins T;
JournalJ Biol Chem
PubMed ID7499419
'Transcription of the vascular cell adhesion molecule-1 (VCAM-1) gene in endothelial cells is induced by the inflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, and lipopolysaccharide. Previous studies demonstrated that the cytokine-response region in the VCAM1 promoter contains binding sites for the transcription factors nuclear factor-kappa B (NF-kappa B) and interferon ... More
p73beta Is Regulated by Protein Kinase Cdelta Catalytic Fragment Generated in the Apoptotic Response to DNA Damage.
'Protein kinase C (PKC) delta is cleaved by caspase-3 to a kinase-active catalytic fragment (PKCdeltaCF) in the apoptotic response of cells to DNA damage. Expression of PKCdeltaCF contributes to the induction of apoptosis by mechanisms that are presently unknown. Here we demonstrate that PKCdeltaCF associates with p73beta, a structural and ... More
Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling.
AuthorsUtani A, Momota Y, Endo H, Kasuya Y, Beck K, Suzuki N, Nomizu M, Shinkai H,
JournalJ Biol Chem
PubMed ID12826666
'The LG4 module of the laminin alpha 3 chain (alpha 3 LG4), a component of epithelial-specific laminin-5, has cell attachment activity and binds syndecan (Utani, A., Nomizu, M., Matsuura, H., Kato, K., Kobayashi, T., Takeda, U., Aota, S., Nielsen, P. K., and Shinkai, H. (2001) J. Biol. Chem. 276, 28779-28788). ... More
Modification of PDGFalpha receptor expression or function alters the metastatic phenotype of 3LL cells.
AuthorsFitzer-Attas CJ, Do MS, Feigelson S, Vadai E, Feldman M, Eisenbach L
JournalOncogene
PubMed ID9380406
'Functional PDGFalpha receptors are selectively expressed on highly lung-metastasizing clones of the 3LL Lewis lung carcinoma, but not on low-mestastatic clones. The highly metastatic clones are also growth induced in vitro by PDGF and lung conditioned medium. To investigate whether modification of PDGFalpha receptor expression or function can affect metastatic ... More
Electroporation for the efficient transfection of mammalian cells with DNA.
Authors Chu G; Hayakawa H; Berg P;
JournalNucleic Acids Res
PubMed ID3029703
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% ... More
The product of par-4, a gene induced during apoptosis, interacts selectively with the atypical isoforms of protein kinase C.
The atypical PKCs are involved in a number of important cellular functions, including cell proliferation. We report here that the product of the par-4 gene specifically interacts with the regulatory domains of zeta PKC and lambda/LPKC, which dramatically inhibits their enzymatic activity. This is particularly challenging, because expression of par-4 ... More
Novel Modulation of Neuronal Nicotinic Acetylcholine Receptors by Association with the Endogenous Prototoxin lynx1.
We previously identified lynx1 as a neuronal membrane molecule related to snake alpha-neurotoxins able to modulate nAChRs. Here, we show that lynx1 colocalizes with nAChRs on CNS neurons and physically associates with nAChRs. Single-channel recordings show that lynx1 promotes the largest of three current amplitudes elicited by ACh through alpha(4)beta(2) ... More
Modulation of TEL transcription activity by interaction with the ubiquitin-conjugating enzyme UBC9.
The E-26 transforming specific (ETS)-related gene TEL, also known as ETV6, is involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma. The encoded protein contains two functional domains: a helix-loop-helix (HLH) domain (also known as pointed domain) located at the N terminus and a DNA-binding ... More
Functional characterization of ETV6 and ETV6/CBFA2 in the regulation of the MCSFR proximal promoter.
AuthorsFears S, Gavin M, Zhang DE, Hetherington C, Ben-David Y, Rowley JD, Nucifora G
JournalProc Natl Acad Sci U S A
PubMed ID9050885
The ETV6/CBFA2 (TEL/AML1) fusion gene occurs as a result of the chromosome translocation t(12;21)(p13;q22) in up to 30% of children diagnosed with B cell precursor (cd10+, cd19+) acute lymphoblastic leukemia. Leukemic cells that have acquired the t(12;21) usually demonstrate loss of the remaining normal ETV6 (TEL) allele. Using reporter gene ... More
Myb and ets proteins are candidate regulators of c-kit expression in human hematopoietic cells.
AuthorsRatajczak MZ, Perrotti D, Melotti P, Powzaniuk M, Calabretta B, Onodera K, Kregenow DA, Machalinski B, Gewirtz AM
JournalBlood
PubMed ID9490676
Kit is a tyrosine kinase receptor that plays an important role in human hematopoietic cell growth. The promoter elements that modulate the gene's expression have not been extensively studied. Because of c-kit's acknowledged importance in hematopoiesis, we sought to address this issue in more detail. To perform these studies we ... More
Phosphorylation of CBP mediates transcriptional activation by neural activity and CaM kinase IV.
AuthorsImpey S, Fong AL, Wang Y, Cardinaux JR, Fass DM, Obrietan K, Wayman GA, Storm DR, Soderling TR, Goodman RH,
JournalNeuron
PubMed ID11970865
Activity-regulated transcription has been implicated in adaptive plasticity in the CNS. In many instances, this plasticity depends upon the transcription factor CREB. Precisely how neuronal activity regulates CREB remains unclear. To address this issue, we examined the phosphorylation state of components of the CREB transcriptional pathway. We show that NMDA ... More
Short technical reports. Effects of lipopolysaccharide on transfection efficiency in eukaryotic cells.
