Cross-talk between cytokines and renin-angiotensin in hypothalamic paraventricular nucleus in heart failure: role of nuclear factor-kappaB.
AuthorsKang YM, Ma Y, Elks C, Zheng JP, Yang ZM, Francis J,
JournalCardiovasc Res
PubMed ID18469338
'AIMS: Nuclear factor-kappa B (NF-kappaB) is a potent inducer of pro-inflammatory cytokines (PIC) and oxidative stress in cardiovascular disease. In this study, we determined whether upregulation of NF-kappaB in the hypothalamic paraventricular nucleus (PVN) contributed to neurohumoral excitation either directly, or via interaction with the renin-angiotensin system (RAS), in heart ... More
IL-4 adenoviral gene therapy reduces inflammation, proinflammatory cytokines, vascularization, and bony destruction in rat adjuvant-induced arthritis.
'IL-4 is a cytokine with anti-inflammatory properties on activated macrophages. Rheumatoid arthritis, an autoimmune inflammatory disease, is characterized by a paucity of IL-4 and an abundance of synovial macrophage-derived mediators. Herein, the effect of a single injection of adenovirus-producing rat IL-4 (AxCAIL-4) or a control virus with no inserted gene ... More
Anti-type V collagen humoral immunity in lung transplant primary graft dysfunction.
AuthorsIwata T, Philipovskiy A, Fisher AJ, Presson RG, Chiyo M, Lee J, Mickler E, Smith GN, Petrache I, Brand DB, Burlingham WJ, Gopalakrishnan B, Greenspan DS, Christie JD, Wilkes DS,
JournalJ Immunol
PubMed ID18832733
'Primary graft dysfunction (PGD) is a major complication following lung transplantation. We reported that anti-type V collagen (col(V)) T cell immunity was strongly associated with PGD. However, the role of preformed anti-col(V) Abs and their potential target in PGD are unknown. Col(V) immune serum, purified IgG or B cells from ... More
A novel, potent dual inhibitor of the leukocyte proteases cathepsin G and chymase: molecular mechanisms and anti-inflammatory activity in vivo.
Authorsde Garavilla L, Greco MN, Sukumar N, Chen ZW, Pineda AO, Mathews FS, Di Cera E, Giardino EC, Wells GI, Haertlein BJ, Kauffman JA, Corcoran TW, Derian CK, Eckardt AJ, Damiano BP, Andrade-Gordon P, Maryanoff BE,
JournalJ Biol Chem
PubMed ID15741158
'Certain leukocytes release serine proteases that sustain inflammatory processes and cause disease conditions, such as asthma and chronic obstructive pulmonary disease. We identified beta-ketophosphonate 1 (JNJ-10311795; RWJ-355871) as a novel, potent dual inhibitor of neutrophil cathepsin G (K(i) = 38 nm) and mast cell chymase (K(i) = 2.3 nm). The ... More
Effects of inhaled ambient particulate matter on pulmonary antimicrobial immune defense.
AuthorsZelikoff JT, Chen LC, Cohen MD, Fang K, Gordon T, Li Y, Nadziejko C, Schlesinger RB
JournalInhal Toxicol
PubMed ID12528043
'Respiratory-tract infection, specifically pneumonia, contributes substantially to the increased morbidity and mortality among elderly individuals exposed to airborne particulate matter of <10 microm diameter (PM(10)). These epidemiological findings suggest that PM(10) may act as an immunosuppressive factor that can undermine normal pulmonary antimicrobial defense mechanisms. To investigate whether, and how, ... More
Chronic NF-{kappa}B blockade reduces cytosolic and mitochondrial oxidative stress and attenuates renal injury and hypertension in SHR.
