Efficient and scalable method for scaling up cell free protein synthesis in batch mode.
AuthorsVoloshin AM, Swartz JR,
JournalBiotechnol Bioeng
PubMed ID15937883
A novel method for general cell free system scale-up in batch mode was applied to expression of E. coli chloramphenicol acetyl transferase (CAT) and a GMCSF-scFv fusion protein being developed as a B-cell lymphoma vaccine candidate (GLH). Performance of two different E. coli based cell-free systems was evaluated using the ... More