Sulfatide, a major lipid component of myelin sheath, activates inflammatory responses as an endogenous stimulator in brain-resident immune cells.
AuthorsJeon SB, Yoon HJ, Park SH, Kim IH, Park EJ,
JournalJ Immunol
PubMed ID19018000
'Sulfatide, a major lipid component of myelin sheath, participates in diverse cellular events of the CNS, and its cellular level has recently been implicated in many inflammation-associated neuronal diseases. Herein, we report that sulfatide alone can trigger pathological inflammatory responses in glia, brain-resident immune cells. We show that sulfatide changed ... More
Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve.
AuthorsDubový P, Brázda V, Klusáková I, Hradilová-Svíženská I,
JournalJ Neuroinflammation
PubMed ID23634725
'Current research implicates interleukin (IL)-6 as a key component of the nervous-system response to injury with various effects. We used unilateral chronic constriction injury (CCI) of rat sciatic nerve as a model for neuropathic pain. Immunofluorescence, ELISA, western blotting and in situ hybridization were used to investigate bilateral changes in ... More
Cross-talk between cytokines and renin-angiotensin in hypothalamic paraventricular nucleus in heart failure: role of nuclear factor-kappaB.
AuthorsKang YM, Ma Y, Elks C, Zheng JP, Yang ZM, Francis J,
JournalCardiovasc Res
PubMed ID18469338
'AIMS: Nuclear factor-kappa B (NF-kappaB) is a potent inducer of pro-inflammatory cytokines (PIC) and oxidative stress in cardiovascular disease. In this study, we determined whether upregulation of NF-kappaB in the hypothalamic paraventricular nucleus (PVN) contributed to neurohumoral excitation either directly, or via interaction with the renin-angiotensin system (RAS), in heart ... More
Pertussis toxin alters the innate and the adaptive immune responses in a pertussis-dependent model of autoimmunity.
AuthorsAgarwal RK, Sun SH, Su SB, Chan CC, Caspi RR,
JournalJ Neuroimmunol
PubMed ID12161029
'Pertussis toxin (PTX) is used to promote development of autoimmune diseases. The mechanism(s) are still incompletely understood. We dissected the innate and adaptive immune responses in a PTX-dependent model of autoimmune retinal disease, experimental autoimmune uveoretinitis (EAU), a Th1-driven disease of the neural retina elicited in F344 rats with a ... More
Effects of inhaled ambient particulate matter on pulmonary antimicrobial immune defense.
AuthorsZelikoff JT, Chen LC, Cohen MD, Fang K, Gordon T, Li Y, Nadziejko C, Schlesinger RB
JournalInhal Toxicol
PubMed ID12528043
'Respiratory-tract infection, specifically pneumonia, contributes substantially to the increased morbidity and mortality among elderly individuals exposed to airborne particulate matter of <10 microm diameter (PM(10)). These epidemiological findings suggest that PM(10) may act as an immunosuppressive factor that can undermine normal pulmonary antimicrobial defense mechanisms. To investigate whether, and how, ... More
Chronic NF-{kappa}B blockade reduces cytosolic and mitochondrial oxidative stress and attenuates renal injury and hypertension in SHR.
AuthorsElks CM, Mariappan N, Haque M, Guggilam A, Majid DS, Francis J,
JournalAm J Physiol Renal Physiol
PubMed ID19073636
'Nuclear factor-kappaB (NF-kappaB) plays an important role in hypertensive renal injury; however, its roles in perpetuating mitochondrial oxidative stress and renal dysfunction remain unclear. In this study, we assessed the effects of chronic NF-kappaB blockade with pyrrolidine dithiocarbamate (PDTC) on renal dysfunction and mitochondrial redox status in spontaneously hypertensive rats ... More
Angiotensin II AT1 receptor blockade decreases lipopolysaccharide-induced inflammation in the rat adrenal gland.
AuthorsSanchez-Lemus E, Murakami Y, Larrayoz-Roldan IM, Moughamian AJ, Pavel J, Nishioku T, Saavedra JM,
JournalEndocrinology
PubMed ID18556352
'Peripheral administration of bacterial endotoxin [lipopolysaccharide (LPS)] to rodents produces an innate immune response and hypothalamic-pituitary-adrenal axis stimulation. Renin-angiotensin-aldosterone system inhibition by angiotensin II AT1 receptor blockade has antiinflammatory effects in the vasculature. We studied whether angiotensin II receptor blockers (ARBs) prevent the LPS response. We focused on the adrenal ... More
Relationship between production of acute-phase proteins and strength of inflammatory stimulation in rats.