AuthorsElks CM, Mariappan N, Haque M, Guggilam A, Majid DS, Francis J,
JournalAm J Physiol Renal Physiol
PubMed ID19073636
'Nuclear factor-kappaB (NF-kappaB) plays an important role in hypertensive renal injury; however, its roles in perpetuating mitochondrial oxidative stress and renal dysfunction remain unclear. In this study, we assessed the effects of chronic NF-kappaB blockade with pyrrolidine dithiocarbamate (PDTC) on renal dysfunction and mitochondrial redox status in spontaneously hypertensive rats ... More
Effect of allergen sensitization on external root resorption.
AuthorsMurata N, Ioi H, Ouchi M, Takao T, Oida H, Aijima R, Yamaza T, Kido MA,
JournalJ Dent Res
PubMed ID23648742
'In orthodontic tooth movement (OTM), we should be concerned about external root resorption (ERR) as an undesirable iatrogenic problem, but its mechanisms are not fully understood. Since our previous epidemiologic studies found that patients with allergic diseases showed higher rates of ERR during orthodontic treatment, we explored the possible effect ... More
Thymosin alpha 1 attenuates lipid peroxidation and improves fructose-induced steatohepatitis in rats.
AuthorsArmutcu F, Coskun O, Gürel A, Kanter M, Can M, Ucar F, Unalacak M,
JournalClin Biochem
PubMed ID15885234
'The aim of this study was to investigate the effects of thymosin alpha(1) (Talpha(1)) in rats having fructose-induced steatosis. Fructose leads to experimental steatosis in the liver by exerting its effect on some components of the oxidant/antioxidant system, and on several cytokines (interleukin-1beta, -2, and -6) in blood. Twenty-four rats ... More
Gut-derived norepinephrine plays an important role in up-regulating IL-1beta and IL-10.
AuthorsZhou M, Das P, Simms HH, Wang P
JournalBiochim Biophys Acta
PubMed ID15949713
'Previous studies have shown that the gut is a major source of norepinephrine (NE) released in early sepsis and that gut-derived NE plays an important role in up-regulating TNF-alpha expression in Kupffer cells (KC) via an alpha(2)-adrenoceptor (alpha(2)-AR) pathway. However, it remains unknown whether NE affects the release of other ... More
Macrophage colony-stimulating factor and its receptor signaling augment glycated albumin-induced retinal microglial inflammation in vitro.
AuthorsLiu W, Xu GZ, Jiang CH, Tian J
JournalBMC Cell Biol
PubMed ID21266043
Microglial activation and the proinflammatory response are controlled by a complex regulatory network. Among the various candidates, macrophage colony-stimulating factor (M-CSF) is considered an important cytokine. The up-regulation of M-CSF and its receptor CSF-1R has been reported in brain disease, as well as in diabetic complications; however, the mechanism is ... More
Chemokine-cytokine cross-talk. The ELR+ CXC chemokine LIX (CXCL5) amplifies a proinflammatory cytokine response via a phosphatidylinositol 3-kinase-NF-kappa B pathway.
AuthorsChandrasekar B, Melby PC, Sarau HM, Raveendran M, Perla RP, Marelli-Berg FM, Dulin NO, Singh IS,
JournalJ Biol Chem
PubMed ID12468547
It is well established that cytokines can induce the production of chemokines, but the role of chemokines in the regulation of cytokine expression has not been fully investigated. Exposure of rat cardiac-derived endothelial cells (CDEC) to lipopolysaccharide-induced CXC chemokine (LIX), and to a lesser extent to KC and MIP-2, activated ... More
Systemic inflammation induced by lipopolysaccharide increases neointimal formation after balloon and stent injury in rabbits.
AuthorsDanenberg HD, Welt FG, Walker M, Seifert P, Toegel GS, Edelman ER,
JournalCirculation
PubMed ID12070123
BACKGROUND: Emerging data indicate that the inflammatory response after mechanical arterial injury correlates with the severity of neointimal hyperplasia in animal models and postangioplasty restenosis in humans. The present study was designed to examine whether a nonspecific stimulation of the innate immune system, induced in close temporal proximity to the ... More
Whey protein enhances normal inflammatory responses during cutaneous wound healing in diabetic rats.
