Arcturus™ Paradise™ Plus stain, slide jars & slides
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Applied Biosystems™

Arcturus™ Paradise™ Plus stain, slide jars & slides

The ARCTURUS™ Paradise™ PLUS FFPE LCM Staining Kit offers a simple solution developed to stain formalin-fixed paraffin-embedded (FFPE) tissues beforeRead more
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Catalog NumberQuantity
KIT0312J24 samples
Catalog number KIT0312J
Price (CNY)
-
Quantity:
24 samples
The ARCTURUS™ Paradise™ PLUS FFPE LCM Staining Kit offers a simple solution developed to stain formalin-fixed paraffin-embedded (FFPE) tissues before proceeding with laser capture microdissection (LCM) and downstream molecular analysis. FFPE samples introduce unique challenges for gene expression profiling, including chemical modification and fragmentation of RNA molecules. Archival of FFPE samples adds to these challenges through increased RNA degradation over time.

Key product features:
• Provide Superior Staining while Preserving RNA
• Secure Downstream Success with the Paradise™ QC Kit
• Benefit from the Only Complete System for Formalin-fixed Samples

Provide Superior Staining while Preserving RNA
The Paradise™ PLUS FFPE LCM Staining Kit offers a simple solution developed to stain FFPE tissues before proceeding with laser microdissection and downstream molecular analysis. It is a fast penetrating stain that provides good contrast by differential staining of nuclei (purple) and cytoplasm (light pink). By minimizing the exposure of tissues to water where nucleases may be activated, the Paradise™ PLUS FFPE LCM Staining Kit helps to preserve RNA integrity that may be otherwise compromised when using longer staining protocols.

Secure Downstream Success with the Paradise™ QC Kit
Identify the best samples for your studies using the Paradise™ PLUS Sample Quality Assessment Kit (QC Kit). The QC Kit uses a simple, real-time PCR technique to check the quality of RNA in your FFPE sample before staining and LCM. Proceed with confidence knowing your FFPE samples have high quality RNA.

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise™ PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from FFPE tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise™ PLUS Reagent System is validated as part of the ARCTURUS™ System for Microgenomics, an integrated solution for utilizing small quantities of RNA for gene expression analysis. ARCTURUS™ Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular details otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Laser Capture Microdissection (LCM)
For Use With (Equipment)ArcturusXT™ LCM Instrument
No. of Samples24 samples
Product LineARCTURUS™, Paradise™
Quantity24 samples
Workflow StepTissue Staining
Sample TypeTissue (FFPE)
Unit SizeEach
Contents & Storage
Paradise stain, staining jars and LCM slides. Store at room temperature

Frequently asked questions (FAQs)

How do I locate the overview image on the Arcturus XT LCM Instrument?

First, optimize the visualization of the image in the live video at 2X by increasing or decreasing the brightness setting, then right-click on the overview image and select "remember settings". This resets your optimized image settings, and when you select "re-acquire overview image" you will now generate a perfectly exposed overview image.

With the ArcturusXT LCM Instrument, which laser should I use to isolate my region of interest? UV cutting laser or IR capture laser?

For a few cells: The gentle IR-only approach is the best recommendation for preserving nucleic acids and verifying that the desired material is collected on the cap. For groups of cells or large tumor regions: Mount your tissue on a membrane slide and take advantage of the power of the IR, or IR + UV cutting laser to cut around your region of interest quickly and cleanly.

What is Arcturus Laser Capture Microdissection (LCM)?

The ArcturusXT LCM Instrument has a proprietary combination of a gentle IR laser and a powerful UV laser that work in conjunction to efficiently isolate cells from frozen sections or paraffin embedded sections without changing morphology or integrity of the biological content. The IR laser helps to capture the cells of interest, and the UV laser microdissects the captured cells. This is done without affecting the morphology of the cells, and allows for visual inspection of the remaining specimen to help ensure the quality of the sample collected. In addition to the flexibility that the dual laser system provides, there is the flexibility of multiple stage inserts for various sample types. A wide-slide stage format is available for neurobiology researchers working with very large sections of brain tissue. The petri dish stage insert enables live-cell microdissection applications such as stem cell research and other rare-cell isolations.