Multiple sites of V lambda diversification in cattle.
Authors:Lucier MR, Thompson RE, Waire J, Lin AW, Osborne BA, Goldsby RA
Journal:J Immunol
PubMed ID:9820519
'Ig repertoire diversification in cattle was studied in the ileal Peyer''s patch (IPP) follicles of young calvesand in the spleens of late first-trimester bovine fetuses. To investigate follicular diversification, individualIPP follicles were isolated by microdissection; VA diversity was examined by RT-PCR and subsequentcloning and sequencing. When 52 intrafollicular sequences from ... More