PicoPure™ RNA 分离试剂盒
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Applied Biosystems™

PicoPure™ RNA 分离试剂盒

Arcturus™ PicoPure™ RNA 分离试剂盒被设计用于从少于十个细胞甚至单个细胞中持续回收高质量的总 RNA。该试剂盒旨在提供一致、高效的 RNA 回收率而不损害质量。少量洗脱液可让您最大程度提高少量细胞的了解更多信息
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货号样品数量
KIT020440 samples
KIT0214200 samples
货号 KIT0204
价格(CNY)
4,952.00
Each
添加至购物车
样品数量:
40 samples
价格(CNY)
4,952.00
Each
添加至购物车
Arcturus™ PicoPure™ RNA 分离试剂盒被设计用于从少于十个细胞甚至单个细胞中持续回收高质量的总 RNA。该试剂盒旨在提供一致、高效的 RNA 回收率而不损害质量。少量洗脱液可让您最大程度提高少量细胞的 RNA 回收率,以便与基因表达分析配合使用。

关键产品特点:

•灵敏度更佳—甚至从单个细胞中分离 RNA
•效率更高—最大化样品的 RNA 回收率
•简化浓缩—小体积洗脱
•灵活应用—与多种试管尺寸兼容
•综合防止污染—柱盖确保样品保持无 RNase

甚至从单个细胞中分离 RNA
PicoPure™ RNA 分离试剂盒被设计用于从少于十个细胞中持续回收高质量总 RNA。使用该试剂盒达到的高回收率不仅对于包含皮克 RNA 的小样品非常有价值,也对于含有高达 100 µg RNA 的样品有价值。由于 RNA 在低至 10 µ l 缓冲液中洗脱,其可用于基因表达分析,而无需任何真空浓缩。此试剂盒有两种规格,足够用于40或200次分离。

获得优越的 RNA 回收率
通常无法替代较小的有价值的样品。对这些珍贵样品进行基因表达研究需要获得最大的 RNA 回收率。比较研究显示,使用小 RNA 含量较少的细胞或组织样品,PicoPure™ 试剂盒显著优于其他商业总 RNA 分离试剂盒的效率。

取消了耗时的 RNA 浓缩
简单的分离方案包含细胞 RNA 提取,然后将提取物上样到专门设计的 MiraCol™ 纯化柱上以结合 RNA。洗去杂质后,仅用 10 µL 缓冲液洗脱 RNA,即可直接使用,无需真空浓缩或转移到新反应管中。

高得率纯化柱
PicoPure™ RNA 分离试剂盒以独特的高回收率 MiraCol™ 纯化柱为特点,其设计用于从显微镜样品中极大程度提高回收率。这类柱具有方便的集成盖,有助于确保样品无 RNase。此外,该试剂盒还配备了所有必要缓冲液、易于遵循的用户指南、经优化的处理激光捕获显微切割 (LCM) 样品的方案和从较大样品中分离 RNA 的方案。

递送直接可用于扩增和微阵列分析的 RNA
使用 PicoPure ™ RNA 试剂盒分离的 RNA 即可使用,无需真空浓缩,用于使用 RiboAmp™ RNA 扩增试剂盒扩增。两个试剂盒一起实现了高度可重现的基因表达结果。

保留低丰度 mRNA
PicoPure™ RNA 分离试剂盒通过分离过程可保留所有丰度类别中的 mRNA,将目标基因的丢失降至最低。低、中和高丰度基因一致被回收并可用于表达分析。

维持高 RNA 质量
PicoPure™ RNA 分离过程可获得高质量 RNA。从 LCM 样品中制备的总细胞 RNA 在分离后仍保持完整,增加了对后续分子分析的置信度。
仅供科研使用。不可用于诊断程序。
规格
最终产品类型总 RNA、转录组 RNA、micro RNA
适用于(应用)微阵列分析,实时荧光定量 PCR(qPCR)
适用于(设备)ArcturusXT™ LCM 仪器
高通量能力不兼容高通量应用(手动)
样品数量40 samples
产品线PicoPure™
数量40 samples
原始材料量低至单细胞。
产品规格离心柱
分离技术离心柱
样品类型激光捕获显微切割 (LCM), 组织(冷冻)
目标总 RNA
Unit SizeEach
内容与储存
提取缓冲液、纯化试剂、纯化柱、微量离心管。室温保存

常见问题解答 (FAQ)

How many cells are needed for the PicoPure RNA Isolation Kit?

The PicoPure RNA Isolation Kit can be used with as little as a single cell to samples with up to 100 µg of RNA.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What are the RNA size limits for PicoPure RNA Isolation Kit?

The PicoPure RNA Isolation Kit is not recommended for isolation of RNA smaller than 100 nucleotides.

I am having trouble assessing the RNA quality from formalin-fixed, paraffin-embedded samples after laser capture microdissection (LCM). Can you offer some tips?

Formalin-fixed paraffin-embedded (FFPE) samples introduce unique challenges for gene expression profiling and gene expression quantitation, including chemical modification and fragmentation of RNA molecules. The initial paraffin blot preparation and storage significant affect the RNA quality. Archival FFPE samples present additional challenges due to increased RNA degradation over time. As a result, not all FFPE samples contain high quality RNA. 

In addition, a typical Bioanalyzer profile of RNA from an FFPE sample can look fragmented and degraded and as a result, that approach may not be useful in determining the transcribability of your RNA. Therefore, a tissue scrape test is recommended. You can use the entire adjacent section to isolate RNA and to access the RNA quantity and quality for intact RNA. Two sets of β-actin gene-specific primers designed to amplify short segments of DNA within the β-actin target sequence from the 5’ and 3’ of the β-actin gene can be used to estimate the amount of RNA in a given sample in relation to β-actin content using real-time PCR. The RNA quantity derived from the 3' primer set is used to quantitate the RNA in the FFPE tissue scrape. The ratio of the RNA yield obtained from both sets of PCR primers is the 3'⁄5' ratio and is used as an indication of RNA quality. If there is less product yield of the 5’ β-actin primers, it indicates that there is less full length of RNA.

I am getting poor quality of RNA from my LCM samples. How can I improve it?

The dissection and embedding steps are critical for the RNA quality for LCM samples. We suggest using the entire adjacent section or dissecting a large area of the section (>1000 cells) for RNA isolation. Before proceeding to LCM microdissection, we recommend assessing the quality of RNA isolated from the adjacent section. With good amount of RNA, one can assess the RNA quantity and quality using the Qubit RNA HS Assay Kit and the Agilent Bioanalyzer. This will help to make sure that good quality RNA is obtained from a low number of LCM cells.

What samples can be used with the Arcuturus PicoPure RNA Isolation Kit?

The Arcturus PicoPure RNA Isolation Kit removes total cellular RNA from pico-scale samples including frozen sections, alcohol fixed-, or fresh samples. Please note, samples fixed with formaldehyde are not recommended for use with this kit.