AuthorsEbaid H, Salem A, Sayed A, Metwalli A,
JournalLipids Health Dis
PubMed ID22168406
Prolonged wound healing is a complication of diabetes that contributes to mortality. Impaired wound healing occurs as a consequence of excessive reactive oxygen species (ROS) production. Whey protein (WP) is able to reduce the oxygen radicals and increase the levels of the antioxidant glutathione. Thus, the aim of this study ... More
Transfection with a dominant-negative inhibitor of monocyte chemoattractant protein-1 gene improves cardiac function after 6 hours of cold preservation.
AuthorsKajihara N, Morita S, Nishida T, Tatewaki H, Eto M, Egashira K, Yasui H,
JournalCirculation
PubMed ID12970235
Monocyte chemoattractant protein-1 (MCP-1), a potent chemotactic factor for monocytes, is induced during ischemia-reperfusion. As monocytes might play an important causative role in reperfusion injury, we investigated if inhibition of monocyte activation could attenuate ischemia-reperfusion injury and thereby improve cardiac preservation. To inhibit monocyte activation, we transfected a dominant-negative inhibitor ... More
Differential gene expression profiling in whole blood during acute systemic inflammation in lipopolysaccharide-treated rats.
AuthorsFannin RD, Auman JT, Bruno ME, Sieber SO, Ward SM, Tucker CJ, Merrick BA, Paules RS,
JournalPhysiol Genomics
PubMed ID15781589
Microarrays have been used to evaluate the expression of thousands of genes in various tissues. However, few studies have investigated the change in gene expression profiles in one of the most easily accessible tissues, whole blood. We utilized an acute inflammation model to investigate the possibility of using a cDNA ... More
Postischemic gene transfer of interleukin-10 protects against both focal and global brain ischemia.
AuthorsOoboshi H, Ibayashi S, Shichita T, Kumai Y, Takada J, Ago T, Arakawa S, Sugimori H, Kamouchi M, Kitazono T, Iida M,
JournalCirculation
PubMed ID15710762
Gene therapy may be a promising approach for treatment of brain ischemia, although the efficiency of postischemic gene therapy is not established. Our goal in this study was to examine the effects of gene transfer of interleukin-10 (IL-10), an antiinflammatory cytokine, after induction of brain ischemia. Brain ischemia was produced ... More
Effect of ventilator-induced lung injury on the development of reperfusion injury in a rat lung transplant model.
Authorsde Perrot M, Imai Y, Volgyesi GA, Waddell TK, Liu M, Mullen JB, McRae K, Zhang H, Slutsky AS, Ranieri VM, Keshavjee S,
JournalJ Thorac Cardiovasc Surg
PubMed ID12447179
Although mechanical ventilation can potentially worsen preexisting lung injury, its importance in the setting of lung transplantation has not been explored. This study was undertaken to examine the effect of 2 ventilatory strategies on the development of ischemia-reperfusion injury after lung transplantation. In a rat lung transplant model animals were ... More
Elevation of urinary IL-1beta levels during transplant associated nephropathy in rat model of the nephrotic syndrome.
AuthorsHiguchi A, Fujisawa M, Ueno K, Kamidono S,
JournalUrol Res
PubMed ID12111178
A recurrence of nephrotic syndrome is a well-known phenomenon in patients who receive kidney transplantation. In this study, we attempt to establish a rat model of recurrent nephrotic change after renal transplantation using puromycin aminonucleoside (PA) induced nephrotic rats. We then examine the mechanism leading to recurrence. Female Sprague-Dawley rats ... More
Prenatal exposure to maternal infection alters cytokine expression in the placenta, amniotic fluid, and fetal brain.
AuthorsUrakubo A, Jarskog LF, Lieberman JA, Gilmore JH,
JournalSchizophr Res
PubMed ID11163542
Prenatal exposure to infection appears to increase the risk of schizophrenia and other neurodevelopmental disorders. We have hypothesized that cytokines, generated in response to maternal infection, play a key mechanistic role in this association. E16 timed pregnancy rats were injected i.p. with Escherichia coli lipopolysaccharide (LPS) to model prenatal exposure ... More
Mechanism of the beneficial effects of pentoxifylline during sepsis: maintenance of adrenomedullin responsiveness and downregulation of proinflammatory cytokines.
