Mucosal immunity of the adolescent female genital tract.
AuthorsShrier LA, Bowman FP, Lin M, Crowley-Nowick PA,
JournalJ Adolesc Health
PubMed ID12606111
'This study sought to characterize mucosal immunity of the adolescent genital tract during the cycle and determine if adolescents have more suppressed immunoglobulin levels in the follicular phase than adults. Daily from cycle day 9 until ovulation, then every other day until menses, cervical secretions for IgA, IgG, and cytokines ... More
The effect of a T cell-specific NF-kappa B inhibitor on in vitro cytokine production and collagen-induced arthritis.
AuthorsGerlag DM, Ransone L, Tak PP, Han Z, Palanki M, Barbosa MS, Boyle D, Manning AM, Firestein GS,
JournalJ Immunol
PubMed ID10903776
'NF-kappa B plays a key role in the production of cytokines in inflammatory diseases. The effects of a novel T cell-specific NF-kappa B inhibitor, SP100030, were evaluated in cultured Jurkat cells and in murine collagen-induced arthritis (CIA). Chemical libraries were screened for NF-kappa B-inhibitory activity. SP100030, a compound identified in ... More
Need for an external proficiency testing program for cytokines, chemokines, and plasma markers of immune activation.
AuthorsFahey JL, Aziz N, Spritzler J, Plaeger S, Nishanian P, Lathey JL, Seigel J, Landay AL, Kilarui R, Schmitz JL, White C, Wara DW, Akridge R, Cutili J, Douglas SD, Reuben J, Shearer WT, Nokta M, Polland R, Schooley R, Asthana D, Mizrachi Y, Waxdal M,
JournalClin Diagn Lab Immunol
PubMed ID10882648
'An external evaluation program for measuring the performance of laboratories testing for cytokines and immune activation markers in biological fluids was developed. Cytokines, chemokines, soluble cytokine receptors, and other soluble markers of immune activation (CSM) were measured in plasma from a healthy human immunodeficiency virus (HIV)-seronegative reference population and from ... More
Impaired antigen-presenting cell function contributes to T-cell hyporesponsiveness in stable lung transplant recipients.
AuthorsKnoop C, Ismaili J, Bulté F, Abramowicz D, Estenne M, Goldman M,
JournalTransplantation
PubMed ID10798749
'Peripheral blood mononuclear cells (PBMC) of stable renal or cardiac transplant recipients were previously shown to respond to allogeneic cells but not to soluble protein antigens. The aim of the present study was to assess the T-cell and antigen-presenting cell (APC) functions of stable lung transplant (LT) recipients. We obtained ... More
Inhibition of cytokine gene transcription by the human recombinant histamine-releasing factor in human T lymphocytes.
AuthorsVonakis BM, Sora R, Langdon JM, Casolaro V, MacDonald SM,
JournalJ Immunol
PubMed ID14500674
Human recombinant histamine-releasing factor (HrHRF) preincubation enhances the secretion of histamine, IL-4, and IL-13 from FcepsilonRI-stimulated human basophils. In GM-CSF-primed human eosinophils, HrHRF increases IL-8 production. Our recent experiments were designed to evaluate the effects of HrHRF on human T cell cytokine production. Purified T cells were preincubated with GST-tagged ... More
Activation of human CD4+ cells with CD3 and CD46 induces a T-regulatory cell 1 phenotype.
AuthorsKemper C, Chan AC, Green JM, Brett KA, Murphy KM, Atkinson JP,
JournalNature
PubMed ID12540904
The immune system must distinguish not only between self and non-self, but also between innocuous and pathological foreign antigens to prevent unnecessary or self-destructive immune responses. Unresponsiveness to harmless antigens is established through central and peripheral processes. Whereas clonal deletion and anergy are mechanisms of peripheral tolerance, active suppression by ... More
Anti-CD3 monoclonal antibody in new-onset type 1 diabetes mellitus.
