PureLink™ PCR Purification Kit, 50 preps - FAQs

View additional product information for PureLink™ PCR Purification Kit - FAQs (K310002, K310001)

9 product FAQs found

我能否使用PureLink PCR纯化试剂盒来纯化限制性内切酶酶切后的质粒DNA？

是的，您可以使用我们的试剂盒来纯化限制性内切酶酶切后的质粒DNA。

我能否使用PureLink PCR纯化试剂盒来开展凝胶纯化实验？

PureLink PCR纯化试剂盒与PureLink 凝胶纯化试剂盒两者间的缓冲液和操作步骤均不同。因此，您可以换用离心柱，但须注意使用正确的缓冲液和实验方案。

应用PureLink PCR纯化试剂盒（PureLink PCR Clean-Up Kit）可纯化的DNA片段大小范围是多少？

纯化的片段大小介于100 bp与12 kb之间。我们的试剂盒附带两款缓冲液，结合缓冲液（B2）与结合缓冲液HC（B3）。结合缓冲液HC（B3）能够去除引物二聚体和小于300bp的扩增失败的短PCR产物。

Can I use the PureLink PCR Purification Kit to clean up restriction digested plasmid DNA?

Yes, you can use our kit to clean up restriction digested plasmid DNA.

Can I use the PureLink PCR Purification Kit to do a gel purification?

The buffers and procedure are different for the PureLink PCR Purification Kit and PureLink Gel Purification Kit. Therefore, you can swap the column, but the right buffer and protocol must be used.

What are the minimum and maximum size ranges for DNA fragment cleanup using the PureLink PCR Clean-Up Kit?

The size range is between 100 bp and 12 kb. Our kit comes with two buffers, Binding Buffer (B2) and Binding Buffer HC (B3). The Binding Buffer HC (B3) eliminates primer-dimers and short failed PCR products that are smaller than 300 bp.

What is nested and semi-nested PCR?

Nested PCR requires two separate amplifications, with the first one using one set of PCR primers and the second one using internal, "nested" primers and 1% or less of the first PCR reaction as a template. Nested PCR is used when the target is present in low abundance or when non-specific PCR products are being produced along with the specific product. Semi-nested PCR is used when there is only enough sequence information to make a primer internal to one end of the primary PCR product such as in RACE (rapid amplification of cDNA ends).

Can I sequence PCR products rather than plasmids, and what is the advantage?

Yes, you can sequence PCR products. This is an excellent way to confirm the sequence of an insert when plasmid sequencing produces an unexpected sequence. The target DNA is amplified with a single set of primers and then sequenced using the same primers (although not all PCR primers work well as sequencing primers). Plasmid DNA can be PCR amplified using a one set of primers and the resulting product can then be purified using a column and then sequenced using the same primers. You can even get more specificity by using a sequencing primer that binds internally to one of the PCR primers.

How can I purify my PCR product?

Life Technologies offers a number of PCR clean-up kits for standard and 96-well plate PCR. Search for PCR Clean-Up at www.thermofisher.com.