Authors Weber M; Möller K; Welzeck M; Schorr J;
JournalBiotechniques
PubMed ID8747659
The suitability of different purification methods for preparation of plasmid DNA for transfection into eukaryotic cells was systematically investigated. The reporter plasmid, pRSVcat, was prepared using several methods, and residual impurities in the preparations were quantitated. Transfection with these preparations was performed with several cell lines (HeLa, Huh7, COS7 and ... More
Ligand stimulation of a Ret chimeric receptor carrying the activating mutation responsible for the multiple endocrine neoplasia type 2B.
Authors Rizzo C; Califano D; Colucci-D'Amato G L; De Vita G; D'Alessio A; Dathan N A; Fusco A; Monaco C; Santelli G; Vecchio G; Santoro M; de Franciscis V;
JournalJ Biol Chem
PubMed ID8910618
Inherited activating mutations of Ret, a receptor tyrosine kinase, predispose to multiple endocrine neoplasia (MEN) types 2A and 2B and familial medullary thyroid carcinoma. To investigate the effects induced by acute stimulation of Ret, we transfected both PC12 and NIH 3T3 cells with a molecular construct in which the ligand-binding ... More
Differential nucleocytoplasmic trafficking between the related endocytic proteins Eps15 and Eps15R.
Authors Poupon Viviane; Polo Simona; Vecchi Manuela; Martin Gwendal; Dautry-Varsat Alice; Cerf-Bensussan Nadine; Di Fiore Pier Paolo; Benmerah Alexandre;
JournalJ Biol Chem
PubMed ID11777906
Eps15 and Eps15R are constitutive components of clathrin-coated pits that are required for clathrin-dependent endocytosis. The most striking difference between these two related proteins is that Eps15R is also found in the nucleus, whereas Eps15 is excluded from this compartment at steady state. To better understand the individual functions of ... More
Biochemical and biological characterization of a dodecameric CD4-Ig fusion protein: implications for therapeutic and vaccine strategies.
Authors Arthos James; Cicala Claudia; Steenbeke Tavis D; Chun Tae-Wook; Dela Cruz Charles; Hanback Douglas B; Khazanie Prateeti; Nam Daniel; Schuck Peter; Selig Sara M; Van Ryk Donald; Chaikin Margery A; Fauci Anthony S;
JournalJ Biol Chem
PubMed ID11805109
Drug toxicities associated with HAART lend urgency to the development of new anti-HIV therapies. Inhibition of viral replication at the entry stage of the viral life cycle is an attractive strategy because it prevents de novo infection. Soluble CD4 (sCD4), the first drug in this class, failed to suppress viral ... More
Structure of the m4 cholinergic muscarinic receptor gene and its promoter.
Authors Wood I C; Roopra A; Harrington C; Buckley N J;
JournalJ Biol Chem
PubMed ID8537349
Cholinergic muscarinic receptor genes are members of the G-protein receptor gene superfamily. In this study we describe the structure of the gene and promoter of the rat m4 muscarinic receptor gene. A rat cosmid clone containing the coding region for the m4 gene and 25 kilobases of upstream sequence was ... More
Lipopolysaccharide-mediated reactive oxygen species and signal transduction in the regulation of interleukin-1 gene expression.
Authors Hsu Hsien-Yeh; Wen Meng-Hsuan;
JournalJ Biol Chem
PubMed ID11940570
Lipopolysaccharide (LPS) stimulates macrophages to release inflammatory cytokines, interleukin-1 beta (IL-1), and tumor necrosis factor (TNF). LPS-induced TNF suppresses scavenger receptor functions in macrophages (van Lenten, B. J., and Fogelman, A. M. (1992) J. Immunol. 148, 112-116), which is regulated by TNF-mediated protein kinases (Hsu, H. Y., and Twu, Y. ... More
Mutational analysis of the insulin-like growth factor I prohormone processing site.
Authors Duguay S J; Lai-Zhang J; Steiner D F;
JournalJ Biol Chem
PubMed ID7615562
Insulin-like growth factor I (IGF-I) is a mitogenic peptide that is produced in most tissues and cell lines and plays an important role in embryonic development and postnatal growth. IGF-I is initially synthesized as a prohormone precursor that is converted to mature IGF-I by endoproteolytic removal of the carboxyl-terminal E-domain. ... More
The minor subunit splice variants, H2b and H2c, of the human asialoglycoprotein receptor are present with the major subunit H1 in different hetero-oligomeric receptor complexes.
Authors Yik Jasper H N; Saxena Amit; Weigel Paul H;
JournalJ Biol Chem
PubMed ID11943787
The hepatic asialoglycoprotein receptor (ASGP-R) is an endocytic receptor that mediates the internalization of desialylated glycoproteins and their delivery to lysosomes. The human ASGP-R is a hetero-oligomeric complex composed of H1 and H2 subunits. There are three naturally occurring H2 splice variants, designated H2a, H2b, and H2c, although the expression ... More
H2, the minor subunit of the human asialoglycoprotein receptor, trafficks intracellularly and forms homo-oligomers, but does not bind asialo-orosomucoid.
Authors Saxena Amit; Yik Jasper H N; Weigel Paul H;
JournalJ Biol Chem
PubMed ID12089159
The functional human hepatic asialoglycoprotein receptor (ASGP-R) is a hetero-oligomer composed of two subunits, designated H1 and H2, which are highly homologous. Despite their extensive homology, the major H1 subunit is stably expressed by itself, whereas in the absence of H1 most of the H2 subunits are degraded in the ... More
BRCA1-induced apoptosis involves inactivation of ERK1/2 activities.
AuthorsYan Y, Haas JP, Kim M, Sgagias MK, Cowan KH.
JournalJ Biol Chem
PubMed ID12082091
Mutation in the BRCA1 gene is associated with an increased risk of breast and ovarian cancer. Recent studies have shown that the BRCA1 gene product may be important in mediating responses to DNA damage and genomic instability. Previous studies have indicated that overexpression of BRCA1 can induce apoptosis or cell ... More