RNAi techniques hold great promise for science,
from basic research all the way to human therapeutics,
and our understanding of the underlying
mechanisms have allowed us to design
better and more effective compounds. Fortunately,
much has also been learned about nonspecific
effects, such as off-target and stress
responses. These observations and discoveries
have led us to improved specificity ... More
Liver receptor homolog 1 is a negative regulator of the hepatic acute-phase response.
AuthorsVenteclef N, Smith JC, Goodwin B, Delerive P,
JournalMol Cell Biol
PubMed ID16943422
'The orphan nuclear receptor liver receptor homolog 1 (LRH-1) has been reported to play an important role in bile acid biosynthesis and reverse cholesterol transport. Here, we show that LRH-1 is a key player in the control of the hepatic acute-phase response. Ectopic expression of LRH-1 with adenovirus resulted in ... More
Trpm7 regulates cell adhesion by controlling the calcium dependent protease calpain.
AuthorsSu LT, Agapito MA, Li M, T N Simonson W, Huttenlocher A, Habas R, Yue L, Runnels LW,
JournalJ Biol Chem
PubMed ID16436382
'M-calpain is a protease implicated in the control of cell adhesion through focal adhesion disassembly. The mechanism by which the enzyme is spatially and temporally controlled is not well understood, particularly because calpain''s dependence on calcium exceeds the sub-micromolar concentrations normally observed in cells. Here we show that the channel-kinase ... More
Parathyroid hormone-related protein and ezrin are up-regulated in human lung cancer bone metastases.
AuthorsDeng X, Tannehill-Gregg SH, Nadella MV, He G, Levine A, Cao Y, Rosol TJ,
JournalClin Exp Metastasis
PubMed ID17370040
Lung cancer often metastasizes to bone in patients with advanced disease. Identification of the factors involved in the interactions between lung cancer cells and bone will improve the prevention and treatment of bone metastases. We identified changes in metastasis-related gene expression of human HARA lung squamous carcinoma cells co-cultured with ... More
Tumor inhibition by genomically integrated inducible RNAi-cassettes.
AuthorsKappel S, Matthess Y, Zimmer B, Kaufmann M, Strebhardt K,
JournalNucleic Acids Res
PubMed ID16945954
RNA interference (RNAi) has emerged as a powerful tool to induce loss-of-function phenotypes by post-transcriptional silencing of gene expression. In this study we wondered whether inducible RNAi-cassettes integrated into cellular DNA possess the power to trigger neoplastic growth. For this purpose inducible RNAi vectors containing tetracycline (Tet)-responsive derivatives of the ... More
The armadillo protein p0071 regulates Rho signalling during cytokinesis.
AuthorsWolf A, Keil R, Götzl O, Mun A, Schwarze K, Lederer M, Hüttelmaier S, Hatzfeld M
JournalNat Cell Biol
PubMed ID17115030
Cytokinesis requires the spatio-temporal coordination of cell-cycle control and cytoskeletal reorganization. Members of the Rho-family of GTPases are crucial regulators of this process and assembly of the contractile ring depends on local activation of Rho signalling. Here, we show that the armadillo protein p0071, unlike its relative p120(ctn), is localized ... More