AuthorsKoo DJ, Yoo P, Cioffi WG, Bland KI, Chaudry IH, Wang P,
JournalJ Surg Res
PubMed ID10816353
Although it is known that pentoxifylline (PTX) produces various beneficial effects during sepsis, it remains unknown whether this agent has any salutary effects on the depressed vascular responsiveness to adrenomedullin (ADM), a novel potent vasodilatory peptide, under such conditions. Adult male Sprague-Dawley rats were subjected to polymicrobial sepsis by cecal ... More
Expression and function of the C5a receptor in rat alveolar epithelial cells.
AuthorsRiedemann NC, Guo RF, Sarma VJ, Laudes IJ, Huber-Lang M, Warner RL, Albrecht EA, Speyer CL, Ward PA,
JournalJ Immunol
PubMed ID11823527
Although alveolar epithelial cells (AEC) form an important barrier for host defenses in the lung, there is limited information about ways in which AEC can directly participate in the lung inflammatory response. In the current studies, primary cultures of rat AEC (RAEC) have been shown to specifically bind recombinant rat ... More
Microglial activation and TNFalpha production mediate altered CNS excitability following peripheral inflammation.
AuthorsRiazi K, Galic MA, Kuzmiski JB, Ho W, Sharkey KA, Pittman QJ,
JournalProc Natl Acad Sci U S A
PubMed ID18955701
Peripheral inflammation leads to a number of centrally mediated physiological and behavioral changes. The underlying mechanisms and the signaling pathways involved in these phenomena are not yet well understood. We hypothesized that peripheral inflammation leads to increased neuronal excitability arising from a CNS immune response. We induced inflammation in the ... More
Cytomegalovirus infection of vascular cells induces expression of pro-inflammatory adhesion molecules by paracrine action of secreted interleukin-1beta.
AuthorsDengler TJ, Raftery MJ, Werle M, Zimmermann R, Schönrich G,
JournalTransplantation
PubMed ID10762222
Infection with human cytomegalovirus (HCMV) has been associated with vascular disease processes such as vascular allograft rejection, transplantation vasculopathy, restenosis after angioplasty, and native atherosclerosis. To elucidate underlying pathomechanisms, the effect of acute HCMV infection on the expression of pro-inflammatory adhesion molecules on human umbilical vein endothelial cells (HUVEC) and ... More
Inhibition of caspase-1-like activity by Ac-Tyr-Val-Ala-Asp-chloromethyl ketone induces long-lasting neuroprotection in cerebral ischemia through apoptosis reduction and decrease of proinflammatory cytokines.
AuthorsRabuffetti M, Sciorati C, Tarozzo G, Clementi E, Manfredi AA, Beltramo M,
JournalJ Neurosci
PubMed ID10844008
Broad spectrum caspase inhibitors have been found to reduce neurodegeneration caused by cerebral ischemia. We studied whether blockade of group I caspases, mainly caspase-1, using the inhibitor Ac-YVAD.cmk reduced infarct volume and produced prolonged neuroprotection. Ac-YVAD.cmk (300 ng/rat) was injected intracerebroventricularly 10 min after permanent middle cerebral artery occlusion in ... More
Microsomal cytochrome P450 levels and activities of isolated rat livers perfused with albumin.
AuthorsVuppugalla R, Shah RB, Chimalakonda AP, Fisher CW, Mehvar R,
JournalPharm Res
PubMed ID12608540
We recently showed that the perfusion of isolated rat livers with perfusates containing bovine serum albumin (BSA) would significantly stimulate the release of tumor necrosis factor (TNF)-alpha. Here, we hypothesize that BSA-induced increase in the release of TNF-alpha, and possibly other cytokines, would affect cytochrome P450 (CYP)-mediated drug metabolism. Rat ... More
Prevention of the onset and progression of collagen-induced arthritis in rats by the potent p38 mitogen-activated protein kinase inhibitor FR167653.
