Hsl7p, a negative regulator of Ste20p protein kinase in the Saccharomyces cerevisiae filamentous growth-signaling pathway
AuthorsFujita A, Tonouchi A, Hiroko T, Inose F, Nagashima T, Satoh R, Tanaka S
JournalProc Natl Acad Sci U S A
PubMed ID10411908
'In the budding yeast, Saccharomyces cerevisiae, protein kinases Ste20p (p21(Cdc42p/Rac)-activated kinase), Ste11p [mitogen-activated protein kinase (MAPK) kinase kinase], Ste7p (MAPK kinase), Fus3p, and Kss1p (MAPKs) are utilized for haploid mating, invasive growth, and diploid filamentous growth. Members of the highly conserved Ste20p/p65(PAK) protein kinase family regulate MAPK signal transduction pathways ... More
Essential myosin light chain as a target for caspase-3 in failing myocardium.
'Programmed cell death involves the activation of caspase proteases that can mediate the cleavage of vital cytoskeletal proteins. We have recently reported that, in failing cardiac myocytes, caspase-3 activation is associated with a reduction in contractile performance. In this study we used a modified yeast two-hybrid system to screen for ... More
In vivo analysis of the functional domains of the Drosophila splicing regulator RBP1.
AuthorsHeinrichs V, Baker BS
JournalProc Natl Acad Sci U S A
PubMed ID8990170
The Drosophila splicing factor RBP1 participates together with TRA and TRA-2 in the regulation of alternative splicing of doublesex (dsx) pre- mRNA. It does so by recognizing RBP1 RNA target sequences in the dsx pre-mRNA. RBP1 belongs to the Ser-Arg-rich (SR) protein family of splicing factors, which have in common ... More
Identification of promoter elements in mycobacteria: mutational analysis of a highly symmetricdual promoter directing the expression of replication genes of the Mycobacterium plasmidpAL5000.
AuthorsStolt P, Zhang Q, Ehlers S
JournalNucleic Acids Res
PubMed ID9862957
The 120 bp origin of replication (ori) for the Mycobacterium plasmid pAL5000 has been shown to comprise the binding sites for the replication protein RepB as well as the start site of transcription for the repA and repB genes, encoding the replication proteins RepA and RepB. In this work it ... More
Ubc9 is a novel modulator of the induction properties of glucocorticoid receptors.
Authors Kaul Sunil; Blackford John A Jr; Cho Sehyung; Simons S Stoney Jr;
JournalJ Biol Chem
PubMed ID11812797
The EC(50) of agonists and the partial agonist activity of antagonists are crucial parameters for steroid hormone control of gene expression and endocrine therapies. These parameters have been shown to be modulated by a naturally occurring cis-acting element, called the glucocorticoid modulatory element (GME) that binds two proteins, GMEB-1 and ... More