Overexpression, purification, and site-directed spin labeling of the Nramp metal transporter from Mycobacterium leprae.
Authors Reeve Ian; Hummell David; Nelson Nathan; Voss John;
JournalProc Natl Acad Sci U S A
PubMed ID12077319
'It has long been recognized that the pathogenicity of a broad range of intracellular parasites depends on the availability of transition metal ions, especially iron. Nramp1 (natural resistance-associated macrophage protein 1), a proton-coupled divalent metal ion transporter, has been identified as a controlling factor in the resistance or susceptibility to ... More
Ultrafast ligand rebinding in the heme domain of the oxygen sensors FixL and Dos: General regulatory implications for heme-based sensors.
Authors Liebl Ursula; Bouzhir-Sima Latifa; Negrerie Michel; Martin Jean-Louis; Vos Marten H.;
JournalProc Natl Acad Sci U S A
PubMed ID12271121
Heme-based oxygen sensors are part of ligand-specific two-component regulatory systems, which have both a relatively low oxygen affinity and a low oxygen-binding rate. To get insight into the dynamical aspects underlying these features and the ligand specificity of the signal transduction from the heme sensor domain, we used femtosecond spectroscopy ... More