Regulatory functions of self-restricted MHC class II allopeptide-specific Th2 clones in vivo.
AuthorsWaaga AM, Gasser M, Kist-van Holthe JE, Najafian N, Müller A, Vella JP, Womer KL, Chandraker A, Khoury SJ, Sayegh MH,
JournalJ Clin Invest
PubMed ID11285310
'We studied T-cell clones generated from grafts of rejecting and tolerant animals and investigated the regulatory function of Th2 clones in vitro and in vivo. To prevent allograft rejection, we treated LEW strain recipient rats of WF strain kidney grafts with CTLA4Ig to block CD28-B7 costimulation. We then isolated epitope-specific ... More
Corticosterone suppresses mesenteric lymph node T cells by inhibiting p38/ERK pathway and promotes bacterial translocation after alcohol and burn injury.
'Previous studies showed that alcohol (EtOH) intoxication before burn injury suppresses mesenteric lymph node (MLN) T cell functions and increases gut bacterial translocation. In this study, we examined whether corticosterone (Cort) plays any role in suppressing MLN T cell function and bacterial accumulation after EtOH intoxication and burn injury. Rats ... More
Thymosin alpha 1 attenuates lipid peroxidation and improves fructose-induced steatohepatitis in rats.
AuthorsArmutcu F, Coskun O, Gürel A, Kanter M, Can M, Ucar F, Unalacak M,
JournalClin Biochem
PubMed ID15885234
'The aim of this study was to investigate the effects of thymosin alpha(1) (Talpha(1)) in rats having fructose-induced steatosis. Fructose leads to experimental steatosis in the liver by exerting its effect on some components of the oxidant/antioxidant system, and on several cytokines (interleukin-1beta, -2, and -6) in blood. Twenty-four rats ... More
NK cells inhibit T cell proliferation via p21-mediated cell cycle arrest.
AuthorsTrivedi PP, Roberts PC, Wolf NA, Swanborg RH,
JournalJ Immunol
PubMed ID15814681
'NK cells have been shown to influence immune responses via direct interaction with cells of the adaptive immune system, such as dendritic cells, B cells, and T cells. A role for NK cells in down-regulation of T cell responses has been implicated in several studies; however, the underlying mechanism of ... More
Quantitative in vivo cytokine analysis at synthetic biomaterial implant sites.
AuthorsRodriguez A, Meyerson H, Anderson JM,
JournalJ Biomed Mater Res A
PubMed ID18431759
To further elucidate the foreign body reaction, investigation of cytokines at biomaterial implant sites was carried out using a multiplex immunoassay and ELISA. Macrophage activation cytokines (IL-1beta, IL-6, and TNFalpha), cytokines important for macrophage fusion (IL-4 and IL-13), antiinflammatory cytokines (IL-10 and TGFbeta), chemokines (GRO/KC, MCP-1), and the T-cell activation ... More
Suppression of gamma interferon transcription and production by nematode excretory-secretory antigen during polyclonal stimulation of rat lymph node T cells.
AuthorsUchikawa R, Matsuda S, Arizono N,
JournalInfect Immun
PubMed ID11035730
Although certain helminth infections preferentially induce type 2 T-cell responses, the immunological mechanisms responsible for type 2 T-cell polarization remain unclear. In the present study, we investigated the effects of excretory-secretory (ES) antigen from the nematode Nippostrongylus brasiliensis on cytokine production by mesenteric lymph node (MLN) cells isolated from naive ... More
Calcineurin inhibition in splenocytes induced by pavlovian conditioning.
AuthorsPacheco-López G, Riether C, Doenlen R, Engler H, Niemi MB, Engler A, Kavelaars A, Heijnen CJ, Schedlowski M,
JournalFASEB J
PubMed ID19103649
Pavlovian conditioning is one of the major neurobiological mechanisms of placebo effects, potentially influencing the course of specific diseases and the response to a pharmacological therapy, such as immunosuppression. In our study with behaviorally conditioned rats, a relevant taste (0.2% saccharin) preceded the application of the immunosuppressive drug cyclosporin A ... More
Crucial role of CCL3/MIP-1alpha in the recurrence of autoimmune anterior uveitis induced with myelin basic protein in Lewis rats.
AuthorsManczak M, Jiang S, Orzechowska B, Adamus G,
JournalJ Autoimmun
PubMed ID12144807
In this study, we examined the role of CCL2/MCP-1, CCL3/MIP-1alpha, CCL4/MIP-1beta, and CCL5/RANTES during recurrent anterior uveitis (RAU). LEW rats injected with myelin basic protein (MBP) developed experimental autoimmune encephalomyelitis (EAE) and associated anterior uveitis (AU), which was mediated by CD4(+) T cells. After recovery, rats become resistant to EAE ... More
Elevation of urinary IL-1beta levels during transplant associated nephropathy in rat model of the nephrotic syndrome.
AuthorsHiguchi A, Fujisawa M, Ueno K, Kamidono S,
JournalUrol Res
PubMed ID12111178
A recurrence of nephrotic syndrome is a well-known phenomenon in patients who receive kidney transplantation. In this study, we attempt to establish a rat model of recurrent nephrotic change after renal transplantation using puromycin aminonucleoside (PA) induced nephrotic rats. We then examine the mechanism leading to recurrence. Female Sprague-Dawley rats ... More
Clinical assessment of blood leukocytes, serum cytokines, and serum immunoglobulins as responses to sleep deprivation in laboratory rats.
AuthorsEverson CA,
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID15947073
The specific systems and mechanisms affected by sleep deprivation that may perpetuate disease processes in humans still are speculative. In laboratory rats, prolonged sleep deprivation induces a state marked by abnormal control over indigenous bacteria that results in transient infections of internal tissues and eventual lethal septicemia. The present studies ... More