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引用和文献 (26)
Invitrogen™
Bac-N-Blue™ 转染试剂盒
Bac-N-Blue™ 线性 DNA 专为与 pBlueBac 载体和 pMelBac 重组而设计。重组病毒具有全长、功能性了解更多信息
| 货号 | 数量 |
|---|---|
| K85501 又称 K855-01 | 5 transfections |
货号 K85501
又称 K855-01
价格（CNY)
13,847.00
Each
数量:
5 transfections
价格（CNY)
13,847.00
Each
Bac-N-Blue™ 线性 DNA 专为与 pBlueBac 载体和 pMelBac 重组而设计。重组病毒具有全长、功能性 lacZ
基因，可产生蓝色斑块。这便于鉴别和纯化。Bac-N-Blue™ DNA 可与基于多角体蛋白启动子的任何杆状病毒转移载体配合使用。Bac-N-Blue™ DNA
在三个位点进行线性化，其中一个位于对病毒增殖至关重要的基因中 (1)。这导致非重组病毒的减少，使得重组病毒的选择和纯化变得简单。除病毒 DNA 外，Bac-N-Blue™ 转染试剂盒还含有 Cellfectin™ 试剂，可实现高转染效率。
基因，可产生蓝色斑块。这便于鉴别和纯化。Bac-N-Blue™ DNA 可与基于多角体蛋白启动子的任何杆状病毒转移载体配合使用。Bac-N-Blue™ DNA
在三个位点进行线性化，其中一个位于对病毒增殖至关重要的基因中 (1)。这导致非重组病毒的减少，使得重组病毒的选择和纯化变得简单。除病毒 DNA 外，Bac-N-Blue™ 转染试剂盒还含有 Cellfectin™ 试剂，可实现高转染效率。
仅供科研使用。不可用于诊断程序。
规格
产品类型昆虫细胞转染试剂盒
数量5 transfections
载体pBlueBac
克隆方法限制性内切酶/MCS
产品线Bac-N-Blue
促进剂多角体蛋白
蛋白标记未标记
Unit SizeEach
内容与储存
Bac-N-Blue™ 转染试剂盒含有 5 x 0.5 µg 样品瓶装 Bac-NBlue™ 线性病毒 DNA 和 125 µL Cellfectin™ 试剂。
在 +4°C 下储存。妥善储存时，所有组分可保证稳定储存 6 个月。
在 +4°C 下储存。妥善储存时，所有组分可保证稳定储存 6 个月。
常见问题解答 (FAQ)
白色菌落不能在液体培养基中生长。我该怎么办？
为什么会出现蓝色菌落？为什么会出现中心为蓝色而边缘为白色的菌落？
我得到的主要是白色/野生型空斑，而不是蓝色/重组型空斑。哪里出错了？
感染细胞后，我在一个细胞中看到较大的多角体，而在相邻细胞中看到较小的多角体（数量更多）。这正常吗？
我担心得不到空斑。出现空斑需要几天？空斑通常为多大？
引用和文献 (26)
引用和文献
Abstract
OX40 stimulation by gp34/OX40 ligand enhances productive human immunodeficiency virus type 1 infection.
Journal:J Virol
PubMed ID:11435553
'OX40 is a member of the tumor necrosis factor (TNF) receptor superfamily and known to be an important costimulatory molecule expressed on activated T cells. To investigate the role of costimulation of OX40 in human immunodeficiency virus type 1 (HIV-1) infection by its natural ligand, gp34, the OX40-transfected ACH-2 cell
Identification of the major synaptojanin-binding proteins in brain.
Journal:J Biol Chem
PubMed ID:9079704
'Synaptojanin is a nerve-terminal enriched inositol 5-phosphatase thought to function in synaptic vesicle endocytosis, in part through interactions with the Src homology 3 domain of amphiphysin. We have used synaptojanin purified from Sf9 cells after baculovirus mediated expression in overlay assays to identify two major synaptojanin-binding proteins in rat brain.
Identification of a novel human eicosanoid receptor coupled to G(i/o).
Journal:J Biol Chem
PubMed ID:12065583
'We have conducted an in silico data base search for and cloned a novel G-protein-coupled receptor (GPCR) named TG1019. Dot and Northern blotting analyses showed that transcripts of the novel GPCR were expressed in various tissues except brain, and the expression was more intense in liver, kidney, peripheral leukocyte, lung,
VRILLE feeds back to control circadian transcription of Clock in the Drosophila circadian oscillator.
Journal:Neuron
PubMed ID:12546820
'The Drosophila circadian oscillator consists of interlocked period (per)/timeless (tim) and Clock (Clk) transcriptional/translational feedback loops. Within these feedback loops, CLK and CYCLE (CYC) activate per and tim transcription at the same time as they repress Clk transcription, thus controlling the opposite cycling phases of these transcripts. CLK-CYC directly bind
Accurate processing and secretion in the baculovirus expression system of an erythroid-cell-stimulating factor consisting of a chimaera of insulin-like growth factor II and an insect insulin-like peptide.
Journal:Biochem J
PubMed ID:8166627
'A synthetic gene encoding the signal peptide and the N-terminal sequence of bombyxin, an insect insulin-like peptide, and the 58 amino acids of the C-terminal sequence of human insulin-like growth factor II (IGF II) has been expressed using the baculovirus system. This synthetic chimaera was obtained by amplification of four