AuthorsNishikawa M, Myoui A, Tomita T, Takahi K, Nampei A, Yoshikawa H,
JournalArthritis Rheum
PubMed ID13130488
FR167653 is a potent inhibitor of p38 mitogen-activated protein kinase (MAPK) and inhibits tumor necrosis factor alpha (TNFalpha) and interleukin-1 beta (IL-1 beta) production in inflammatory cells. In this study we investigated the effect of FR167653 on collagen-induced arthritis (CIA). Rats with CIA were subcutaneously injected with FR167653 (32 mg/kg/day) ... More
Estrogen treatment following severe burn injury reduces brain inflammation and apoptotic signaling.
Patients with severe burn injury experience a rapid elevation in multiple circulating pro-inflammatory cytokines, with the levels correlating with both injury severity and outcome. Accumulations of these cytokines in animal models have been observed in remote organs, however data are lacking regarding early brain cytokine levels following burn injury, and ... More
Autocrine loop between TGF-beta1 and IL-1beta through Smad3- and ERK-dependent pathways in rat pancreatic stellate cells.
AuthorsAoki H, Ohnishi H, Hama K, Ishijima T, Satoh Y, Hanatsuka K, Ohashi A, Wada S, Miyata T, Kita H, Yamamoto H, Osawa H, Sato K, Tamada K, Yasuda H, Mashima H, Sugano K
JournalAm J Physiol Cell Physiol
PubMed ID16371439
Pancreatic stellate cells (PSCs) are activated during pancreatitis and promote pancreatic fibrosis by producing and secreting ECMs such as collagen and fibronectin. IL-1beta has been assumed to participate in pancreatic fibrosis by activating PSCs. Activated PSCs secrete various cytokines that regulate PSC function. In this study, we have examined IL-1beta ... More
Specific inhibition of mitochondrial oxidative stress suppresses inflammation and improves cardiac function in a rat pneumonia-related sepsis model.
AuthorsZang QS, Sadek H, Maass DL, Martinez B, Ma L, Kilgore JA, Williams NS, Frantz DE, Wigginton JG, Nwariaku FE, Wolf SE, Minei JP
JournalAm J Physiol Heart Circ Physiol
PubMed ID22408027
Using a mitochondria-targeted vitamin E (Mito-Vit-E) in a rat pneumonia-related sepsis model, we examined the role of mitochondrial reactive oxygen species in sepsis-mediated myocardial inflammation and subsequent cardiac contractile dysfunction. Sepsis was produced in adult male Sprague-Dawley rats via intratracheal injection of S. pneumonia (4 × 10(6) colony formation units ... More
Gender differences in oesophageal mucosal injury in a reflux oesophagitis model of rats.
AuthorsMasaka T, Iijima K, Endo H, Asanuma K, Ara N, Ishiyama F, Asano N, Koike T, Imatani A, Shimosegawa T
JournalGut
PubMed ID22287598
ObjectiveThere is a strong male predominance of oesophageal adenocarcinoma, which might be related to the higher prevalence of precursor lesions such as erosive reflux oesophagitis in men compared with women. This experiment investigated the gender difference in a reflux oesophagitis model of rats and explored the potential role of oestrogen ... More
Inhibition of IL-18 reduces myeloperoxidase activity and prevents edema in intestine following alcohol and burn injury.
AuthorsRana SN, Li X, Chaudry IH, Bland KI, Choudhry MA
JournalJ Leukoc Biol
PubMed ID15728717
Previous studies have shown that alcohol (EtOH) ingestion before burn injury impaired intestinal barrier and immune function. This study determined whether EtOH and burn injury up-regulate interleukin (IL)-18 and whether IL-18 up-regulation following EtOH and burn injury is a cause for neutrophil recruitment and increased intestinal edema. Rats (250 g) ... More