Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein.
AuthorsHsieh JC, Rattner A, Smallwood PM, Nathans J
JournalProc Natl Acad Sci U S A
PubMed ID10097073
'Biochemical studies of Wnt signaling have been hampered by difficulties in obtaining large quantities of soluble, biologically active Wnt proteins. In this paper, we report the production in Drosophila S2 cells of biologically active Xenopus Wnt8 (XWnt8). Epitope- or alkaline phosphatase-tagged XWnt8 proteins are secreted by concentrated S2 cells in ... More
Inhibitory Potency and Specificity of Subtilase-like Pro-protein Convertase (SPC) Prodomains.
'The SPCs (subtilisin-like pro-protein convertases) are a family of enzymes responsible for the proteolytic processing of numerous precursor proteins of the constitutive and regulated secretory pathways. SPCs are themselves synthesized as inactive zymogens. Activation of SPCs occurs via the intramolecular autocatalytic removal of the prodomain. SPC prodomains have been proposed ... More
A heterotrimeric death domain complex in Toll signaling.
Authors Sun Huaiyu; Bristow Benjamin N; Qu Guowei; Wasserman Steven A;
JournalProc Natl Acad Sci U S A
PubMed ID12351681
Signaling from the transmembrane receptor Toll to Rel-related transcription factors regulates dorsoventral patterning of the Drosophila embryo, as well as larval and adult immunity. To identify additional pathway components, we have used double-stranded RNA interference to investigate Drosophila counterparts of genes that regulate the mammalian Rel family member NF-kappaB. Experiments ... More