AuthorsHerold KC, Hagopian W, Auger JA, Poumian-Ruiz E, Taylor L, Donaldson D, Gitelman SE, Harlan DM, Xu D, Zivin RA, Bluestone JA,
JournalN Engl J Med
PubMed ID12037148
Type 1 diabetes mellitus is a chronic autoimmune disease caused by the pathogenic action of T lymphocytes on insulin-producing beta cells. Previous clinical studies have shown that continuous immune suppression temporarily slows the loss of insulin production. Preclinical studies suggested that a monoclonal antibody against CD3 could reverse hyperglycemia at ... More
Modulation of T-cell function by (R)- and (S)-isomers of albuterol: anti-inflammatory influences of (R)-isomers are negated in the presence of the (S)-isomer.
AuthorsBaramki D, Koester J, Anderson AJ, Borish L,
JournalJ Allergy Clin Immunol
PubMed ID11897990
beta(2)-Adrenergic agonists interact with specific receptors on T lymphocytes to mediate anti-inflammatory activities. However, anti-inflammatory effects are not observed when beta(2)-adrenergic agonists are administered in vivo as racemates. We hypothesized that anti-inflammatory influences are mediated by the (R)-isomer and are masked in the additional presence of the (S)-isomer. Antigen-specific T-cell ... More
Activation of human T cells by FcR nonbinding anti-CD3 mAb, hOKT3gamma1(Ala-Ala).
AuthorsHerold KC, Burton JB, Francois F, Poumian-Ruiz E, Glandt M, Bluestone JA,
JournalJ Clin Invest
PubMed ID12569167
Dimeric Fc receptor (FcR) nonbinding anti-CD3 antibodies have been developed to minimize toxicities associated with classical anti-CD3 monoclonal antibodies (e.g., OKT3). Studies with murine analogs of non-FcR-binding antibodies have shown reduced mitogenicity compared to OKT3. In a trial of an FcR nonbinding humanized anti-CD3 mAb hOKT3gamma1(Ala-Ala) for treatment of patients ... More
Maternal Trypanosoma cruzi infection upregulates capacity of uninfected neonate cells To produce pro- and anti-inflammatory cytokines.
AuthorsVekemans J, Truyens C, Torrico F, Solano M, Torrico MC, Rodriguez P, Alonso-Vega C, Carlier Y,
JournalInfect Immun
PubMed ID10948177
The possibility of maternal in utero modulation of the innate and/or adaptive immune responses of uninfected newborns from Trypanosoma cruzi-infected mothers was investigated by studying the capacity of their whole blood cells to produce cytokines in response to T. cruzi lysate or lipopolysaccharide-plus-phytohemagglutinin (LPS-PHA) stimulation. Cells of such newborns occasionally ... More
Investigating the role of immunomodulation for colon cancer prevention: results of an in vivo dose escalation trial of levamisole with immunologic endpoints.
AuthorsHolcombe RF, Milovanovic T, Stewart RM, Brodhag TM,
JournalCancer Detect Prev
PubMed ID11341354
The potential role of immunomodulatory agents for colon cancer prevention has not been studied systematically. Levamisole (LMS), which is immunostimulatory, is synergistic with 5-fluorouracil in the adjuvant therapy of patients with stage III colon cancer. This pilot study was initiated to explore the potential utility of LMS as a colon ... More
Cytokine production and T-cell activation by macrophage-dendritic cells generated for therapeutic use.
AuthorsCoronel A, Boyer A, Franssen JD, Romet-Lemonne JL, Fridman WH, Teillaud JL,
JournalBr J Haematol
PubMed ID11552997
Clinical grade ex vivo-generated antigen-presenting cells, macrophage-dendritic cells (MAC-DCs) or macrophage-activated killers (MAKs) were derived from peripheral blood mononuclear cells (PBMCs). Cultures (7 d) were performed in non-adherent conditions in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and either interleukin 13 (IL-13) or dihydroxy-vitamin D3 respectively. MAKs were activated during ... More