The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF.
Authors Laura Richard P; Witt Andrea S; Held Heike A; Gerstner Resi; Deshayes Kurt; Koehler Michael F T; Kosik Kenneth S; Sidhu Sachdev S; Lasky Laurence A;
JournalJ Biol Chem
PubMed ID11821434
'Erbin is a recently described member of the LAP (leucine-rich repeat and PDZ domain) protein family. We used a C-terminally displayed phage peptide library to identify optimal ligands for the Erbin PDZ domain. Phage-selected peptides were type 1 PDZ ligands that bound with high affinity and specificity to the Erbin ... More
Livin, a novel inhibitor of apoptosis protein family member.
Authors Kasof G M; Gomes B C;
JournalJ Biol Chem
PubMed ID11024045
'A novel human inhibitor of apoptosis protein (IAP) family member termed Livin was identified, containing a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain. The mRNA for livin was not detectable by Northern blot in most normal adult tissues with the exception of the placenta, but ... More
Btf, a novel death-promoting transcriptional repressor that interacts with Bcl-2-related proteins.
AuthorsKasof GM, Goyal L, White E
JournalMol Cell Biol
PubMed ID10330179
'The adenovirus E1B 19,000-molecular-weight (19K) protein is a potent inhibitor of apoptosis and cooperates with E1A to transform primary rodent cells. E1B 19K shows sequence and functional homology to the mammalian antiapoptotic gene product, Bcl-2. Like Bcl-2, the biochemical mechanism of E1B 19K function includes binding to and antagonization of ... More
The NHX family of Na+-H+ exchangers in Caenorhabditis elegans.
Authors Nehrke Keith; Melvin James E;
JournalJ Biol Chem
PubMed ID12021279
'Na+-H+ exchangers prevent cellular acidification by catalyzing the electroneutral exchange of extracellular sodium for an intracellular proton. To date, seven Na+-H+ exchangers have been identified in mammals, and although several members of this family have been extensively studied and characterized, it is clear that there are major gaps in our ... More
Functional domains and DNA-binding sequences of RFLAT-1/KLF13, a Krüppel-like transcription factor of activated T lymphocytes.
AuthorsSakamoto K, Yamaguchi S, Ando R, Miyawaki A, Kabasawa Y, Takagi M, Li CL, Perbal B, Katsube K.
JournalJ Biol Chem
PubMed ID12050170
'RFLAT-1/KLF13, a member of the Krüppel-like family of transcription factors, was identified as a transcription factor expressed 3-5 days after T lymphocyte activation. It binds to the promoter of the chemokine gene RANTES (regulated on activation normal T cell expressed and secreted) and regulates its ' ... More
Two defective heterozygous luteinizing hormone receptors can rescue hormone action.
Authors Lee ChangWoo; Ji Inhae; Ryu KiSung; Song YongSang; Conn P Michael; Ji Tae H;
JournalJ Biol Chem
PubMed ID11859079
'Luteinizing hormone receptor is a G protein-coupled receptor and consists of two halves: the N-terminal extracellular half (exodomain) and C-terminal membrane-associated half (endodomain). Hormone binds to the exodomain, and the resulting hormone-exodomain complex modulates the endodomain to generate signals. There are mutations that impair either hormone binding or signal generation. ... More
Authors Pinkoski Michael J; Droin Nathalie M; Green Douglas R;
JournalJ Biol Chem
PubMed ID12196549
'Members of the tumor necrosis factor (TNF) and TNF receptor families play important roles in inducing apoptosis and mediating the inflammatory response. Activated T lymphocytes can trigger the expression of Fas-ligand on non-lymphoid tissue, such as intestinal epithelial cells (IEC), and this, in turn, can induce apoptosis in the T ... More
Targeted disruption of Np95 gene renders murine embryonic stem cells hypersensitive to DNA damaging agents and DNA replication blocks.
NP95, which contains a ubiquitin-like domain, a cyclin A/E-Cdk2 phosphorylation site, a retinoblastoma (Rb) binding motif, and a ring finger domain, has been shown to be colocalized as foci with proliferating cell nuclear antigen in early and mid-S phase nuclei. We established Np95 nulligous embryonic stem cells by replacing the ... More
Endogenous 24(S),25-epoxycholesterol fine-tunes acute control of cellular cholesterol homeostasis.
AuthorsWong J, Quinn CM, Gelissen IC, Brown AJ,
JournalJ Biol Chem
PubMed ID17981807
Certain oxysterols, when added to cultured cells, are potent regulators of cholesterol homeostasis, decreasing cholesterol synthesis and uptake and increasing cholesterol efflux. However, very little is known about whether or not endogenous oxysterol(s) plays a significant role in cholesterol homeostasis. 24(S),25-Epoxycholesterol (24,25EC) is unique among oxysterols in that it is ... More
Cleavage of von Willebrand Factor Requires the Spacer Domain of the Metalloprotease ADAMTS13.
AuthorsZheng X, Nishio K, Majerus EM, Sadler JE,
JournalJ Biol Chem
PubMed ID12791682
ADAMTS13 consists of a reprolysin-type metalloprotease domain followed by a disintegrin domain, a thrombospondin type 1 motif (TSP1), Cys-rich and spacer domains, seven more TSP1 motifs, and two CUB domains. ADAMTS13 limits platelet accumulation in microvascular thrombi by cleaving the Tyr1605-Met1606 bond in von Willebrand factor, and ADAMTS13 deficiency causes ... More
Authors Wu Li; Bashirova Arman A; Martin Thomas D; Villamide Loreley; Mehlhop Erin; Chertov Andrei O; Unutmaz Derya; Pope Melissa; Carrington Mary; KewalRamani Vineet N;
JournalProc Natl Acad Sci U S A
PubMed ID11818554
Here, we describe the isolation and characterization of the rhesus macaque homolog for human DC-SIGN, a dendritic cell-specific C-type lectin. mac-DC-SIGN is 92% identical to hu-DC-SIGN. mac-DC-SIGN preserves the virus transmission function of hu-DC-SIGN, capturing and efficiently transducing simian and human immunodeficiency virus to target CD4(+) T cells. Surprisingly, however, ... More
The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor.
