The pGlow TOPO® TA Expression Kit uses the Cycle 3 GFP mutant (1) as a reporter protein for transcriptional activity. Cycle 3 GFP is ideal for non-invasive analysis of promoter elements within intact, living cells. The fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods.
For Research Use Only. Not for use in diagnostic procedures.
规格
输送类型
转染
促进剂
无(无启动子)
产品类型
TOPO TA 表达试剂盒
报告基因
GFP (Cycle 3)
选择试剂(真核生物)
Geneticin™ (G-418)
蛋白标记
未标记
克隆方法
TOPO™-TA
载体
pGlow、TOPO-TA 载体
产品线
TOPO™
适用于(应用)
报告基因测定,克隆您自己的启动子
内容与储存
pGlow TOPO™ TA 表达试剂盒包含两盒。TOPO™ TA Cloning 盒包含线性化和拓扑异构酶 I-活化的 pGlow-TOPO™ 载体、dNTP、测序引物、盐溶液以及对照 PCR 模板和引物;在 -20°C 下储存。该 One Shot™ 盒含有 One Shot™ TOP10 大肠杆菌细胞、S.O.C.培养基和超螺旋对照质粒;在 -80°C 下储存。妥善储存时,可保证试剂盒稳定储存 6 个月。