Positional cloning of the murine flavivirus resistance gene.
Authors Perelygin Andrey A; Scherbik Svetlana V; Zhulin Igor B; Stockman Bronislava M; Li Yan; Brinton Margo A;
JournalProc Natl Acad Sci U S A
PubMed ID12080145
'Inbred mouse strains exhibit significant differences in their susceptibility to viruses in the genus Flavivirus, which includes human pathogens such as yellow fever, Dengue, and West Nile virus. A single gene, designated Flv, confers this differential susceptibility and was mapped previously to a region of mouse chromosome 5. A positional ... More
Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota.
'Somatic hypermutation of immunoglobulin genes is a unique, targeted, adaptive process. While B cells are engaged in germinal centres in T-dependent responses, single base substitutions are introduced in the expressed Vh/Vl genes to allow the selection of mutants with a higher affinity for the immunizing antigen. Almost every possible DNA ... More
Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes.
Authors Vazquez G; Lievremont J P; St J Bird G; Putney J W Jr;
JournalProc Natl Acad Sci U S A
PubMed ID11553786
'Mammalian Trp proteins are candidates for plasma membrane calcium channels regulated by receptor activation or by intracellular calcium store depletion [capacitative calcium entry (CCE)]. One extensively investigated member of the Trp family, the human Trp3 (hTrp3), behaves as a receptor-activated, calcium-permeable, nonselective cation channel when expressed in cell lines and ... More
The major protein import receptor of plastids is essential for chloroplast biogenesis
AuthorsBauer J, Chen K, Hiltbunner A, Wehrli E, Eugster M, Schnell D, Kessler F
JournalNature
PubMed ID10646606
Light triggers the developmental programme in plants that leads to the production of photosynthetically active chloroplasts from non-photosynthetic proplastids. During this chloroplast biogenesis, the photosynthetic apparatus is rapidly assembled, mostly from nuclear-encoded imported proteins, which are synthesized in the cytosol as precursors with cleavable amino-terminal targeting sequences called transit sequences. ... More
Conserved bases in the TPsi C loop of tRNA are determinants for thermophile-specific 2-thiouridylation at position 54.
2-Thioribothymidine (s(2)T) is a post-transcriptionally modified nucleoside of U54 specifically found in thermophilic bacterial tRNAs. The 2-thiocarbonyl group of s(2)T54 is known to be responsible for the thermostability of tRNA. The s(2)T54 content in tRNA varies depending on the cultivation temperature, a feature that confers thermal adaptation of protein synthesis ... More