Efficient and scalable method for scaling up cell free protein synthesis in batch mode.
AuthorsVoloshin AM, Swartz JR,
JournalBiotechnol Bioeng
PubMed ID15937883
A novel method for general cell free system scale-up in batch mode was applied to expression of E. coli chloramphenicol acetyl transferase (CAT) and a GMCSF-scFv fusion protein being developed as a B-cell lymphoma vaccine candidate (GLH). Performance of two different E. coli based cell-free systems was evaluated using the ... More
Serine peptidase HTRA3 is closely associated with human placental development and is elevated in pregnancy serum.
AuthorsNie G, Li Y, Hale K, Okada H, Manuelpillai U, Wallace EM, Salamonsen LA,
JournalBiol Reprod
PubMed ID16251496
HTRA3 is a newly identified serine peptidase of the mammalian HTRA (high-temperature requirement factor A) family, that is upregulated dramatically during mouse placental development. The current study determined whether HTRA3 was involved in human placentation. During the menstrual cycle, HTRA3 was expressed primarily in the endometrial glands, being significantly upregulated ... More