Proteomic identification of Bcl2-associated athanogene 2 as a novel MAPK-activated protein kinase 2 substrate.
AuthorsUeda K, Kosako H, Fukui Y, Hattori S,
JournalJ Biol Chem
PubMed ID15271996
'The p38 MAPK cascade is activated by various stresses or cytokines. Downstream of p38 MAPKs, there are diversification and extensive branching of signaling pathways. Fluorescent two-dimensional difference gel electrophoresis of phosphoprotein-enriched samples from HeLa cells in which p38 MAPK activity was either suppressed or activated enabled us to detect approximately ... More
Structural requirements for pre-microRNA binding and nuclear export by Exportin 5.
AuthorsZeng Y, Cullen BR,
JournalNucleic Acids Res
PubMed ID15356295
'The biogenesis and function of mature human microRNAs is dependent on the nuclear export of pre-microRNA precursors by Exportin 5 (Exp5). The precursor for the human miR-30 microRNA, which is a 63 nt long RNA hairpin bearing a 2 nt 3'' overhang, forms a specific complex with Exp5 and the ... More
Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis.
Although Rho regulates cytokinesis, little was known about the functions in mitosis of Cdc42 and Rac. We recently suggested that Cdc42 works in metaphase by regulating bi-orient attachment of spindle microtubules to kinetochores. We now confirm the role of Cdc42 by RNA interference and identify the mechanisms for activation and ... More
A potential role for RNA interference in controlling the activity of the human LINE-1 retrotransposon.
AuthorsSoifer HS, Zaragoza A, Peyvan M, Behlke MA, Rossi JJ,
JournalNucleic Acids Res
PubMed ID15701756
Long interspersed nuclear elements (LINE-1 or L1) comprise 17% of the human genome, although only 80-100 L1s are considered retrotransposition-competent (RC-L1). Despite their small number, RC-L1s are still potential hazards to genome integrity through insertional mutagenesis, unequal recombination and chromosome rearrangements. In this study, we provide several lines of evidence ... More
Stable plasma membrane levels of hCTR1 mediate cellular copper uptake.
AuthorsEisses JF, Chi Y, Kaplan JH,
JournalJ Biol Chem
PubMed ID15634665
The human copper transporter 1 (hCtr1), when heterologously overexpressed in insect cells, mediates saturable Cu uptake. In mammalian expression systems, a rapid Cu-dependent internalization of hCtr1 has been reported in cells that overexpress epitope-tagged hCtr1 when exposed to Cu in the external medium. This finding led to the suggestion that ... More