Suppressive DNA vaccination in myelin oligodendrocyte glycoprotein peptide-induced experimental autoimmune encephalomyelitis involves a T1-biased immune response.
AuthorsLobell A, Weissert R, Eltayeb S, de Graaf KL, Wefer J, Storch MK, Lassmann H, Wigzell H, Olsson T,
JournalJ Immunol
PubMed ID12574345
'Vaccination with DNA encoding a myelin basic protein peptide suppresses Lewis rat experimental autoimmune encephalomyelitis (EAE) induced with the same peptide. Additional myelin proteins, such as myelin oligodendrocyte glycoprotein (MOG), may be important in multiple sclerosis. Here we demonstrate that DNA vaccination also suppresses MOG peptide-induced EAE. MOG(91-108) is encephalitogenic ... More
Regulatory functions of self-restricted MHC class II allopeptide-specific Th2 clones in vivo.
AuthorsWaaga AM, Gasser M, Kist-van Holthe JE, Najafian N, Müller A, Vella JP, Womer KL, Chandraker A, Khoury SJ, Sayegh MH,
JournalJ Clin Invest
PubMed ID11285310
'We studied T-cell clones generated from grafts of rejecting and tolerant animals and investigated the regulatory function of Th2 clones in vitro and in vivo. To prevent allograft rejection, we treated LEW strain recipient rats of WF strain kidney grafts with CTLA4Ig to block CD28-B7 costimulation. We then isolated epitope-specific ... More
Pertussis toxin alters the innate and the adaptive immune responses in a pertussis-dependent model of autoimmunity.
AuthorsAgarwal RK, Sun SH, Su SB, Chan CC, Caspi RR,
JournalJ Neuroimmunol
PubMed ID12161029
'Pertussis toxin (PTX) is used to promote development of autoimmune diseases. The mechanism(s) are still incompletely understood. We dissected the innate and adaptive immune responses in a PTX-dependent model of autoimmune retinal disease, experimental autoimmune uveoretinitis (EAU), a Th1-driven disease of the neural retina elicited in F344 rats with a ... More
The effect of treatment with a protease inhibitor on mycobacterial infection.
AuthorsKabbesh M, Koomer A, Molteni A, Amajoyi R, Quinn T, Herndon B,
JournalDiagn Microbiol Infect Dis
PubMed ID15808316
'Mycobacterial infection occurs frequently in patients that receive protease inhibitors, which are drugs used to treat AIDS, but are known for metabolic effects. Proteases of microbial antigens have been recognized as important regulators of host inflammation and cellular response. To evaluate protease inhibitor effect on a mycobacterial infection, a pilot ... More
MHC class II-regulated central nervous system autoaggression and T cell responses in peripheral lymphoid tissues are dissociated in myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis.
We dissected the requirements for disease induction of myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis in MHC (RT1 in rat) congenic rats with overlapping MOG peptides. Immunodominance with regard to peptide-specific T cell responses was purely MHC class II dependent, varied between different MHC haplotypes, and was linked to encephalitogenicity ... More
Ficus carica Leaf Extract Modulates the Lipid Profile of Rats Fed with a High-Fat Diet through an Increase of HDL-C.
AuthorsJoerin L, Kauschka M, Bonnländer B, Pischel I, Benedek B, Butterweck V,
JournalPhytother Res
PubMed ID23606376
Ficus carica has been traditionally used for the treatment of several metabolic syndrome-related health problems. It was the objective of this study to investigate the preventive effects of a Ficus carica (FC) leaf extract on hyperlipidemia in high fat diet (HFD)-induced obese male rats. Male Sprague-Dawley rats (180 - 200 ... More
BB rat thymocytes cultured in the presence of islets lose their ability to transfer autoimmune diabetes.
Thymocytes from adult BB rats can adoptively transfer autoimmune diabetes to athymic recipients. It is also known that the development of BB rat T-cells is recapitulated in adult thymus organ cultures (ATOCs). Based on these observations, we tested the hypothesis that cells capable of the adoptive transfer of diabetes would ... More
Protective DNA vaccination against organ-specific autoimmunity is highly specific and discriminates between single amino acid substitutions in the peptide autoantigen.
AuthorsWeissert R, Lobell A, de Graaf KL, Eltayeb SY, Andersson R, Olsson T, Wigzell H,
JournalProc Natl Acad Sci U S A
PubMed ID10677519
DNA vaccines that encode encephalitogenic sequences in tandem can protect from subsequent experimental autoimmune encephalomyelitis induced with the corresponding peptide. The mechanism for this protection and, in particular, if it is specific for the amino acid sequence encoding the vaccine are not known. We show here that a single amino ... More
Crucial role of CCL3/MIP-1alpha in the recurrence of autoimmune anterior uveitis induced with myelin basic protein in Lewis rats.
AuthorsManczak M, Jiang S, Orzechowska B, Adamus G,
JournalJ Autoimmun
PubMed ID12144807
In this study, we examined the role of CCL2/MCP-1, CCL3/MIP-1alpha, CCL4/MIP-1beta, and CCL5/RANTES during recurrent anterior uveitis (RAU). LEW rats injected with myelin basic protein (MBP) developed experimental autoimmune encephalomyelitis (EAE) and associated anterior uveitis (AU), which was mediated by CD4(+) T cells. After recovery, rats become resistant to EAE ... More
Prevention of the onset and progression of collagen-induced arthritis in rats by the potent p38 mitogen-activated protein kinase inhibitor FR167653.
AuthorsNishikawa M, Myoui A, Tomita T, Takahi K, Nampei A, Yoshikawa H,
JournalArthritis Rheum
PubMed ID13130488
FR167653 is a potent inhibitor of p38 mitogen-activated protein kinase (MAPK) and inhibits tumor necrosis factor alpha (TNFalpha) and interleukin-1 beta (IL-1 beta) production in inflammatory cells. In this study we investigated the effect of FR167653 on collagen-induced arthritis (CIA). Rats with CIA were subcutaneously injected with FR167653 (32 mg/kg/day) ... More
Clinical assessment of blood leukocytes, serum cytokines, and serum immunoglobulins as responses to sleep deprivation in laboratory rats.
AuthorsEverson CA,
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID15947073
The specific systems and mechanisms affected by sleep deprivation that may perpetuate disease processes in humans still are speculative. In laboratory rats, prolonged sleep deprivation induces a state marked by abnormal control over indigenous bacteria that results in transient infections of internal tissues and eventual lethal septicemia. The present studies ... More