Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF.
AuthorsMacounová K, Cabrera CR, Yager P
JournalAnal Chem
PubMed ID11321320
'The use of microfluidic channels formed by two electrodes made of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentration and fractionation of proteins. The microchannels were 40 mm long with an electrode gap of 1.27 mm and a depth of 0.354 ... More
Dynamic microcompartmentation in synthetic cells.
AuthorsLong MS, Jones CD, Helfrich MR, Mangeney-Slavin LK, Keating CD
JournalProc Natl Acad Sci U S A
PubMed ID15788532
'An experimental model for cytoplasmic organization is presented. We demonstrate dynamic control over protein distribution within synthetic cells comprising a lipid bilayer membrane surrounding an aqueous polymer solution. This polymer solution generally exists as two immiscible aqueous phases. Protein partitioning between these phases leads to microcompartmentation, or heterogeneous protein distribution ... More
Flow cytometric analysis of the binding of eleven lectins to human T- and B-cells and to human T- and B-cell lines.
AuthorsMalin-Berdel J, Valet G, Thiel E, Forrester JA, Gürtler L
JournalCytometry
PubMed ID6609053
The relative surface binding of 11 lectins to human peripheral blood T- and B-lymphocytes, to Molt-4 and JM T-cell lines, and to 6410 and NC37 B-cell lines was determined by flow cytometry. The lectins from Lens culinaris (LCA), Ricinus communis (RCA), Arachis hypogaea (PNA), Abrus precatorius (APA), Ulex europaeus (UEA-F), ... More