'IL-15 is a novel cytokine active through the IL-2R/betagamma. Since several human melanoma cell lines display functional IL-2Rs, we studied the IL-15/melanoma cells interactions. Ten out of 17 melanoma cell lines express the IL-15 transcript and four of them express levels of IL-15 mRNA similar to those detected in control ... More
TNF stimulates nuclear export and secretion of IL-15 by acting on CRM1 and ARF6.
AuthorsOuyang S, Hsuchou H, Kastin AJ, Pan W,
Journal
PubMed ID23950892
'Interleukin (IL)-15 is a ubiquitously expressed cytokine that in the basal state is mainly localized intracellularly, including the nucleus. Unexpectedly, tumor necrosis factor-a (TNF) time-dependently induced nuclear export of IL-15Ra and IL15. This process was inhibited by leptomycine B (LMB), a specific inhibitor of nuclear export receptor chromosomal region maintenance ... More
Functional T-cell anergy in a case of persistent polyclonal B-cell lymphocytosis.
The T-cell population of a patient with persistent polyclonal B-cell lymphocytosis (PPBL) presenting with an intermittent Epstein-Barr virus (EBV)-associated disease was studied. Unstimulated T-cells did not express CD40 ligand (CD40L), whereas activation with IL-2 led to expression of this costimulatory molecule. CD40L expression was inhibited upon incubation with the supernatant ... More
Dermal fibroblasts sustain proliferation of activated T cells via membrane-bound interleukin-15 upon long-term stimulation with tumor necrosis factor-alpha.
AuthorsRappl G, Kapsokefalou A, Heuser C, Rössler M, Ugurel S, Tilgen W, Reinhold U, Abken H,
JournalJ Invest Dermatol
PubMed ID11168804
In chronic inflammatory conditions, mononuclear cells infiltrate the connective tissue attracted by fibroblast-secreted chemokines. The role of fibroblasts in sustaining the lymphocyte immune response upon cellular infiltration is so far unresolved. We here report that, upon prolonged stimulation with tumor necrosis factor-alpha, dermal fibroblasts enhance proliferation of activated T cells ... More
Human hepatocytes express an array of proinflammatory cytokines after agonist stimulation or bacterial invasion.
Inflammatory cells infiltrate the liver in response to microbial infection or hepatic injury. To assess the potential role hepatocytes may play in initiating or amplifying the acute inflammatory response in the liver, we used three human hepatocyte cell lines and primary human hepatocyte cultures to characterize the repertoire of cytokines ... More
Dengue virus infection of human endothelial cells leads to chemokine production, complement activation, and apoptosis.
AuthorsAvirutnan P, Malasit P, Seliger B, Bhakdi S, Husmann M,
JournalJ Immunol
PubMed ID9834124
Dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS) are severe complications of secondary dengue virus (DV) infection. Vascular leakage, hemorrhagic diathesis and complement activation are the hallmarks of the disease. The short-lived nature of the plasma leakage syndrome has led to the conclusion that altered permeability is most likely effected ... More