STAT5 plays a critical role in regulating the 5'-flanking region of the porcine whey acidic protein gene in transgenic mice.
AuthorsJi MR, Lee SI, Jang YJ, Jeon MH, Kim JS, Kim KW, Park JK, Yoo JG, Jeon IS, Kwon DJ, Park CK, Byun SJ,
Journal
PubMed ID26256125
'The mammary gland serves as a valuable bioreactor system for the production of recombinant proteins in lactating animals. Pharmaceutical-grade recombinant protein can be harvested from the milk of transgenic animals that carry a protein of interest under the control of promoter regions genes encoding milk proteins. Whey acidic protein (WAP), ... More
Defective synthesis of granulocyte-colony stimulating factor in pegylated interferon-alpha treated chronic hepatitis C patients with declining leukocyte counts.
AuthorsDurante-Mangoni E, Iardino P, Utili R, Adinolfi LE, Ruggiero G,
JournalAntivir Ther
PubMed ID16964833
'Pegylated-interferon-alpha (peg-IFN-alpha) is the mainstay of treatment for chronic hepatitis C (CHC). Treatment is often complicated by neutropaenia due to inhibition of haematopoiesis. However, there are no data on the kinetics of granulocyte-colony stimulating factor (G-CSF), a major neutrophil growth factor, in this setting. We therefore evaluated G-CSF synthesis in ... More
IL-17 and IL-17F modulate GM-CSF production by lung microvascular endothelial cells stimulated with IL-1beta and/or TNF-alpha.
AuthorsNumasaki M, Tomioka Y, Takahashi H, Sasaki H,
JournalImmunol Lett
PubMed ID15388258
'In this study, we investigated the roles of CD4 T cell cytokines IL-17 and IL-17F in GM-CSF production from lung microvascular endothelial cells (LMVECs). While a wide range of doses of IL-17 or IL-17F alone did not induce GM-CSF release from LMVECs, IL-17 had an enhancing effect on macrophage-derived IL-1beta- ... More
Anti-cytokine autoantibodies are ubiquitous in healthy individuals.
AuthorsWatanabe M, Uchida K, Nakagaki K, Kanazawa H, Trapnell BC, Hoshino Y, Kagamu H, Yoshizawa H, Keicho N, Goto H, Nakata K,
JournalFEBS Lett
PubMed ID17470370
Anti-cytokine autoantibodies in healthy individuals have been widely reported but the occurrence is variable and inconstant. We hypothesized that cytokine-binding in vivo may explain their variable and infrequent detection. Therefore, we focused on the detection of the cytokine-autoantibody complexes and found that anti-cytokine autoantibody to IL-2, IL-8, tumor necrosis factor-alpha, ... More
Effects of lipoxin A(4) on lipopolysaccharide induced proliferation and reactive oxygen species production in RAW264.7 macrophages through modulation of G-CSF secretion.
AuthorsZhou XY, Wu P, Zhang L, Xiong W, Li YS, Feng YM, Ye DY,
JournalInflamm Res
PubMed ID17687517
To investigate the effects of lipoxin A(4) (LXA(4)) on lipopolysaccharide (LPS) induced proliferation and reactive oxygen species production in RAW264.7 macrophages. RAW264.7 macrophages were treated with or without LPS in the absence or presence of LXA(4). In another experiment, the cells were incubated with rmG-CSF (recombinant mouse granulocyte-colony stimulating factor) ... More
Recruitment of immature neutrophils in peripheral blood following leukocytapheresis therapy for rheumatoid arthritis.
AuthorsOkawa-Takatsuji M, Nagatani K, Nakajima K, Itoh K, Kano T, Nagashio C, Takahashi Y, Aotsuka S, Mimori A,
JournalJ Clin Apher
PubMed ID18095302
The objective of this study is to evaluate the cellular mechanism underlying filtration leukocytapheresis (LCAP) therapy for the treatment of rheumatoid arthritis (RA). Thirteen patients with refractory RA each underwent three sessions of LCAP. Before (pre-) and after (post-) the completion of the first LCAP session, peripheral blood was sampled ... More