AuthorsKuribayashi T, Tomizawa M, Seita T, Tagata K, Yamamoto S
JournalLab Anim
PubMed ID21669904
'The relationship between intensity of inflammatory stimulation and production of a(2)-macroglobulin (a2M) and a(1)-acid glycoprotein (AAG) in rats was investigated. Sprague-Dawley rats were injected with turpentine oil at doses of 0.05, 0.2 or 0.4 mL/rat. Serum levels of a2M, interleukin (IL)-6 and cytokine-induced neutrophil chemoattractant-1 (CINC-1) were measured by enzyme-linked ... More
Effect of allergen sensitization on external root resorption.
AuthorsMurata N, Ioi H, Ouchi M, Takao T, Oida H, Aijima R, Yamaza T, Kido MA,
JournalJ Dent Res
PubMed ID23648742
'In orthodontic tooth movement (OTM), we should be concerned about external root resorption (ERR) as an undesirable iatrogenic problem, but its mechanisms are not fully understood. Since our previous epidemiologic studies found that patients with allergic diseases showed higher rates of ERR during orthodontic treatment, we explored the possible effect ... More
Thymosin alpha 1 attenuates lipid peroxidation and improves fructose-induced steatohepatitis in rats.
AuthorsArmutcu F, Coskun O, Gürel A, Kanter M, Can M, Ucar F, Unalacak M,
JournalClin Biochem
PubMed ID15885234
'The aim of this study was to investigate the effects of thymosin alpha(1) (Talpha(1)) in rats having fructose-induced steatosis. Fructose leads to experimental steatosis in the liver by exerting its effect on some components of the oxidant/antioxidant system, and on several cytokines (interleukin-1beta, -2, and -6) in blood. Twenty-four rats ... More
Soybean-derived Bowman-Birk inhibitor inhibits neurotoxicity of LPS-activated macrophages.
AuthorsLi J, Ye L, Cook DR, Wang X, Liu J, Kolson DL, Persidsky Y, Ho WZ
JournalJ Neuroinflammation
PubMed ID21324129
Lipopolysaccharide (LPS), the major component of the outer membrane of gram-negative bacteria, can activate immune cells including macrophages. Activation of macrophages in the central nervous system (CNS) contributes to neuronal injury. Bowman-Birk inhibitor (BBI), a soybean-derived protease inhibitor, has anti-inflammatory properties. In this study, we examined whether BBI has the ... More
Intrathecal interleukin-1 receptor antagonist in combination with soluble tumor necrosis factor receptor exhibits an anti-allodynic action in a rat model of neuropathic pain.
AuthorsSweitzer S, Martin D, DeLeo JA,
JournalNeuroscience
PubMed ID11246166
The expression of interleukin-1beta and tumor necrosis factor has previously been shown to be up-regulated in the spinal cord of several rat mononeuropathy models. This present study was undertaken to determine whether blocking the action of central interleukin-1beta and tumor necrosis factor attenuates mechanical allodynia in a gender-specific manner in ... More
Prior infection exacerbates postoperative cognitive dysfunction in aged rats.
AuthorsHovens IB, van Leeuwen BL, Nyakas C, Heineman E, van der Zee EA, Schoemaker RG,
Journal
PubMed ID25972458
Older patients may experience persisting postoperative cognitive dysfunction (POCD), which is considered to largely depend on surgery-induced (neuro)inflammation. We hypothesize that inflammatory events before surgery could predispose patients to POCD. When part of our aged rats developed Mycoplasma pulmonis, this presented the unique opportunity to investigate whether a pulmonary infection ... More
Ventilator-induced coagulopathy in experimental Streptococcus pneumoniae pneumonia.
AuthorsHaitsma JJ, Schultz MJ, Hofstra JJ, Kuiper JW, Juco J, Vaschetto R, Levi M, Zhang H, Slutsky AS,
JournalEur Respir J
PubMed ID18768578
Pneumonia, the main cause of acute lung injury, is characterised by a local pro-inflammatory response and coagulopathy. Mechanical ventilation (MV) is often required. However, MV can lead to additional injury: so-called ventilator-induced lung injury (VILI). Therefore, the current authors investigated the effect of VILI on alveolar fibrin turnover in Streptococcus ... More
Intra-articular injections of high-molecular-weight hyaluronic acid have biphasic effects on joint inflammation and destruction in rat antigen-induced arthritis.
