SuperScript™ Plus Direct cDNA Labeling Module (no purification) with Alexa Fluor™-aha-dUTPs - FAQs

Can dU-containing DNA produced by amplification with dUTP be cut by restriction enzymes?

You will need to confirm with the manufacturer of the restriction enzyme you are using to find out whether or not it can recognize dUTP-containing DNA.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.