RNAi techniques hold great promise for science,
from basic research all the way to human therapeutics,
and our understanding of the underlying
mechanisms have allowed us to design
better and more effective compounds. Fortunately,
much has also been learned about nonspecific
effects, such as off-target and stress
responses. These observations and discoveries
have led us to improved specificity ... More
Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis.
Although Rho regulates cytokinesis, little was known about the functions in mitosis of Cdc42 and Rac. We recently suggested that Cdc42 works in metaphase by regulating bi-orient attachment of spindle microtubules to kinetochores. We now confirm the role of Cdc42 by RNA interference and identify the mechanisms for activation and ... More
Stable plasma membrane levels of hCTR1 mediate cellular copper uptake.
AuthorsEisses JF, Chi Y, Kaplan JH,
JournalJ Biol Chem
PubMed ID15634665
The human copper transporter 1 (hCtr1), when heterologously overexpressed in insect cells, mediates saturable Cu uptake. In mammalian expression systems, a rapid Cu-dependent internalization of hCtr1 has been reported in cells that overexpress epitope-tagged hCtr1 when exposed to Cu in the external medium. This finding led to the suggestion that ... More