A composite Ets/Pit-1 binding site in the prolactin gene can mediate transcriptional responses to multiple signal transduction pathways.
AuthorsHoward PW, Maurer RA
JournalJ Biol Chem
PubMed ID7673116
'Binding sites for the tissue-specific transcription factor, Pit-1, are required for basal and hormonally induced prolactin gene transcription. Although Pit-1 is phosphorylated in response to several signaling pathways, the mechanism by which Pit-1 contributes to hormonal induction of gene transcription has not been defined. Recent reports suggest that phosphorylation of ... More
Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5).
Authors Bhakta S; Bartes A; Bowman K G; Kao W M; Polsky I; Lee J K; Cook B N; Bruehl R E; Rosen S D; Bertozzi C R; Hemmerich S;
JournalJ Biol Chem
PubMed ID10956661
'Based on sequence homology with a previously cloned human GlcNAc 6-O-sulfotransferase, we have identified an open reading frame (ORF) encoding a novel member of the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family termed GST-5 on the human X chromosome (band Xp11). GST-5 has recently been characterized as a novel GalNAc 6-O-sulfotransferase termed chondroitin ... More
Probable Identification of a Membrane-Associated Repressor of Bacillus subtilis DNA Replication as the E2 Subunit of the Pyruvate Dehydrogenase Complex
AuthorsAndrew Stein and William Firshein*
JournalJ Bacteriol
PubMed ID10735853
Two Bacillus subtilis lysogenic libraries were probed by an antibody specific for apreviously described membrane-associated inhibitor of B. subtilis DNA replication(J. Laffan and W. Firshein, Proc. Natl. Acad. Sci. USA 85:7452-7456, 1988). Threeclones that reacted strongly with the antibody contained an entire open reading frame.Sequencing identified one of the clones ... More
Sox9 and p300 cooperatively regulate chromatin-mediated transcription.
AuthorsFurumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H,
JournalJ Biol Chem
PubMed ID16109717
Chromatin structure is a fundamental component of gene regulation, expression, and cellular differentiation. We have previously reported that the multifunctional coactivator p300 is a member of the Sox9-related transcriptional apparatus and activates Sox9 (Sry-type high-mobility-group box 9)-dependent transcription during chondrogenesis. However, the mechanism of synergy between Sox9 and p300 in ... More