Cloning, expression, and catalytic mechanism of murine lysophospholipase I.
AuthorsWang A, Deems RA, Dennis EA
JournalJ Biol Chem
PubMed ID9139730
'A lysophospholipase (LysoPLA I) has been purified and characterized from the mouse macrophage-like P388D1 cell line (Zhang, Y. Y, and Dennis, E. A. (1988) J. Biol. Chem. 263, 9965-9972). This enzyme has now been sequenced, cloned, and expressed in Escherichia coli cells. The enzyme contains 230 amino acid residues with ... More
Phosphorylation of the periplasmic binding protein in two transport systems for arginine incorporation in Escherichia coli K-12 is unrelated to the function of the transport system.
AuthorsCelis RT, Leadlay PF, Roy I, Hansen A
JournalJ Bacteriol
PubMed ID9733684
In Escherichia coli K-12, the accumulation of arginine is mediated by two distinct periplasmic binding protein-dependent transport systems, one common to arginine and ornithine (AO system) and one for lysine, arginine, and ornithine (LAO system). Each of these systems includes a specific periplasmic binding protein, the AO-binding protein for the ... More