MagicMedia™ E. coli Expression Medium, Powder, 1 L - FAQs

MagicMedia™ E. coli Expression Medium, Powder, 1 L - FAQs

查看更多产品信息 MagicMedia™ E. coli Expression Medium, Powder - FAQs (K6810, K6815)

6 个常见问题解答

MagicMedia培养基能否使用较低的孵育温度？

可以，使用双温度表达方案已经验证过在18°C和25°C表达。

使用Champion pET载体和BL21 AI菌株时，能否使用MagicMedia培养基进行表达？

很遗憾，MagicMedia培养基不能与阿拉伯糖诱导剂一起工作。MagicMedia培养基需要IPTG诱导系统和完整的Lac操纵子。

什么是MagicMedia大肠杆菌表达培养基？

MagicMedia培养基是一种大肠杆菌表达培养基。它可使蛋白得率提高3-10倍，可用于含有功能性lac操纵子的T7调控型大肠杆菌载体和BL21 (DE3) 菌株，易于操作，并且可省去OD监测/诱导步骤。

Can I use a lower incubation temperature for MagicMedia Medium?

Yes, expression at 18 degrees C and 25 degrees C has been validated using the Dual Temperature expression protocol.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?

Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is MagicMedia Medium?

MagicMedia Medium is an E. coli expression medium. It produces 3-10 times higher protein yield, is compatible with T7 regulated E. coli vectors that contain a functional lac operon and BL21 (DE3) strains, is simple to use, and eliminates OD monitoring/induction steps.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.