AuthorsRoth A, Mollenhauer J, Wagner A, Fuhrmann R, Straub A, Venbrocks RA, Petrow P, Bräuer R, Schubert H, Ozegowski J, Peschel G, Müller PJ, Kinne RW,
JournalArthritis Res Ther
PubMed ID15899053
To assess the potential use of hyaluronic acid (HA) as adjuvant therapy in rheumatoid arthritis, the anti-inflammatory and chondroprotective effects of HA were analysed in experimental rat antigen-induced arthritis (AIA). Lewis rats with AIA were subjected to short-term (days 1 and 8, n = 10) or long-term (days 1, 8, ... More
Plasma-derived human antithrombin attenuates ventilator-induced coagulopathy but not inflammation in a Streptococcus pneumoniae pneumonia model in rats.
AuthorsAslami H, Haitsma JJ, Hofstra JJ, Florquin S, Dos Santos C, Streutker C, Zhang H, Levi M, Slutsky AS, Schultz MJ,
JournalJ Thromb Haemost
PubMed ID22236057
Mechanical ventilation exaggerates pneumonia-associated pulmonary coagulopathy and inflammation. We hypothesized that the administration of plasma-derived human antithrombin (AT), one of the natural inhibitors of coagulation, prevents ventilator-induced pulmonary coagulopathy, inflammation and bacterial outgrowth in a Streptococcus pneumoniae pneumonia model in rats. Forty-eight hours after induction of S. pneumoniae pneumonia rats ... More
High tidal volume mechanical ventilation-induced lung injury in rats is greater after acid instillation than after sepsis-induced acute lung injury, but does not increase systemic inflammation: an experimental study.
To examine whether acute lung injury from direct and indirect origins differ in susceptibility to ventilator-induced lung injury (VILI) and resultant systemic inflammatory responses. Rats were challenged by acid instillation or 24 h of sepsis induced by cecal ligation and puncture, followed by mechanical ventilation (MV) with either a low ... More
Differential gene expression profiling in whole blood during acute systemic inflammation in lipopolysaccharide-treated rats.
AuthorsFannin RD, Auman JT, Bruno ME, Sieber SO, Ward SM, Tucker CJ, Merrick BA, Paules RS,
JournalPhysiol Genomics
PubMed ID15781589
Microarrays have been used to evaluate the expression of thousands of genes in various tissues. However, few studies have investigated the change in gene expression profiles in one of the most easily accessible tissues, whole blood. We utilized an acute inflammation model to investigate the possibility of using a cDNA ... More
Methyl palmitate: inhibitor of phagocytosis in primary rat Kupffer cells.
AuthorsCai P, Kaphalia BS, Ansari GA,
JournalToxicology
PubMed ID15840433
Kupffer cells are involved in phagocytosis and known to release biologically active mediators during early events of liver injury. Such functional properties of Kupffer cells can be modulated by methyl palmitate (MP). Therefore, efficacy of MP to modulate Kupffer cell function was evaluated in cultured primary Kupffer cells from rat ... More
The genetic background of hypertensive, septic rats determines outcome improvement with antibiotic and G-CSF prophylaxis.
AuthorsBauhofer A, Tischer B, Middeke M, Plaul U, Lorenz W, Torossian A,
JournalShock
PubMed ID14501946
Hypertension is proposed as a risk factor among others (high age, diabetes mellitus, and pre- and intraoperative bleeding) for adverse outcomes, such as severe infections, leading to sepsis and to multiple organ failure as the most deleterious complication. Hypertension was modeled with spontaneous hypertensive rats (SHR) and Dahl salt-sensitive (DS) ... More
Metoclopramide: a novel adjunct for improving cardiac and hepatocellular functions after trauma-hemorrhage.
AuthorsJarrar D, Wang P, Song GY, Knöferl MW, Cioffi WG, Bland KI, Chaudry IH,
JournalAm J Physiol Endocrinol Metab
PubMed ID10644541
Although metoclopramide (MCP) administration after trauma-hemorrhage restores the depressed immune functions, it remains unknown whether this agent has any salutary effects on the depressed cardiovascular and hepatocellular functions under those conditions. Adult male Sprague-Dawley rats underwent a midline laparotomy (i.e., induction of soft-tissue trauma) and were then bled to and ... More
Prenatal exposure to maternal infection alters cytokine expression in the placenta, amniotic fluid, and fetal brain.
AuthorsUrakubo A, Jarskog LF, Lieberman JA, Gilmore JH,
JournalSchizophr Res
PubMed ID11163542
Prenatal exposure to infection appears to increase the risk of schizophrenia and other neurodevelopmental disorders. We have hypothesized that cytokines, generated in response to maternal infection, play a key mechanistic role in this association. E16 timed pregnancy rats were injected i.p. with Escherichia coli lipopolysaccharide (LPS) to model prenatal exposure ... More
Mechanism of the beneficial effects of pentoxifylline during sepsis: maintenance of adrenomedullin responsiveness and downregulation of proinflammatory cytokines.
