Characterization of a Novel Drosophila melanogaster Galectin. EXPRESSION IN DEVELOPING IMMUNE, NEURAL, AND MUSCLE TISSUES.
Authors Pace Karen E; Lebestky Tim; Hummel Thomas; Arnoux Pascal; Kwan Kent; Baum Linda G;
JournalJ Biol Chem
PubMed ID11809773
'We have cloned and characterized the first galectin to be identified in Drosophila melanogaster. The amino acid sequence of Drosophila galectin showed striking sequence similarity to invertebrate and vertebrate galectins and contained amino acids that are crucial for binding beta-galactoside sugars. Confirming its identity as a galectin family member, the ... More
Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Transcriptional Activator Rta Is an Oligomeric DNA-Binding Protein That Interacts with Tandem Arrays of Phased A/T-Trinucleotide Motifs.
AuthorsLiao W, Tang Y, Kuo YL, Liu BY, Xu CJ, Giam CZ,
JournalJ Virol
PubMed ID12915555
'Kaposi''s sarcoma associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) encodes an immediate early transcriptional activator, Rta, which mediates viral reactivation from latency and lytic viral replication. Here we report the purification and characterizations of HHV-8 Rta and its interaction with Rta-responsive DNA elements. The Rta response element (RtaRE) in the promoter ... More
Assimilation of nicotinamide mononucleotide requires periplasmic AphA phosphatase in Salmonella enterica.
AuthorsGrose JH, Bergthorsson U, Xu Y, Sterneckert J, Khodaverdian B, Roth JR,
JournalJ Bacteriol
PubMed ID15968063
'Salmonella enterica can obtain pyridine from exogenous nicotinamide mononucleotide (NMN) by three routes. In route 1, nicotinamide is removed from NMN in the periplasm and enters the cell as the free base. In route 2, described here, phosphate is removed from NMN in the periplasm by acid phosphatase (AphA), and ... More
Transition state analogue inhibitors of purine nucleoside phosphorylase from Plasmodium falciparum.
Authors Kicska Gregory A; Tyler Peter C; Evans Gary B; Furneaux Richard H; Kim Kami; Schramm Vern L;
JournalJ Biol Chem
PubMed ID11707439
'Immucillins are logically designed transition-state analogue inhibitors of mammalian purine nucleoside phosphorylase (PNP) that induce purine-less death of Plasmodium falciparum in cultured erythrocytes (Kicska, G. A., Tyler, P. C., Evans, G. B., Furneaux, R. H., Schramm, V. L., and Kim, K. (2002) J. Biol. Chem. 277, 3226-3231). PNP is present ... More
Characterization of monomeric L1 metallo-beta -lactamase and the role of the N-terminal extension in negative cooperativity and antibiotic hydrolysis.
Authors Simm Alan M; Higgins Catherine S; Carenbauer Anne L; Crowder Michael W; Bateson John H; Bennett Peter M; Clarke Anthony R; Halford Stephen E; Walsh Timothy R;
JournalJ Biol Chem
PubMed ID11940588
The L1 metallo-beta-lactamase from Stenotrophomonas maltophilia is unique among this class of enzymes because it is tetrameric. Previous work predicted that the two regions of important intersubunit interaction were the residue Met-140 and the N-terminal extensions of each subunit. The N-terminal extension was also implicated in beta-lactam binding. Mutation of ... More