含 pCoBlast 的 DES™ 诱导试剂盒
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Invitrogen™

含 pCoBlast 的 DES™ 诱导试剂盒

DES™ 诱导试剂盒提供表达载体 pMT/V5-His 以及各种杀稻瘟菌素 (pCoBlast) 或潮霉素 (pCoHygro) 选择。该载体使用果蝇金属硫蛋白基因启动子，该启动子当将硫酸铜或氯化镉添加至培养基中时在 S2了解更多信息
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货号数量
K5120011 kit
货号 K512001
价格（CNY)
32,099.00
Each
添加至购物车
数量:
1 kit
价格（CNY)
32,099.00
Each
添加至购物车
DES™ 诱导试剂盒提供表达载体 pMT/V5-His 以及各种杀稻瘟菌素 (pCoBlast) 或潮霉素 (pCoHygro) 选择。该载体使用果蝇金属硫蛋白基因启动子，该启动子当将硫酸铜或氯化镉添加至培养基中时在 S2 细胞中诱导。pMT/V5-His 载体具有以下特点：

•小尺寸 (3.5 kb)，可改善 DNA 得率并提高亚克隆效率
•C 端 V5 抗原决定簇标签，可采用 Invitrogen 抗 V5 抗体进行快速检测
• C 端 6xHis 标签，可利用镍螯合树脂简单纯化重组融合蛋白

为了便于克隆，提供一组三个载体（A、B 和 C）。每个载体在不同读码框中具有相对于 C 端标签的编码序列的多个克隆位点。
仅供科研使用。不可用于诊断程序。
规格
产品类型诱导试剂盒
蛋白标记位置（至您的基因）C-端
数量1 kit
载体pMT
克隆方法Gateway™
产品线DES
促进剂MT
蛋白标记His 标签 (6x)、V5 抗原决定簇标签, V5 Epitope Tag
Unit SizeEach
内容与储存
各 DES™ 诱导型试剂盒含有 20 µg 各选择载体（pCoHygro 或 pCoBlast）、pMT/V5-His A、B、C 和 pMT/V5-His/lacZ、磷酸钙转染试剂盒、正向和反向引物（各 2 µg）、冷冻 S2 细胞、GIBCO™ Schneider 果蝇培养基以及适当的选择剂。S2 细胞在液氮中储存。培养基和选择剂在 +4°C 下储存。所有其他试剂在 -20°C 下储存。妥善储存时，所有组分可保证稳定储存 6 个月。

常见问题解答 (FAQ)

What is the doubling time of Drosophila S2 cells?

When grown at the optimal temperature of 27-28ºC, Drosophila S2 cells grow very quickly, with an approximate 14-16 hr doubling time. The cells can also be grown at 22-25ºC with doubling time of 24hrs. Humidity is an issue, so a shallow pan of water can be left in the incubator, but the incubator does not need to be turned on.

Growth medium is also a factor in doubling time - the doubling times listed here are for Sf900 II medium.

The cells can reach maximum densities of 1 to 2.5 x 10E7 cells/ml in shake flask cultures.

引用和文献 (1)

引用和文献
Abstract
A novel allelic variant of the human TSG-6 gene encoding an amino acid difference in the CUB module. Chromosomal localization, frequency analysis, modeling, and expression.
Authors: Nentwich Hilke A; Mustafa Zehra; Rugg Marilyn S; Marsden Brian D; Cordell Martin R; Mahoney David J; Jenkins Suzanne C; Dowling Barbara; Fries Erik; Milner Caroline M; Loughlin John; Day Anthony J;
Journal:J Biol Chem
PubMed ID:11854277
Tumor necrosis factor-stimulated gene-6 (TSG-6) encodes a 35-kDa protein, which is comprised of contiguous Link and CUB modules. TSG-6 protein has been detected in the articular joints of osteoarthritis (OA) patients, with little or no constitutive expression in normal adult tissues. It interacts with components of cartilage matrix (e.g. hyaluronan ... More