DreamTaq 热启动 DNA 聚合酶是一种增强型热启动 Taq DNA 聚合酶,比传统热启动 Taq DNA 聚合酶具有更高的 PCR 特异性、灵敏度和产量。DreamTaq 热启动 DNA 聚合酶在环境温度下对 DNA 聚合酶活性具有基于抗体的抑制作用,从而防止在扩增步骤之前扩增非特异性产物。配备 DreamTaq 热启动 DNA 聚合酶,则可采用与传统 Taq DNA 聚合酶相同的方案和循环条件,在室温下设置反应。
For Research Use Only. Not for use in diagnostic procedures.
规格
颜色Green
热启动内置热启动
反应次数200 次反应
突出端3'-A
聚合酶DreamTaq Green DNA 聚合酶
数量200 次反应
反应形式SuperMix 或预混液
运输条件干冰
最大浓度2X
适用于(应用)Hot-start PCR
高 GC PCR 扩增效果低
反应速度标准
Unit SizeEach
内容与储存
包含: • 4 x 1.25 mL DreamTaq 热启动 Green PCR 预混液 (2X) • 4 x 1.25 mL 无核酸酶水
于 -5 至 -30°C 储存。
常见问题解答 (FAQ)
Which nucleotide analogues can be used with DreamTaq Hot Start DNA Polymerase?
DreamTaq Hot Start DNA Polymerase can incorporate dUTP and a variety of modified nucleotides, such as: dITP, 7-deaza-dGTP, fluorescein-12-dUTP, Biotin-11-dUTP, dm5CTP, alpha-thio-dCTP, and aminoallyl-dUTP.
Are DreamTaq buffers the same for DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase?
Yes. 10X DreamTaq Buffer and 10X DreamTaq Green Buffer are the same for both polymerases.
My experiments require extra DreamTaq Buffer. Do you offer the buffer as a separate product?
Yes, DreamTaq Buffer (Cat. No. B65, 4 x 1.25 mL) and DreamTaq Green Buffer (Cat. No. B71, 4 x 1.25 mL) are available as stand-alone products.
Can DreamTaq Hot Start DNA Polymerase be used for amplification of bisulfite converted DNA?
Yes. DreamTaq Hot Start DNA Polymerase can read uracil in the template strand and therefore can be used for amplification of bisulfite converted DNA.
What is the limit of amplicon size using DreamTaq Hot Start DNA Polymerase?
DreamTaq Hot Start DNA Polymerase amplifies up to 6 kb from human genomic DNA and up to 20 kb from lambda DNA with high yields and specificity. Amplification of even longer fragments up to 9 kb from human genomic DNA has been demonstrated, but may require additional optimization of reaction conditions and primer design.