AuthorsKoo DJ, Yoo P, Cioffi WG, Bland KI, Chaudry IH, Wang P,
JournalJ Surg Res
PubMed ID10816353
Although it is known that pentoxifylline (PTX) produces various beneficial effects during sepsis, it remains unknown whether this agent has any salutary effects on the depressed vascular responsiveness to adrenomedullin (ADM), a novel potent vasodilatory peptide, under such conditions. Adult male Sprague-Dawley rats were subjected to polymicrobial sepsis by cecal ... More
Pamapimod, a novel p38 mitogen-activated protein kinase inhibitor: preclinical analysis of efficacy and selectivity.
AuthorsHill RJ, Dabbagh K, Phippard D, Li C, Suttmann RT, Welch M, Papp E, Song KW, Chang KC, Leaffer D, Kim YN, Roberts RT, Zabka TS, Aud D, Dal Porto J, Manning AM, Peng SL, Goldstein DM, Wong BR,
JournalJ Pharmacol Exp Ther
PubMed ID18776065
P38alpha is a protein kinase that regulates the expression of inflammatory cytokines, suggesting a role in the pathogenesis of diseases such as rheumatoid arthritis (RA) or systemic lupus erythematosus. Here, we describe the preclinical pharmacology of pamapimod, a novel p38 mitogen-activated protein kinase inhibitor. Pamapimod inhibited p38alpha and p38beta enzymatic ... More
Impact of alcohol intoxication on hemodynamic, metabolic, and cytokine responses to hemorrhagic shock.
Alcohol intoxication is associated with a high incidence of traumatic injury, particularly in the young healthy population. The impact of alcohol intoxication on the immediate pathophysiologic response to injury has not been closely examined. We hypothesized that acute alcohol intoxication would aggravate the immediate outcome from hemorrhagic shock by impairing ... More
Microsomal cytochrome P450 levels and activities of isolated rat livers perfused with albumin.
AuthorsVuppugalla R, Shah RB, Chimalakonda AP, Fisher CW, Mehvar R,
JournalPharm Res
PubMed ID12608540
We recently showed that the perfusion of isolated rat livers with perfusates containing bovine serum albumin (BSA) would significantly stimulate the release of tumor necrosis factor (TNF)-alpha. Here, we hypothesize that BSA-induced increase in the release of TNF-alpha, and possibly other cytokines, would affect cytochrome P450 (CYP)-mediated drug metabolism. Rat ... More
Biomarkers in peripheral blood associated with vascular injury in Sprague-Dawley rats treated with the phosphodiesterase IV inhibitors SCH 351591 or SCH 534385.
AuthorsWeaver JL, Snyder R, Knapton A, Herman EH, Honchel R, Miller T, Espandiari P, Smith R, Gu YZ, Goodsaid FM, Rosenblum IY, Sistare FD, Zhang J, Hanig J,
JournalToxicol Pathol
PubMed ID18776166
Drug-associated vascular injury can be caused by phosphodiesterase (PDE) IV inhibitors and drugs from several other classes. The pathogenesis is poorly understood, but it appears to include vascular and innate immunological components. This research was undertaken to identify changes in peripheral blood associated with vascular injury caused by PDE IV ... More
Estrogen treatment following severe burn injury reduces brain inflammation and apoptotic signaling.
Patients with severe burn injury experience a rapid elevation in multiple circulating pro-inflammatory cytokines, with the levels correlating with both injury severity and outcome. Accumulations of these cytokines in animal models have been observed in remote organs, however data are lacking regarding early brain cytokine levels following burn injury, and ... More
Specific inhibition of mitochondrial oxidative stress suppresses inflammation and improves cardiac function in a rat pneumonia-related sepsis model.
AuthorsZang QS, Sadek H, Maass DL, Martinez B, Ma L, Kilgore JA, Williams NS, Frantz DE, Wigginton JG, Nwariaku FE, Wolf SE, Minei JP
JournalAm J Physiol Heart Circ Physiol
PubMed ID22408027
Using a mitochondria-targeted vitamin E (Mito-Vit-E) in a rat pneumonia-related sepsis model, we examined the role of mitochondrial reactive oxygen species in sepsis-mediated myocardial inflammation and subsequent cardiac contractile dysfunction. Sepsis was produced in adult male Sprague-Dawley rats via intratracheal injection of S. pneumonia (4 × 10(6) colony formation units ... More