BLOCK-iT™ Complete Dicer RNAi Kit - FAQs

How are the BLOCK-iT Dicer RNAi Transfection and BLOCK-iT Complete Dicer RNAi Kit configured?

These kits are configured so that everything you need for your RNAi experiment is included. You just provide the dsRNA substrate or use the BLOCK-iTRNAi TOPO Transcription Kit (included in the BLOCK-iT Complete Dicer RNAi Kit) to generate your dsRNA. The BLOCK-iT Dicer RNAi Transfection kit provides the necessary reagents to generate enough diced product to do up to 150 transfection experiments in 24-well plate with up to five genes. This is significantly more transfection experiments than other Dicer kits.

The BLOCK-iT Dicer RNAi Transfection Kit includes:
(1) A high-quality preparation of BLOCK-iTDicer Enzyme RNAi
(2) Purification reagents to purify the diced siRNA (d-siRNA)
(3) Lipofectamine 2000 for high efficiency delivery of d-siRNA to mammalian cells

If you wish to use truly optimized Invitrogen reagents to prepare your dsRNA substrate and to complete the dicing reaction, we recommend the use of the BLOCK-iT Complete Dicer RNAi. An easy-to-use RNAi purification module with optimized protocols for isolating the dsRNA (BLOCK-iT RNAi TOPO Transcription Kit) or d-siRNA (BLOCK-iT Dicer RNAi Transfection Kit) is included with these kits. These are critical for best results. The purity of long dsRNA can affect how well this will act as a substrate for the dicing reaction. The optimized purification of the d-siRNA is essential, since if this is not purified completely away from the long dsRNA a general cell shutdown response can be triggered. Lipofectamine 2000 is also included in our BLOCK-iT Dicer RNAi Transfection Kit to assure high efficiency delivery and experimental success.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.