Simian-human immunodeficiency virus containing a human immunodeficiency virus type 1 subtype-E envelope gene: persistent infection, CD4(+) T-cell depletion, and mucosal membrane transmission in macaques.
AuthorsHimathongkham S, Halpin NS, Li J, Stout MW, Miller CJ, Luciw PA
JournalJ Virol
PubMed ID10933692
'The envelope (env) glycoprotein of human immunodeficiency virus type 1 (HIV-1) determines several viral properties (e.g., coreceptor usage, cell tropism, and cytopathicity) and is a major target of antiviral immune responses. Most investigations on env have been conducted on subtype-B viral strains, prevalent in North America and Europe. Our study ... More
Overexpression of Xenopus laevis growth hormone stimulates growth of tadpoles and frogs.
AuthorsHuang H, Brown DD
JournalProc Natl Acad Sci U S A
PubMed ID10618393
'The role of growth hormone (GH) in amphibian metamorphosis is ambiguous based on experiments in which mammalian GH was administered to tadpoles and frogs. We have reexamined the effects of GH by producing transgenic Xenopus laevis that overexpress the cDNA encoding X. laevis GH. These transgenic tadpoles take the same ... More
Identification of cardiac glycoside molecules as inhibitors of c-Myc IRES-mediated translation.
AuthorsDidiot MC, Hewett J, Varin T, Freuler F, Selinger D, Nick H, Reinhardt J, Buckler A, Myer V, Schuffenhauer A, Guy CT, Parker CN
JournalJ Biomol Screen
PubMed ID23150017
'Translation initiation is a fine-tuned process that plays a critical role in tumorigenesis. The use of small molecules that modulate mRNA translation provides tool compounds to explore the mechanism of translational initiation and to further validate protein synthesis as a potential pharmaceutical target for cancer therapeutics. This report describes the ... More
A dual-specificity aminoacyl-tRNA synthetase in the deep-rooted eukaryote giardia lamblia.
AuthorsBunjun S, Stathopoulos C, Graham D, Min B, Kitabatake M, Wang AL, Wang CC, Vivares CP, Weiss LM, Soll D
JournalProc Natl Acad Sci U S A
PubMed ID11078517
'Cysteinyl-tRNA (Cys-tRNA) is essential for protein synthesis. In most organisms the enzyme responsible for the formation of Cys-tRNA is cysteinyl-tRNA synthetase (CysRS). The only known exceptions are the euryarchaea Methanococcus jannaschii and Methanobacterium thermoautotrophicum, which do not encode a CysRS. Deviating from the accepted concept of one aminoacyl-tRNA synthetase per ... More
Cost-effective method to synthesize fluorescently labeled DNA size standards using cloned AFLP fragments.
AuthorsUeno S, Tsumura Y, Washitani I,
JournalBiotechniques
PubMed ID12813879
n/a
An isoform of ZBP-89 predisposes the colon to colitis.
AuthorsLaw DJ, Labut EM, Adams RD, Merchant JL,
JournalNucleic Acids Res
PubMed ID16517939
Alternative splicing enables expression of functionally diverse protein isoforms. The structural and functional complexity of zinc-finger transcription factor ZBP-89 suggests that it may be among the class of alternatively spliced genes. We identified a human ZBP-89 splice isoform (ZBP-89(DeltaN)), which lacks amino terminal residues 1-127 of the full-length protein (ZBP-89(FL)). ... More
Complex transcriptional control of the AZFa gene DDX3Y in human testis.
The human DEAD-box Y (DBY) RNA helicase (aka DDX3Y) gene is thought to be the major azoospermia factor a (AZFa) gene in proximal Yq11. Men with its deletion display no somatic pathologies, but suffer from complete absence of germ cells. Accordingly, DDX3Y protein is expressed only in the germline in ... More
Identification of a calcitonin-like diuretic hormone that functions as an intrinsic modulator of the American lobster, Homarus americanus, cardiac neuromuscular system.
AuthorsChristie AE, Stevens JS, Bowers MR, Chapline MC, Jensen DA, Schegg KM, Goldwaser J, Kwiatkowski MA, Pleasant TK, Shoenfeld L, Tempest LK, Williams CR, Wiwatpanit T, Smith CM, Beale KM, Towle DW, Schooley DA, Dickinson PS
JournalJ Exp Biol
PubMed ID20008368
In insects, a family of peptides with sequence homology to the vertebrate calcitonins has been implicated in the control of diuresis, a process that includes mixing of the hemolymph. Here, we show that a member of the insect calcitonin-like diuretic hormone (CLDH) family is present in the American lobster, Homarus ... More
Identification, tissue distribution and orexigenic activity of neuropeptide F (NPF) in penaeid shrimp.
AuthorsChristie AE, Chapline MC, Jackson JM, Dowda JK, Hartline N, Malecha SR, Lenz PH
JournalJ Exp Biol
PubMed ID21430216
The neuropeptide Fs (NPFs) are an invertebrate subgroup of the FMRFamide-like peptides, and are proposed by some to be the homologs of vertebrate neuropeptide Y. Although there is some information about the identity, tissue distribution and function of NPFs in insects, essentially nothing is known about them in crustaceans. We ... More