Authors Tang Tracy Tzu-Ling; Dowbenko Donald; Jackson Amanda; Toney Lisa; Lewin David A; Dent Alexander L; Lasky Laurence A;
JournalJ Biol Chem
PubMed ID11777915
The activation of the AKT/protein kinase B kinases by mutation of the PTEN lipid phosphatase results in enhanced survival of a diversity of tumors. This resistance to apoptosis is partly accomplished by the inhibition of genetic programs induced by a subfamily of forkhead transcription factors including AFX. Here we describe ... More
Activation of clg, a novel dbl family guanine nucleotide exchange factor gene, by proviral insertion at evi24, a common integration site in B cell and myeloid leukemias.
Authors Himmel Karen L; Bi Feng; Shen Haifa; Jenkins Nancy A; Copeland Neal G; Zheng Yi; Largaespada David A;
JournalJ Biol Chem
PubMed ID11839748
Retroviruses induce leukemia in inbred strains of mice by activating cellular proto-oncogenes and/or inactivating tumor suppressors. The proviral integration sites in these leukemias provide powerful genetic tags for disease gene identification. Here we show that Evi24, a common site of retroviral integration in AKXD B cell and BXH-2 myeloid leukemias, ... More
Molecular cloning and characterization of the human diacylglycerol kinase beta (DGKbeta) gene: alternative splicing generates DGKbeta isotypes with different properties.
Authors Caricasole Andrea; Bettini Ezio; Sala Cinzia; Roncarati Renza; Kobayashi Naoki; Caldara Fabrizio; Goto Kaoru; Terstappen Georg C;
JournalJ Biol Chem
PubMed ID11719522
Diacylglycerol kinases are key modulators of levels of diacylglycerol, a second messenger involved in a variety of cellular responses to extracellular stimuli. A number of diacylglycerol kinases encoded by separate genes are present in mammalian genomes. We have cloned cDNAs encoding several isoforms of the human homologue of the rat ... More
Overexpressed Human Mitochondrial Thioredoxin Confers Resistance to Oxidant-induced Apoptosis in Human Osteosarcoma Cells.
Authors Chen Yan; Cai Jiyang; Murphy T J; Jones Dean P;
JournalJ Biol Chem
PubMed ID12032145
Oxidative damage to mitochondria is a central mechanism of apoptosis induced by many toxic chemicals. Thioredoxin family proteins share a conserved Cys-X-X-Cys motif at their active center and play important roles in control of cellular redox state and protection against oxidative damage. In addition to the well studied cytosolic and ... More
Regulation of hyaluronidase activity by alternative mRNA splicing.
Authors Lokeshwar Vinata B; Schroeder Grethchen L; Carey Robert I; Soloway Mark S; Iida Naoko;
JournalJ Biol Chem
PubMed ID12084718
Hyaluronidase is a hyaluronic acid-degrading endoglycosidase that is present in many toxins and the levels of which are elevated in cancer. Increased concentration of HYAL1-type hyaluronidase correlates with tumor progression and is a marker for grade (G) 2 or 3 bladder cancer. Using bladder tissues and cells, prostate cancer cells, ... More
Cloning and Functional Expression of Human Short TRP7, a Candidate Protein for Store-operated Ca2+ Influx.
Authors Riccio Antonio; Mattei Cesar; Kelsell Rosemary E; Medhurst Andrew D; Calver Andrew R; Randall Andrew D; Davis John B; Benham Christopher D; Pangalos Menelas N;
JournalJ Biol Chem
PubMed ID11805119
The regulation and control of plasma membrane Ca(2+) fluxes is critical for the initiation and maintenance of a variety of signal transduction cascades. Recently, the study of transient receptor potential channels (TRPs) has suggested that these proteins have an important role to play in mediating capacitative calcium entry. In this ... More
Functional differences between TRPC4 splice variants.
Authors Schaefer Michael; Plant Tim D; Stresow Nicole; Albrecht Nadine; Schultz Gunter;
JournalJ Biol Chem
PubMed ID11713258
Functional characterizations of heterologously expressed TRPC4 have revealed diverse regulatory mechanisms and permeation properties. We aimed to clarify whether these differences result from different species and splice variants used for heterologous expression. Like the murine beta splice variant, rat and human TRPC4beta both formed receptor-regulated cation channels when expressed in ... More
Association of human aging with a functional variant of klotho.
Authors Arking Dan E; Krebsova Alice; Macek Milan Sr; Macek Milan Jr; Arking Albert; Mian I Saira; Fried Linda; Hamosh Ada; Dey Srabani; McIntosh Iain; Dietz Harry C;
JournalProc Natl Acad Sci U S A
PubMed ID11792841
Mice deficient in Klotho gene expression exhibit a syndrome resembling premature human aging. To determine whether variation in the human KLOTHO locus contributes to survival, we applied two newly characterized polymorphic microsatellite markers flanking the gene in a population-based association study. In a cohort chosen for its homogeneity, Bohemian Czechs, ... More